Skip to main content

anti-Dkk3 antibody [EPR21736-265] ab244784 is a carrier free rabbit monoclonal antibody that is used in Dkk3 sELISA. Suitable for human samples.

- Create your own immunoassay with sensitive, specific matched antibody pairs
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests

Be the first to review this product! Submit a review

Images

Sandwich ELISA - Anti-Dkk3 antibody [EPR21736-265] - BSA and Azide free (Capture) (AB244784), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Expected

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity).

Alternative names

REIC, UNQ258/PRO295, DKK3, Dickkopf-related protein 3, Dickkopf-3, Dkk-3, hDkk-3

Recommended products

anti-Dkk3 antibody [EPR21736-265] ab244784 is a carrier free rabbit monoclonal antibody that is used in Dkk3 sELISA. Suitable for human samples.

- Create your own immunoassay with sensitive, specific matched antibody pairs
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR21736-265
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab244784 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human DKK3. The recommended pair for sandwich ELISA is:
Capture: ab244784, Human DKK3 Capture Antibody (unconjugated)
Detector: Anti-Dkk3 antibody [EPR21736-257] - BSA and Azide free (Detector) ab245057, Human DKK3 Detector Antibody (unconjugated)
The reference range value is 156 - 10000 pg/ml.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Anti-Dkk3 antibody [EPR21736-265] - BSA and Azide free (Capture) (ab244784), expandable thumbnail

    Sandwich ELISA - Anti-Dkk3 antibody [EPR21736-265] - BSA and Azide free (Capture) (ab244784)

    Representative standard curve from corresponding SimpleStep ELISA® Kit (Human DKK3 ELISA Kit ab236710).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com