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Rabbit Recombinant Monoclonal DKK1 antibody. Suitable for IP, ICC/IF, WB and reacts with Mouse, Human samples. Cited in 1 publication.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow Cyt (Intra)ICC/IFIHC-PWB
Human
Expected
Not recommended
Expected
Not recommended
Tested
Mouse
Tested
Not recommended
Tested
Not recommended
Tested
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Mouse

Dilution info

1/30

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/50 - 1/1000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

-

Target data

Function

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6 (PubMed:22000856). DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (PubMed:17143291). Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity (By similarity).

Alternative names

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Rabbit Recombinant Monoclonal DKK1 antibody. Suitable for IP, ICC/IF, WB and reacts with Mouse, Human samples. Cited in 1 publication.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR26160-102

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

The inhibition of the Wnt signaling by DKK1 alters cell proliferation differentiation and migration. DKK1 does not function as part of a large multi-protein complex but interacts directly with specific co-receptors and modulates the activity of key signaling molecules. This regulatory mechanism controls processes like bone formation and neurogenesis indicating DKK1's broad impact in development and tissue homeostasis. Its expression pattern and biological role position it as an important player in maintaining proper cellular signaling environments.

Activity summary

The DKK1 protein also known as Dickkopf-1 is a secreted protein with a mass of approximately 28-30 kDa. It plays a mechanical role in inhibiting the Wnt signaling pathway by binding to LRP5/6 co-receptors preventing Wnt ligands from activating the pathway. DKK1 is expressed in various tissues including the bone brain and placenta reflecting its involvement in several biological processes. Its capacity to bind and neutralize the Wnt pathway components cements DKK1's role as a significant modulator of cellular functions.

Pathways

DKK1 is a critical player in the Wnt signaling and bone remodeling pathways. Through its interaction with Wnt pathway components like LRP5/6 DKK1 modulates these pathways affecting downstream proteins such as β-catenin and influencing gene transcription. In the bone remodeling context DKK1's ability to regulate osteoblast activity connects it to other related proteins like sclerostin contributing to bone density and health.

Associated diseases and disorders

Elevated levels of DKK1 are observed in conditions like osteoporosis and cancer. In osteoporosis DKK1's inhibition of Wnt signaling leads to reduced bone formation implicating it alongside proteins like sclerostin in deteriorating bone health. In cancer DKK1's dysregulation can promote tumorigenesis by altering cell signaling and microenvironment interactions. These roles underline the potential of targeting DKK1 for therapeutic purposes in treating these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunoprecipitation - Anti-DKK1 antibody [EPR26160-102] (ab307367), expandable thumbnail

    Immunoprecipitation - Anti-DKK1 antibody [EPR26160-102] (ab307367)

    DKK1 was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate 10 ug with AB307367 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using AB307367 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(AB131366) was used at 1/5000 dilution. Lane 1: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate 10 ug
    Lane 2: ABAB307367 IP in Neuro-2a whole cell lysate
    Lane 3:RABbit monoclonal IgG (AB172730) instead of AB307367 in Neuro-2a whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 seconds
    The band beneath the target band is likely to be degraded target fragments.

    All lanes: Immunoprecipitation - Anti-DKK1 antibody [EPR26160-102] (AB307367) at 1/1000 dilution

    Lane 1: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate 10 μg

    Lane 2: Neuro-2a whole cell lysate

    Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366) at 1/5000 dilution

    Observed band size: 35 kDa

    Exposure time: 3s

  • Western blot - Anti-DKK1 antibody [EPR26160-102] (ab307367), expandable thumbnail

    Western blot - Anti-DKK1 antibody [EPR26160-102] (ab307367)

    Blocking and diluting buffer and concentration: 5% NFDM/TBSTLysates were freshly made and used for Western blotting immediately to minimize protein degradation.

    The identity of the higher MW band at approximately 75 kDa is unknown.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 14695885).
    Exposure time: Lane 1-3: 37 seconds, Lane 4-6: 81 seconds.

    All lanes: Western blot - Anti-DKK1 antibody [EPR26160-102] (AB307367) at 1/1000 dilution

    All lanes: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate 20 μg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution

    Observed band size: 35 kDa

    Exposure time: 158s

  • Western blot - Anti-DKK1 antibody [EPR26160-102] (ab307367), expandable thumbnail

    Western blot - Anti-DKK1 antibody [EPR26160-102] (ab307367)

    Blocking and diluting buffer and concentration: 5% NFDM/TBSTLow expression: C2C12 (PMID: 18305214), 3T3-L1 (PMID: 19943155), HEK-293 (PMID: 18502762), Jurkat (PMID: 20653953)

    This blot was developed using a high sensitivity ECL substrate.

    The identity of the higher MW band at approximately 75 kDa is unknown.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 14695885).
    Exposure time:

    All lanes: Western blot - Anti-DKK1 antibody [EPR26160-102] (AB307367) at 1/1000 dilution

    Lane 1: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 20 μg

    Lane 2: C2C12 (mouse myoblasts myoblast) whole cell lysate 20 μg

    Lane 3: 3T3-L1 (mouse embryonic fibroblast) differentiated into adipocyte-like cells whole cell lysate 20 μg

    Lane 4: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate 20 μg

    Lane 5: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 20 μg

    Lane 6: Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate 20 μg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 35 kDa

    Exposure time: 37s

  • Immunocytochemistry/ Immunofluorescence - Anti-DKK1 antibody [EPR26160-102] (ab307367), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-DKK1 antibody [EPR26160-102] (ab307367)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeABilized Neuro-2a (mouse neuroblastoma neuroblast) cells lABelling DKK1 with AB307367 at 1/50 (10.78 ug/ml) dilution, followed by AB150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing cytoplasmic staining in Neuro-2a cell line.Low expression: C2C12 (PMID:?18305214).Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. AB195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor? 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is AB150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/mL dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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