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Rabbit Recombinant Monoclonal DKK1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 48 publications.


Images

Western blot - Anti-DKK1 antibody [EPR4759] (AB109416), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody [EPR4759] (AB109416), expandable thumbnail
  • Western blot - Anti-DKK1 antibody [EPR4759] (AB109416), expandable thumbnail
  • Western blot - Anti-DKK1 antibody [EPR4759] (AB109416), expandable thumbnail
  • Western blot - Anti-DKK1 antibody [EPR4759] (AB109416), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytICC/IFWBIHC-P
Human
Not recommended
Not recommended
Tested
Tested

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/800
Notes

1/250.00000 - 1/500.00000

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

3 products for Alternative Product

1 product for Alternative Version

Target data

Function

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6 (PubMed:22000856). DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (PubMed:17143291). Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity (By similarity).

Alternative names

UNQ492/PRO1008, DKK1, Dickkopf-related protein 1, Dickkopf-1, Dkk-1, hDkk-1, SK

Recommended products

Rabbit Recombinant Monoclonal DKK1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 48 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR4759
Purification technique
Affinity purification Protein A
Specificity

Although our internal data shows that this antibody is not suitable to detect DKK1 in rats, this antibody has been referenced in PMID 29299122 and 25469240 to work with rat derived cells. Please contact technical@abcam.com for further information.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The DKK1 protein also known as Dickkopf-1 is a secreted protein with a mass of approximately 28-30 kDa. It plays a mechanical role in inhibiting the Wnt signaling pathway by binding to LRP5/6 co-receptors preventing Wnt ligands from activating the pathway. DKK1 is expressed in various tissues including the bone brain and placenta reflecting its involvement in several biological processes. Its capacity to bind and neutralize the Wnt pathway components cements DKK1's role as a significant modulator of cellular functions.

Biological function summary

The inhibition of the Wnt signaling by DKK1 alters cell proliferation differentiation and migration. DKK1 does not function as part of a large multi-protein complex but interacts directly with specific co-receptors and modulates the activity of key signaling molecules. This regulatory mechanism controls processes like bone formation and neurogenesis indicating DKK1's broad impact in development and tissue homeostasis. Its expression pattern and biological role position it as an important player in maintaining proper cellular signaling environments.

Pathways

DKK1 is a critical player in the Wnt signaling and bone remodeling pathways. Through its interaction with Wnt pathway components like LRP5/6 DKK1 modulates these pathways affecting downstream proteins such as β-catenin and influencing gene transcription. In the bone remodeling context DKK1's ability to regulate osteoblast activity connects it to other related proteins like sclerostin contributing to bone density and health.

Associated diseases and disorders

Elevated levels of DKK1 are observed in conditions like osteoporosis and cancer. In osteoporosis DKK1's inhibition of Wnt signaling leads to reduced bone formation implicating it alongside proteins like sclerostin in deteriorating bone health. In cancer DKK1's dysregulation can promote tumorigenesis by altering cell signaling and microenvironment interactions. These roles underline the potential of targeting DKK1 for therapeutic purposes in treating these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-DKK1 antibody [EPR4759] (ab109416), expandable thumbnail

    Western blot - Anti-DKK1 antibody [EPR4759] (ab109416)

    False colour image of Western blot: Anti-DKK1 antibody [EPR4759] staining at 1/2000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-DKK1 antibody [EPR4759] (ab109416) at 1/2000 dilution

    Lane 1: Caco-2 cell lysate

    Lane 2: PC-3 cell lysate

    Lane 3: U-2 OS cell lysate

    Lane 4: MOLT4 cell lysate

    Lane 5: HEK-293 cell lysate

    Performed under reducing conditions.

    Predicted band size: 29 kDa

    Observed band size: 30-45 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody [EPR4759] (ab109416), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody [EPR4759] (ab109416)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling DKK1 with Purified ab109416 at 1:800 dilution (1.04 µg/ml). Heat mediated antigen retrieval was performed using using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain

  • Western blot - Anti-DKK1 antibody [EPR4759] (ab109416), expandable thumbnail

    Western blot - Anti-DKK1 antibody [EPR4759] (ab109416)

    Blocking/Diluting buffer and concentration 5% NFDM/TBST.

    HEK-293 and 293T cell are weak expressed cell lines for DKK1 protein (PMID: 18502762, PMID: 15378020).

    All lanes: Western blot - Anti-DKK1 antibody [EPR4759] (ab109416) at 1/10000 dilution

    Lane 1: Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 29 kDa

    Observed band size: 29-40 kDa

    Exposure time: 180s

  • Western blot - Anti-DKK1 antibody [EPR4759] (ab109416), expandable thumbnail

    Western blot - Anti-DKK1 antibody [EPR4759] (ab109416)

    Blocking/Diluting buffer and concentration 5% NFDM /TBST

    Exposure time: 5.5 seconds

    All lanes: Western blot - Anti-DKK1 antibody [EPR4759] (ab109416) at 1/1000 dilution

    All lanes: His-Tagged human DKK1 (aa 32 to 266) recombinant protein, 100ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 29 kDa

  • Western blot - Anti-DKK1 antibody [EPR4759] (ab109416), expandable thumbnail

    Western blot - Anti-DKK1 antibody [EPR4759] (ab109416)

    Blocking and diluting buffer: 5% NFDM/TBST.

    The smear bands detected by ab109416 are similar to those reported by the papers (PMID: 18546262, 28090290, 24008318), which should be due to the glycosylation of DKK1.

    All lanes: Western blot - Anti-DKK1 antibody [EPR4759] (ab109416) at 1/2000 dilution

    Lane 1: A549 (Human lung carcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 2: PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 3: NCI-H460 (Human Large Cell Lung Cancer epithelial cell) whole cell lysates at 20 µg

    Lane 4: DU145 (Human prostate carcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 5: SK-OV-3 (Human ovarian cancer epithelial cell) whole cell lysates at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 29 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody [EPR4759] (ab109416), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody [EPR4759] (ab109416)

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue using 1/100 unpurified ab109416.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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