AB2459

Anti-Dkk3 antibody

5

(3 Reviews)

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(7 Publications)

Goat Polyclonal Dkk3 antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human DKK3 aa 300 to C-terminus.

View Alternative Names

REIC, UNQ258/PRO295, DKK3, Dickkopf-related protein 3, Dickkopf-3, Dkk-3, hDkk-3

1 Images

Western blot - Anti-Dkk3 antibody (AB2459)

WB

Supplier Data

Western blot - Anti-Dkk3 antibody (AB2459)

All lanes:

Western blot - Anti-Dkk3 antibody (ab2459) at 2 µg/mL

All lanes:

Human Cerebellum lysate at 35 µg

Predicted band size: 38 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

Immunogen

Synthetic Peptide within Human DKK3 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is expected to recognise the 350 aa isoform only.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.03-0.1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity).

See full target information DKK3

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 10:2553-2570 PubMed32194819

2020

HIF-1ɑ-regulated miR-1275 maintains stem cell-like phenotypes and promotes the progression of LUAD by simultaneously activating Wnt/β-catenin and Notch signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Neng Jiang,Chang Zou,Ying Zhu,Yifeng Luo,Lili Chen,Yiyan Lei,Kejing Tang,Yu Sun,Wenhui Zhang,Shuhua Li,Qiong He,Jianwen Zhou,Yangshan Chen,Jiping Luo,Wenting Jiang,Zunfu Ke

Scientific reports 9:19139 PubMed31844121

2019

LINC00459 sponging miR-218 to elevate DKK3 inhibits proliferation and invasion in melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhua Yang,Wenxian Xu,Zhuojun Zheng,Zhihai Cao

Nature communications 10:134 PubMed30635555

2019

Single-cell transcriptomic analysis of mouse neocortical development.

Applications

Unspecified application

Species

Unspecified reactive species

Lipin Loo,Jeremy M Simon,Lei Xing,Eric S McCoy,Jesse K Niehaus,Jiami Guo,E S Anton,Mark J Zylka

PloS one 11:e0154531 PubMed27140416

2016

Conserved Senescence Associated Genes and Pathways in Primary Human Fibroblasts Detected by RNA-Seq.

Applications

WB

Species

Unspecified reactive species

S Marthandan,M Baumgart,S Priebe,M Groth,J Schaer,C Kaether,R Guthke,A Cellerino,M Platzer,S Diekmann,P Hemmerich

BioMed research international 2015:731938 PubMed26339636

2015

Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts.

Applications

WB

Species

Unspecified reactive species

Shiva Marthandan,Steffen Priebe,Mario Baumgart,Marco Groth,Alessandro Cellerino,Reinhard Guthke,Peter Hemmerich,Stephan Diekmann

PloS one 9:e102559 PubMed25029272

2014

Pax6 regulates the expression of Dkk3 in murine and human cell lines, and altered responses to Wnt signaling are shown in FlpIn-3T3 cells stably expressing either the Pax6 or the Pax6(5a) isoform.

Applications

WB, WB, ICC/IF, ICC/IF

Species

Human, Mouse, Human, Mouse

Siri Forsdahl,Yury Kiselev,Rune Hogseth,Janne E Mjelle,Ingvild Mikkola

British journal of cancer 99:663-9 PubMed18665176

2008

MT1-MMP regulates urothelial cell invasion via transcriptional regulation of Dickkopf-3.

Applications

WB

Species

Human

K Saeb-Parsy,A Veerakumarasivam,M J Wallard,N Thorne,Y Kawano,G Murphy,D E Neal,I G Mills,J D Kelly

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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