AB61034

Anti-DKK1 antibody

Name: Anti-DKK1 antibody (ab61034) | Abcam
Brand: Abcam Limited
SKU: AB61034
Price: 545 USD
Availability: InStock
Rating: 5 (5 reviews)

(5 Reviews)

(26 Publications)

Rabbit Polyclonal DKK1 antibody. Suitable for IHC-P, IHC-Fr and reacts with Human, Mouse samples. Cited in 26 publications. Immunogen corresponding to Synthetic Peptide within Human DKK1 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

UNQ492/PRO1008, DKK1, Dickkopf-related protein 1, Dickkopf-1, Dkk-1, hDkk-1, SK

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody (AB61034)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody (AB61034)

ab61034 at 10μg/ml staining formalin fixed paraffin embedded Human Uterus (endometrium).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

IHC-Fr, IHC-P

applications

Immunogen

Synthetic Peptide within Human DKK1 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O94907

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": " Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C for 20 minutes. Remove from heat and let stand at room temperature in buffer for 20 minutes. Rinse in 1X TBS with Tween (TBST) for 1 minute at RT. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.7 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DKK1 protein also known as Dickkopf-1 is a secreted protein with a mass of approximately 28-30 kDa. It plays a mechanical role in inhibiting the Wnt signaling pathway by binding to LRP5/6 co-receptors preventing Wnt ligands from activating the pathway. DKK1 is expressed in various tissues including the bone brain and placenta reflecting its involvement in several biological processes. Its capacity to bind and neutralize the Wnt pathway components cements DKK1's role as a significant modulator of cellular functions.

Biological function summary

The inhibition of the Wnt signaling by DKK1 alters cell proliferation differentiation and migration. DKK1 does not function as part of a large multi-protein complex but interacts directly with specific co-receptors and modulates the activity of key signaling molecules. This regulatory mechanism controls processes like bone formation and neurogenesis indicating DKK1's broad impact in development and tissue homeostasis. Its expression pattern and biological role position it as an important player in maintaining proper cellular signaling environments.

Pathways

DKK1 is a critical player in the Wnt signaling and bone remodeling pathways. Through its interaction with Wnt pathway components like LRP5/6 DKK1 modulates these pathways affecting downstream proteins such as β-catenin and influencing gene transcription. In the bone remodeling context DKK1's ability to regulate osteoblast activity connects it to other related proteins like sclerostin contributing to bone density and health.

Elevated levels of DKK1 are observed in conditions like osteoporosis and cancer. In osteoporosis DKK1's inhibition of Wnt signaling leads to reduced bone formation implicating it alongside proteins like sclerostin in deteriorating bone health. In cancer DKK1's dysregulation can promote tumorigenesis by altering cell signaling and microenvironment interactions. These roles underline the potential of targeting DKK1 for therapeutic purposes in treating these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6 (PubMed : 22000856). DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (PubMed : 17143291). Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity (By similarity).

See full target information DKK1

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Publications (26)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 11:378 PubMed40804038

2025

AXL tyrosine kinase inhibitor TP-0903 induces ROS trigger neuroblastoma cell apoptosis via targeting the miR-335-3p/DKK1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Tsai-Yi Tseng,Shao-Hsuan Kao,Shun-Fa Yang,Yi-Chen Lin,Chu-Liang Lin,Juei-Liang Chen,Chien-Min Chen,Yi-Hsien Hsieh

Journal of periodontology 96:82-96 PubMed39012429

2024

Disparate effects of sclerostin deletion on alveolar bone and cellular cementum in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Aonjittra Phanrungsuwan,Michael B Chavez,Leena A Eltilib,Tamara N Kolli,Fatma F Mohamed,Michelle H Tan,Cristiane R Salmon,Francisco H Nociti,Brian L Foster

Aging and disease 14:919-936 PubMed37191410

2023

Osteoblastic Wls Ablation Protects Mice from Total Body Irradiation-Induced Impairments in Hematopoiesis and Bone Marrow Microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun-Jaung Sim,Han-Sol So,Sher Bahadur Poudel,Govinda Bhattarai,Eui-Sic Cho,Jeong-Chae Lee,Sung-Ho Kook

Biology 11: PubMed36552348

2022

Differential Expression of Dickkopf 1 and Periostin in Mouse Strains with High and Low Bone Mass.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Kerschan-Schindl,Victoria Schramek,Maria Butylina,Ursula Föger-Samwald,Peter Pietschmann

Tissue barriers 11:2039003 PubMed35262466

2022

GRP75 as a functional element of cholix transcytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Keyi Liu,Tom Hunter,Alistair Taverner,Kevin Yin,Julia MacKay,Kate Colebrook,Morgan Correia,Amandine Rapp,Randall J Mrsny

International journal of molecular sciences 23: PubMed35269944

2022

Dickkopf-1 Promotes Angiogenesis and is a Biomarker for Hepatic Stem Cell-like Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tsuyoshi Suda,Taro Yamashita,Hajime Sunagozaka,Hikari Okada,Kouki Nio,Yoshio Sakai,Tatsuya Yamashita,Eishiro Mizukoshi,Masao Honda,Shuichi Kaneko

The American journal of pathology 191:1020-1035 PubMed33705750

2021

The Canonical Wnt Signaling Pathway Inhibits the Glucocorticoid Receptor Signaling Pathway in the Trabecular Meshwork.

Applications

Unspecified application

Species

Unspecified reactive species

Chenna Kesavulu Sugali,Naga Pradeep Rayana,Jiannong Dai,Michael Peng,Sherri L Harris,Hannah C Webber,Shaohui Liu,Stephan G Dixon,Priyanka H Parekh,Elizabeth A Martin,Louis B Cantor,Ronald L Fellman,David G Godfrey,Michelle R Butler,Matthew E Emanuel,Davinder S Grover,Oluwatosin U Smith,Abbot F Clark,Vijay Krishna Raghunathan,Weiming Mao

eLife 9: PubMed33252038

2020

EHMT2 epigenetically suppresses Wnt signaling and is a potential target in embryonal rhabdomyosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ananya Pal,Jia Yu Leung,Gareth Chin Khye Ang,Vinay Kumar Rao,Luca Pignata,Huey Jin Lim,Maxime Hebrard,Kenneth Te Chang,Victor Km Lee,Ernesto Guccione,Reshma Taneja

Journal of neuroscience research 99:731-749 PubMed33197966

2020

Corneal nonmyelinating Schwann cells illuminated by single-cell transcriptomics and visualized by protein biomarkers.

Applications

Unspecified application

Species

Unspecified reactive species

Paola Bargagna-Mohan,Gwendolyn Schultz,Bruce Rheaume,Ephraim F Trakhtenberg,Paul Robson,Sonali Pal-Ghosh,Mary Ann Stepp,Katherine S Given,Wendy B Macklin,Royce Mohan

Arthritis & rheumatology (Hoboken, N.J.) 72:943-956 PubMed32362074

2020

Blockade of Discoidin Domain Receptor 2 as a Strategy for Reducing Inflammation and Joint Destruction in Rheumatoid Arthritis Via Altered Interleukin-15 and Dkk-1 Signaling in Fibroblast-Like Synoviocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Mu,Jin-Tao Gu,Tong-Lie Huang,Nan-Nan Liu,Hui Chen,Xin Bu,Zhao-Hui Zheng,Bo Jia,Jun Liu,Bao-Long Wang,Ying-Mei Wang,Zhen-Feng Zhu,Yong Zhang,Ying-Qi Zhang,Xiao-Chang Xue,Meng Li,Wei Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DKK1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody (AB61034)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (26)

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