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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Recombinant Monoclonal DKK1 antibody. Suitable for IP, ICC/IF, WB and reacts with Mouse, Human samples. Cited in 1 publication.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IP | Flow Cyt (Intra) | ICC/IF | IHC-P | WB | |
---|---|---|---|---|---|
Human | Expected | Not recommended | Expected | Not recommended | Tested |
Mouse | Tested | Not recommended | Tested | Not recommended | Tested |
Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/30 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/50 - 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6 (PubMed:22000856). DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (PubMed:17143291). Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity (By similarity).
Dickkopf-related protein 1, Dickkopf-1, Dkk-1, hDkk-1, SK, DKK1, UNQ492/PRO1008
Rabbit Recombinant Monoclonal DKK1 antibody. Suitable for IP, ICC/IF, WB and reacts with Mouse, Human samples. Cited in 1 publication.
Dickkopf-related protein 1, Dickkopf-1, Dkk-1, hDkk-1, SK, DKK1, UNQ492/PRO1008
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR26160-102
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
The inhibition of the Wnt signaling by DKK1 alters cell proliferation differentiation and migration. DKK1 does not function as part of a large multi-protein complex but interacts directly with specific co-receptors and modulates the activity of key signaling molecules. This regulatory mechanism controls processes like bone formation and neurogenesis indicating DKK1's broad impact in development and tissue homeostasis. Its expression pattern and biological role position it as an important player in maintaining proper cellular signaling environments.
The DKK1 protein also known as Dickkopf-1 is a secreted protein with a mass of approximately 28-30 kDa. It plays a mechanical role in inhibiting the Wnt signaling pathway by binding to LRP5/6 co-receptors preventing Wnt ligands from activating the pathway. DKK1 is expressed in various tissues including the bone brain and placenta reflecting its involvement in several biological processes. Its capacity to bind and neutralize the Wnt pathway components cements DKK1's role as a significant modulator of cellular functions.
DKK1 is a critical player in the Wnt signaling and bone remodeling pathways. Through its interaction with Wnt pathway components like LRP5/6 DKK1 modulates these pathways affecting downstream proteins such as β-catenin and influencing gene transcription. In the bone remodeling context DKK1's ability to regulate osteoblast activity connects it to other related proteins like sclerostin contributing to bone density and health.
Elevated levels of DKK1 are observed in conditions like osteoporosis and cancer. In osteoporosis DKK1's inhibition of Wnt signaling leads to reduced bone formation implicating it alongside proteins like sclerostin in deteriorating bone health. In cancer DKK1's dysregulation can promote tumorigenesis by altering cell signaling and microenvironment interactions. These roles underline the potential of targeting DKK1 for therapeutic purposes in treating these diseases.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
DKK1 was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate 10 ug with AB307367 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using AB307367 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(AB131366) was used at 1/5000 dilution. Lane 1: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate 10 ug
Lane 2: ABAB307367 IP in Neuro-2a whole cell lysate
Lane 3:RABbit monoclonal IgG (AB172730) instead of AB307367 in Neuro-2a whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds
The band beneath the target band is likely to be degraded target fragments.
All lanes: Immunoprecipitation - Anti-DKK1 antibody [EPR26160-102] (AB307367) at 1/1000 dilution
Lane 1: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate 10 μg
Lane 2: Neuro-2a whole cell lysate
Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366) at 1/5000 dilution
Observed band size: 35 kDa
Exposure time: 3s
Blocking and diluting buffer and concentration: 5% NFDM/TBSTLysates were freshly made and used for Western blotting immediately to minimize protein degradation.
The identity of the higher MW band at approximately 75 kDa is unknown.
The molecular weight observed is consistent with what has been described in the literature (PMID: 14695885).
Exposure time: Lane 1-3: 37 seconds, Lane 4-6: 81 seconds.
All lanes: Western blot - Anti-DKK1 antibody [EPR26160-102] (AB307367) at 1/1000 dilution
All lanes: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate 20 μg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution
Observed band size: 35 kDa
Exposure time: 158s
Blocking and diluting buffer and concentration: 5% NFDM/TBSTLow expression: C2C12 (PMID: 18305214), 3T3-L1 (PMID: 19943155), HEK-293 (PMID: 18502762), Jurkat (PMID: 20653953)
This blot was developed using a high sensitivity ECL substrate.
The identity of the higher MW band at approximately 75 kDa is unknown.
The molecular weight observed is consistent with what has been described in the literature (PMID: 14695885).
Exposure time:
All lanes: Western blot - Anti-DKK1 antibody [EPR26160-102] (AB307367) at 1/1000 dilution
Lane 1: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 20 μg
Lane 2: C2C12 (mouse myoblasts myoblast) whole cell lysate 20 μg
Lane 3: 3T3-L1 (mouse embryonic fibroblast) differentiated into adipocyte-like cells whole cell lysate 20 μg
Lane 4: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate 20 μg
Lane 5: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 20 μg
Lane 6: Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate 20 μg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 35 kDa
Exposure time: 37s
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeABilized Neuro-2a (mouse neuroblastoma neuroblast) cells lABelling DKK1 with AB307367 at 1/50 (10.78 ug/ml) dilution, followed by AB150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing cytoplasmic staining in Neuro-2a cell line.Low expression: C2C12 (PMID:?18305214).Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. AB195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor? 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is AB150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/mL dilution.
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