Rabbit Recombinant Monoclonal DKK4 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-DKK4 antibody [EPR20956-132] - BSA and Azide free (Capture) (AB259537), expandable thumbnail](https://content.abcam.com/products/images/dkk4-antibody-epr20956-132-bsa-and-azide-free-capture-ab259537--sandwich-elisa-img55821.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-DKK4 antibody [EPR20956-132] - BSA and Azide free (Capture) (AB259537)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity).
Alternative names
Dickkopf-related protein 4, Dickkopf-4, Dkk-4, hDkk-4, DKK4
Recommended products
Rabbit Recombinant Monoclonal DKK4 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20956-132
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab259537 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human DKK4. The recommended pair for sandwich ELISA is:
Capture: ab259537, Human DKK4 Capture Antibody (unconjugated)
Detector: Anti-DKK4 antibody [EPR20956-28] - BSA and Azide free (Detector) ab259538, Human DKK4 Detector Antibody (unconjugated)
The reference range value is 21.88 - 1400 pg/ml.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Dickkopf-related protein 4 (DKK4) acts as an inhibitor of the Wnt signaling pathway which affects cell fate proliferation and migration. Known aliases for DKK4 include DKK-4 and DKK-4 protein. It possesses a molecular mass of around 27 kDa. DKK4 mainly expresses in the skin gastrointestinal tract and placenta indicating roles in these tissues and systems. Its presence suggests involvement in processes related to epithelial and stromal interactions.
- Biological function summary
DKK4 participates in the regulation of embryonic development and tissue homeostasis. It functions as a part of the larger Dickkopf protein family which modulates Wnt signaling by binding to LRP5/6 co-receptors. Through this interaction DKK4 manages the balance between cell growth and apoptosis important in both early development and adult tissue maintenance. DKK4 presence is often necessary to modulate signals that govern differentiation and proliferation in complex tissue systems.
- Pathways
DKK4 predominantly interacts within the Wnt signaling pathway. This pathway is critical for regulating gene expression involved in stem cell maintenance and differentiation. DKK4 acts by interacting with proteins such as LRP6 and Kremen thereby inhibiting Wnt-induced processes. Through modulation of these factors DKK4 ensures the fine-tuning of signaling cascades necessary for maintaining cellular equilibrium under physiological conditions.
- Associated diseases and disorders
Research associates DKK4 with malignant melanoma and colorectal cancer. These associations arise due to DKK4's role in modulating Wnt signaling which can drive oncogenic processes if dysregulated. In melanoma DKK4 can affect tumor cell proliferation by interacting with proteins like beta-catenin. In colorectal cancer abnormal expression of DKK4 might disrupt normal signaling balance further implicating it in tumor growth and progression. These connections highlight DKK4's potential as both a biomarker and a therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-DKK4 antibody [EPR20956-132] - BSA and Azide free (Capture) (ab259537), expandable thumbnail](https://content.abcam.com/products/images/dkk4-antibody-epr20956-132-bsa-and-azide-free-capture-ab259537--sandwich-elisa-img55821.jpg "Sandwich ELISA - Anti-DKK4 antibody [EPR20956-132] - BSA and Azide free (Capture) (ab259537)")
Sandwich ELISA - Anti-DKK4 antibody [EPR20956-132] - BSA and Azide free (Capture) (ab259537)
Representative standard curve from corresponding SimpleStep ELISA® Kit (Human DKK4 ELISA Kit ab243690).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com