AB186409

Anti-Dkk3 antibody [EPR15611]

RabMAb
Recombinant
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(18 Publications)

Rabbit Recombinant Monoclonal Dkk3 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 18 publications.

View Alternative Names

REIC, UNQ258/PRO295, DKK3, Dickkopf-related protein 3, Dickkopf-3, Dkk-3, hDkk-3

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-Dkk3 antibody [EPR15611] (AB186409)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Dkk3 antibody [EPR15611] (AB186409)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Dkk3 with ab186409 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

Supplier Data

Immunoprecipitation - Anti-Dkk3 antibody [EPR15611] (AB186409)

Western blot analysis of Dkk3 in Human fetal brain lysate immunoprecipitated with ab186409 at 1/50 dilution (Lane 1). Lane 2 : PBS instead of Human fetal brain lysate.

Secondary antibody : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-Dkk3 antibody [EPR15611] (ab186409)

Predicted band size: 38 kDa,56 kDa

Observed band size: 60 kDa

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Lab

Western blot - Anti-Dkk3 antibody [EPR15611] (AB186409)

Blocking buffer and concentration : 5% NFDM/TBST Diluting buffer and concentration : 5% NFDM/TBST DKK3 shows 50-70 kDa bands, which is due to glycosylation (PMID : 28674110. PMID : 28738084). DKK3 is not expressed in PC-3 and BxPC-3. Negative control : PC-3 (PMID : 22941469), BxPC-3 (PMID : 28470144, PMID : 26395974, PMID : 34391478).

All lanes:

Western blot - Anti-Dkk3 antibody [EPR15611] (ab186409) at 1/1000 dilution

Lane 1:

A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

BxPC-3 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

Human heart tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 38 kDa

false

Exposure time: 10s

Supplier Data

Western blot - Anti-Dkk3 antibody [EPR15611] (AB186409)

All lanes:

Western blot - Anti-Dkk3 antibody [EPR15611] (ab186409) at 1/10000 dilution

All lanes:

Human fetal brain lysate at 20 µg

Secondary

All lanes:

Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

Predicted band size: 38 kDa

Observed band size: 60 kDa

false

Supplier Data

Western blot - Anti-Dkk3 antibody [EPR15611] (AB186409)

All lanes:

Western blot - Anti-Dkk3 antibody [EPR15611] (ab186409) at 1/5000 dilution

Lane 1:

Human fetal kidney lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 38 kDa

Observed band size: 60 kDa

false

Carrier free

Anti-Dkk3 antibody [EPR15611] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15611

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB, ICC/IF

applications

Complementary Products

Assay Kits

AB236710

Human DKK3 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human DKK3 ELISA Kit (AB236710)

Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Primary Antibodies

AB32572

Anti-beta Catenin antibody [E247] - ChIP Grade

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [E247] - ChIP Grade (AB32572)

Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50 - 1/70", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity).

See full target information DKK3

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

STAR protocols 6:103760 PubMed40238632

2025

Protocol for characterizing craniopharyngioma subtypes and their microenvironments using single-cell RNA sequencing and immunohistochemistry.

Applications

Unspecified application

Species

Unspecified reactive species

Takashi Kono,Tatsuma Matsuda,Masanori Fujimoto,Yuki Taki,Ikki Sakuma,Naoko Hashimoto,Yasuhiro Nakamura,Kentaro Horiguchi,Yoshinori Higuchi,Atsushi Onodera,Takashi Miki,Tomoaki Tanaka

iScience 27:111068 PubMed39483146

2024

Deciphering craniopharyngioma subtypes: Single-cell analysis of tumor microenvironment and immune networks.

Applications

Unspecified application

Species

Unspecified reactive species

Tatsuma Matsuda,Takashi Kono,Yuki Taki,Ikki Sakuma,Masanori Fujimoto,Naoko Hashimoto,Eiryo Kawakami,Noriaki Fukuhara,Hiroshi Nishioka,Naoko Inoshita,Shozo Yamada,Yasuhiro Nakamura,Kentaro Horiguchi,Takashi Miki,Yoshinori Higuchi,Tomoaki Tanaka

Cancer cell international 22:352 PubMed36376957

2022

Establishment of anti-DKK3 peptide for the cancer control in head and neck squamous cell carcinoma (HNSCC).

Applications

Unspecified application

Species

Unspecified reactive species

Naoki Katase,Shin-Ichiro Nishimatsu,Akira Yamauchi,Shinji Okano,Shuichi Fujita

Cancer research communications 2:772-783 PubMed36923312

2022

Suppressed Cellular Senescence Mediated by T-box3 in Aged Gastric Epithelial Cells may Contribute to Aging-related Carcinogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Akio Takeuchi,Naoki Asano,Akira Imatani,Masashi Saito,Xiaoyi Jin,Masahiro Saito,Takeshi Kanno,Waku Hatta,Kaname Uno,Tomoyuki Koike,Atsushi Masamune

Oral diseases 29:3193-3204 PubMed35708905

2022

DKK3/CKAP4 axis is associated with advanced stage and poorer prognosis in oral cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Naoki Katase,Kodai Kudo,Kazuhiro Ogawa,Yae Sakamoto,Shin-Ichiro Nishimatsu,Akira Yamauchi,Shuichi Fujita

Journal of neuroinflammation 19:129 PubMed35658977

2022

DKK3 ameliorates neuropathic pain via inhibiting ASK-1/JNK/p-38-mediated microglia polarization and neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Long-Qing Zhang,Shao-Jie Gao,Jia Sun,Dan-Yang Li,Jia-Yi Wu,Fan-He Song,Dai-Qiang Liu,Ya-Qun Zhou,Wei Mei

International journal of molecular medicine 49: PubMed35137918

2022

Dickkopf‑3 and β‑catenin play opposite roles in the Wnt/β‑catenin pathway during the abnormal subchondral bone formation of human knee osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Xuegang Liang,Qunhua Jin,Xiaochun Yang,Wenhui Jiang

Journal of cellular and molecular medicine 25:1583-1600 PubMed33462997

2021

Protein arginine methyltransferase 5 (PRMT5) activates WNT/β-catenin signalling in breast cancer cells via epigenetic silencing of DKK1 and DKK3.

Applications

Unspecified application

Species

Unspecified reactive species

Harshita Shailesh,Kodappully S Siveen,Saïd Sif

Cancer cell international 20:474 PubMed33013201

2020

Long noncoding RNA LINC00261 suppresses prostate cancer tumorigenesis through upregulation of GATA6-mediated DKK3.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Li,Hai Li,Xin Wei

Journal of neuroinflammation 17:130 PubMed32331523

2020

DKK3 attenuates JNK and AP-1 induced inflammation via Kremen-1 and DVL-1 in mice following intracerebral hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Xu,Derek Nowrangi,Hui Liang,Tian Wang,Lingyan Yu,Tai Lu,Zhengyang Lu,John H Zhang,Benyan Luo,Jiping Tang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Dkk3 antibody [EPR15611]

Immunocytochemistry/ Immunofluorescence - Anti-Dkk3 antibody [EPR15611] (AB186409)

Immunoprecipitation - Anti-Dkk3 antibody [EPR15611] (AB186409)

Western blot - Anti-Dkk3 antibody [EPR15611] (AB186409)

Western blot - Anti-Dkk3 antibody [EPR15611] (AB186409)

Western blot - Anti-Dkk3 antibody [EPR15611] (AB186409)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (18)

Product promise