Rabbit Recombinant Monoclonal DKK1 antibody. Carrier free. Suitable for IP, ICC/IF, WB and reacts with Mouse, Human samples.
Images
![Western blot - Anti-DKK1 antibody [EPR26160-102] - BSA and Azide free (AB307368), expandable thumbnail](https://content.abcam.com/products/images/dkk1-antibody-epr26160-102-bsa-and-azide-free-ab307368--western-blot-img405490.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DKK1 antibody [EPR26160-102] - BSA and Azide free (AB307368)")
![Immunoprecipitation - Anti-DKK1 antibody [EPR26160-102] - BSA and Azide free (AB307368), expandable thumbnail](https://content.abcam.com/products/images/dkk1-antibody-epr26160-102-bsa-and-azide-free-ab307368--immunoprecipitation-img406396.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-DKK1 antibody [EPR26160-102] - BSA and Azide free (AB307368)")
![Western blot - Anti-DKK1 antibody [EPR26160-102] - BSA and Azide free (AB307368), expandable thumbnail](https://content.abcam.com/products/images/dkk1-antibody-epr26160-102-bsa-and-azide-free-ab307368--western-blot-img405491.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DKK1 antibody [EPR26160-102] - BSA and Azide free (AB307368)")
![Immunocytochemistry/ Immunofluorescence - Anti-DKK1 antibody [EPR26160-102] - BSA and Azide free (AB307368), expandable thumbnail](https://content.abcam.com/products/images/dkk1-antibody-epr26160-102-bsa-and-azide-free-ab307368--immunocytochemistry-immunofluorescence-img405488.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-DKK1 antibody [EPR26160-102] - BSA and Azide free (AB307368)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt (Intra) | ICC/IF | IHC-P | WB | |
|---|---|---|---|---|---|
| Human | Expected | Not recommended | Expected | Not recommended | Tested |
| Mouse | Tested | Not recommended | Tested | Not recommended | Tested |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Target data
Function
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6 (PubMed:22000856). DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (PubMed:17143291). Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity (By similarity).
Alternative names
UNQ492/PRO1008, DKK1, Dickkopf-related protein 1, Dickkopf-1, Dkk-1, hDkk-1, SK
Recommended products
Rabbit Recombinant Monoclonal DKK1 antibody. Carrier free. Suitable for IP, ICC/IF, WB and reacts with Mouse, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26160-102
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The DKK1 protein also known as Dickkopf-1 is a secreted protein with a mass of approximately 28-30 kDa. It plays a mechanical role in inhibiting the Wnt signaling pathway by binding to LRP5/6 co-receptors preventing Wnt ligands from activating the pathway. DKK1 is expressed in various tissues including the bone brain and placenta reflecting its involvement in several biological processes. Its capacity to bind and neutralize the Wnt pathway components cements DKK1's role as a significant modulator of cellular functions.
- Biological function summary
The inhibition of the Wnt signaling by DKK1 alters cell proliferation differentiation and migration. DKK1 does not function as part of a large multi-protein complex but interacts directly with specific co-receptors and modulates the activity of key signaling molecules. This regulatory mechanism controls processes like bone formation and neurogenesis indicating DKK1's broad impact in development and tissue homeostasis. Its expression pattern and biological role position it as an important player in maintaining proper cellular signaling environments.
- Pathways
DKK1 is a critical player in the Wnt signaling and bone remodeling pathways. Through its interaction with Wnt pathway components like LRP5/6 DKK1 modulates these pathways affecting downstream proteins such as β-catenin and influencing gene transcription. In the bone remodeling context DKK1's ability to regulate osteoblast activity connects it to other related proteins like sclerostin contributing to bone density and health.
- Associated diseases and disorders
Elevated levels of DKK1 are observed in conditions like osteoporosis and cancer. In osteoporosis DKK1's inhibition of Wnt signaling leads to reduced bone formation implicating it alongside proteins like sclerostin in deteriorating bone health. In cancer DKK1's dysregulation can promote tumorigenesis by altering cell signaling and microenvironment interactions. These roles underline the potential of targeting DKK1 for therapeutic purposes in treating these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-DKK1 antibody [EPR26160-102] - BSA and Azide free (ab307368), expandable thumbnail](https://content.abcam.com/products/images/dkk1-antibody-epr26160-102-bsa-and-azide-free-ab307368--western-blot-img405490.jpg "Western blot - Anti-DKK1 antibody [EPR26160-102] - BSA and Azide free (ab307368)")
Western blot - Anti-DKK1 antibody [EPR26160-102] - BSA and Azide free (ab307368)
This data was developed using 307367, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: C2C12 (PMID: 18305214), 3T3-L1 (PMID: 19943155), HEK-293 (PMID: 18502762), Jurkat (PMID: 20653953)
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
The identity of the higher MW band at approximately 75 kDa is unknown.
The molecular weight observed is consistent with what has been described in the literature (PMID: 14695885).
Exposure time:
Lane 1-3: 37 seconds
Lane 4-6: 81 seconds
All lanes: Western blot - Anti-DKK1 antibody [EPR26160-102] (Anti-DKK1 antibody [EPR26160-102] ab307367) at 1/1000 dilution
Lane 1: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg
Lane 2: C2C12 (mouse myoblasts myoblast) whole cell lysate at 20 µg
Lane 3: 3T3-L1 (mouse embryonic fibroblast) differentiated into adipocyte-like cells whole cell lysate at 20 µg
Lane 4: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysat at 20 µg
Lane 5: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 20 μg at 20 µg
Lane 6: Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 35 kDa
![Immunoprecipitation - Anti-DKK1 antibody [EPR26160-102] - BSA and Azide free (ab307368), expandable thumbnail](https://content.abcam.com/products/images/dkk1-antibody-epr26160-102-bsa-and-azide-free-ab307368--immunoprecipitation-img406396.jpg "Immunoprecipitation - Anti-DKK1 antibody [EPR26160-102] - BSA and Azide free (ab307368)")
Immunoprecipitation - Anti-DKK1 antibody [EPR26160-102] - BSA and Azide free (ab307368)
This data was developed using Anti-DKK1 antibody [EPR26160-102] ab307367, the same antibody clone in a different buffer formulation.
DKK1 was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate 10 ug with Anti-DKK1 antibody [EPR26160-102] ab307367 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-DKK1 antibody [EPR26160-102] ab307367 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate 10 ug
Lane 2: ABAB307367 IP in Neuro-2a whole cell lysate
Lane 3:RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-DKK1 antibody [EPR26160-102] ab307367 in Neuro-2a whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds
The band beneath the target band is likely to be degraded target fragments.All lanes: Immunoprecipitation - Anti-DKK1 antibody [EPR26160-102] (Anti-DKK1 antibody [EPR26160-102] ab307367) at 1/1000 dilution
Lane 1: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate 10 μg
Lane 2: Neuro-2a whole cell lysate
Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 35 kDa
Exposure time: 3s
![Western blot - Anti-DKK1 antibody [EPR26160-102] - BSA and Azide free (ab307368), expandable thumbnail](https://content.abcam.com/products/images/dkk1-antibody-epr26160-102-bsa-and-azide-free-ab307368--western-blot-img405491.jpg "Western blot - Anti-DKK1 antibody [EPR26160-102] - BSA and Azide free (ab307368)")
Western blot - Anti-DKK1 antibody [EPR26160-102] - BSA and Azide free (ab307368)
This data was developed using 307367, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
The identity of the higher MW band at approximately 75 kDa is unknown.
The molecular weight observed is consistent with what has been described in the literature (PMID: 14695885).
Lane 1-3: 37 seconds, Lane 4-6: 81 seconds.
Exposure time:All lanes: Western blot - Anti-DKK1 antibody [EPR26160-102] (Anti-DKK1 antibody [EPR26160-102] ab307367) at 1/1000 dilution
All lanes: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate 20 μg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 35 kDa
Exposure time: 158s
![Immunocytochemistry/ Immunofluorescence - Anti-DKK1 antibody [EPR26160-102] - BSA and Azide free (ab307368), expandable thumbnail](https://content.abcam.com/products/images/dkk1-antibody-epr26160-102-bsa-and-azide-free-ab307368--immunocytochemistry-immunofluorescence-img405488.jpg "Immunocytochemistry/ Immunofluorescence - Anti-DKK1 antibody [EPR26160-102] - BSA and Azide free (ab307368)")
Immunocytochemistry/ Immunofluorescence - Anti-DKK1 antibody [EPR26160-102] - BSA and Azide free (ab307368)
This data was developed using Anti-DKK1 antibody [EPR26160-102] ab307367, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeABilized Neuro-2a (mouse neuroblastoma neuroblast) cells lABelling DKK1 with Anti-DKK1 antibody [EPR26160-102] ab307367 at 1/50 (10.78 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing cytoplasmic staining in Neuro-2a cell line.Low expression: C2C12 (PMID:?18305214).Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor? 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/mL dilution.
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