Rabbit Polyclonal DKK1 antibody. Suitable for IHC-P, IHC-Fr and reacts with Human, Mouse samples. Cited in 22 publications. Immunogen corresponding to Synthetic Peptide within Human DKK1 conjugated to Keyhole Limpet Haemocyanin.
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- Biology 11:2022Differential Expression of Dickkopf 1 and Periostin in Mouse Strains with High and Low Bone Mass.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatharina Kerschan-Schindl,Victoria Schramek,Maria Butylina,Ursula Föger-Samwald,Peter PietschmannPubMed 36552348
- International journal of molecular sciences 23:2022Dickkopf-1 Promotes Angiogenesis and is a Biomarker for Hepatic Stem Cell-like Hepatocellular Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTsuyoshi Suda,Taro Yamashita,Hajime Sunagozaka,Hikari Okada,Kouki Nio,Yoshio Sakai,Tatsuya Yamashita,Eishiro Mizukoshi,Masao Honda,Shuichi KanekoPubMed 35269944
- The American journal of pathology 191:1020-10352021The Canonical Wnt Signaling Pathway Inhibits the Glucocorticoid Receptor Signaling Pathway in the Trabecular Meshwork.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenna Kesavulu Sugali,Naga Pradeep Rayana,Jiannong Dai,Michael Peng,Sherri L Harris,Hannah C Webber,Shaohui Liu,Stephan G Dixon,Priyanka H Parekh,Elizabeth A Martin,Louis B Cantor,Ronald L Fellman,David G Godfrey,Michelle R Butler,Matthew E Emanuel,Davinder S Grover,Oluwatosin U Smith,Abbot F Clark,Vijay Krishna Raghunathan,Weiming MaoPubMed 33705750
- eLife 9:2020EHMT2 epigenetically suppresses Wnt signaling and is a potential target in embryonal rhabdomyosarcoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnanya Pal et. al.PubMed 33252038
- Journal of neuroscience research 99:731-7492020Corneal nonmyelinating Schwann cells illuminated by single-cell transcriptomics and visualized by protein biomarkers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPaola Bargagna-Mohan,Gwendolyn Schultz,Bruce Rheaume,Ephraim F Trakhtenberg,Paul Robson,Sonali Pal-Ghosh,Mary Ann Stepp,Katherine S Given,Wendy B Macklin,Royce MohanPubMed 33197966
- Arthritis & rheumatology (Hoboken, N.J.) 72:943-9562020Blockade of Discoidin Domain Receptor 2 as a Strategy for Reducing Inflammation and Joint Destruction in Rheumatoid Arthritis Via Altered Interleukin-15 and Dkk-1 Signaling in Fibroblast-Like Synoviocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNan Mu,Jin-Tao Gu,Tong-Lie Huang,Nan-Nan Liu,Hui Chen,Xin Bu,Zhao-Hui Zheng,Bo Jia,Jun Liu,Bao-Long Wang,Ying-Mei Wang,Zhen-Feng Zhu,Yong Zhang,Ying-Qi Zhang,Xiao-Chang Xue,Meng Li,Wei ZhangPubMed 32362074
- Endocrinology 161:2020Inhibition of FGFR Signaling Partially Rescues Osteoarthritis in Mice Overexpressing High Molecular Weight FGF2 Isoforms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiping Xiao,Donyell Williams,Marja M HurleyPubMed 31901095
- International journal of molecular medicine 45:556-5682020Protective effects of human umbilical cord blood‑derived mesenchymal stem cells against dexamethasone‑induced apoptotic cell death in hair follicles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong Ho Bak,Esther Lee,Mi Ji Choi,Byung Chul Lee,Tae-Rin Kwon,Jong-Hwan Kim,Eun Su Jeon,Wonil Oh,Seog Kyun Mun,Byung Cheol Park,Jungtae Na,Beom Joon KimPubMed 31894311
- International journal of biological macromolecules 142:142-1512019Inhibition of protein phosphatase 2A attenuates titanium-particle induced suppression of bone formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiangliang Wang,Jiaxiang Bai,Qing Wang,Gaoran Ge,Jiayi Lin,Nanwei Xu,Chao Xu,Yaozeng Xu,Yuji Wang,Dechun GengPubMed 31521663
- Scientific reports 8:179752018Human ex vivo 3D bone model recapitulates osteocyte response to metastatic prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSaba Choudhary et. al.PubMed 30568232
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.7
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human DKK1 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link O94907
Reactivity data
Select an application| IHC-P | IHC-Fr | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Predicted | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes
Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6 (PubMed:22000856). DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (PubMed:17143291). Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity (By similarity).
Alternative names
UNQ492/PRO1008, DKK1, Dickkopf-related protein 1, Dickkopf-1, Dkk-1, hDkk-1, SK
Recommended products
Rabbit Polyclonal DKK1 antibody. Suitable for IHC-P, IHC-Fr and reacts with Human, Mouse samples. Cited in 22 publications. Immunogen corresponding to Synthetic Peptide within Human DKK1 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.7
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human DKK1 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link O94907
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The DKK1 protein also known as Dickkopf-1 is a secreted protein with a mass of approximately 28-30 kDa. It plays a mechanical role in inhibiting the Wnt signaling pathway by binding to LRP5/6 co-receptors preventing Wnt ligands from activating the pathway. DKK1 is expressed in various tissues including the bone brain and placenta reflecting its involvement in several biological processes. Its capacity to bind and neutralize the Wnt pathway components cements DKK1's role as a significant modulator of cellular functions.
- Biological function summary
The inhibition of the Wnt signaling by DKK1 alters cell proliferation differentiation and migration. DKK1 does not function as part of a large multi-protein complex but interacts directly with specific co-receptors and modulates the activity of key signaling molecules. This regulatory mechanism controls processes like bone formation and neurogenesis indicating DKK1's broad impact in development and tissue homeostasis. Its expression pattern and biological role position it as an important player in maintaining proper cellular signaling environments.
- Pathways
DKK1 is a critical player in the Wnt signaling and bone remodeling pathways. Through its interaction with Wnt pathway components like LRP5/6 DKK1 modulates these pathways affecting downstream proteins such as β-catenin and influencing gene transcription. In the bone remodeling context DKK1's ability to regulate osteoblast activity connects it to other related proteins like sclerostin contributing to bone density and health.
- Associated diseases and disorders
Elevated levels of DKK1 are observed in conditions like osteoporosis and cancer. In osteoporosis DKK1's inhibition of Wnt signaling leads to reduced bone formation implicating it alongside proteins like sclerostin in deteriorating bone health. In cancer DKK1's dysregulation can promote tumorigenesis by altering cell signaling and microenvironment interactions. These roles underline the potential of targeting DKK1 for therapeutic purposes in treating these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody (ab61034)
ab61034 at 10μg/ml staining formalin fixed paraffin embedded Human Uterus (endometrium).
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