Anti-DKKL1 antibody [EPR25721-68] (ab316308)

Rabbit Recombinant Monoclonal DKKL1 antibody. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P, WB, mIHC and reacts with Transfected cell line - Human, Human, Mouse, Rat samples.

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Images

Multiplex immunohistochemistry - Anti-DKKL1 antibody [EPR25721-68] (AB316308), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	Flow Cyt (Intra)	IHC-P	WB	IP	mIHC
Human	Expected	Expected	Tested	Tested	Not recommended	Tested
Mouse	Expected	Expected	Tested	Tested	Not recommended	Expected
Rat	Expected	Expected	Tested	Not recommended	Not recommended	Expected
Transfected cell line - Human	Tested	Tested	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell line - Human	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell line - Human	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell line - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Transfected cell line - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Transfected cell line - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/2000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell line - Human	Dilution info -	Notes -

Target data

See full target information DKKL1

Function

Involved in fertilization by facilitating sperm penetration of the zona pellucida. May promote spermatocyte apoptosis, thereby limiting sperm production. In adults, may reduce testosterone synthesis in Leydig cells. Is not essential either for development or fertility.

Alternative names

SGY1, UNQ735/PRO1429, DKKL1, Dickkopf-like protein 1, Cancer/testis antigen 34, Protein soggy-1, CT34, SGY-1

Rabbit Recombinant Monoclonal DKKL1 antibody. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P, WB, mIHC and reacts with Transfected cell line - Human, Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR25721-68
Purification technique: Affinity purification Protein A
Specificity: Unsuitable for rat WB.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

DKKL1 also known as Dickkopf-like protein 1 is a protein with a mass of approximately 32 kDa. The protein is expressed in several tissues including the testes and brain. DKKL1 belongs to the Dickkopf family which is known for involvement in regulating cellular signaling pathways. Mechanically DKKL1 functions as a modulator in extracellular environments where it can interact with other components of the signaling pathways.

Biological function summary

DKKL1 has roles in cellular differentiation and development processes. While it does not form part of a larger protein complex its function is important in processes that dictate how cells grow and mature. In particular DKKL1 influences key developmental stages possibly affecting the balance between cell proliferation and differentiation.

Pathways

DKKL1 plays a role in the Wnt signaling pathway a critical pathway for cell-to-cell communication during embryonic development and cancer progression. DKKL1's role in modulating this pathway involves interaction with other proteins such as components of the Frizzled receptor family which help transmit signaling from the cell surface to the nucleus dictating cellular responses.

Associated diseases and disorders

DKKL1 has connections with disorders related to abnormal development and cancer. For example aberrant expression of DKKL1 has been implicated in testicular germ cell tumors where it interacts with proteins such as beta-catenin an important mediator in the Wnt signaling pathway. Alterations in DKKL1 expression can contribute to disrupted signaling potentially leading to tumorigenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

Multiplex immunohistochemistry - Anti-DKKL1 antibody [EPR25721-68] (ab316308)
Multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded Human testis tissue sections labeling DKKL1 with ab316308 at a 1/2000 dilutions (B), Wilms Tumor Protein with Anti-Wilms Tumor Protein antibody [EPR23963-116] - BSA and Azide free ab275970 at a 1/1200 dilutions (C), and SAGE1 with Anti-SAGE1 antibody [EPR21747] ab233388 at a 1/250 dilutions (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody.

Panel A: merged staining of anti-DKKL1 (red; Opal™ 570), anti-Wilms Tumor Protein (green; Opal™ 520) and anti-SAGE1 (gray; Opal™ 690) on human testis.

Panel B: anti-DKKL1 staining mature sperm cells in human testis.

Panel C: anti-Wilms Tumor Protein staining sertoli cells in human testis.

Panel D: anti-SAGE1 staining spermatogonia in human testis.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. The section was incubated in three rounds of staining: in the order of ab316308, Anti-Wilms Tumor Protein antibody [EPR23963-116] - BSA and Azide free ab275970, and Anti-SAGE1 antibody [EPR21747] ab233388 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
DAPI was used as a nuclear counterstain.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Flow Cytometry (Intracellular) - Anti-DKKL1 antibody [EPR25721-68] (ab316308)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T cells transfected with a human DKKL1 expression vector containing a myc-His-tag® (Middle) / 293T cells transfected with an empty expression vector containing a myc-His-tag® (Right) cells labelling DKKL1 with ab316308 at 1/500 dilution (0.1ug)/ Middle and Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Cells are co-stained with Myc tag conjugated to Alexa Fluor®647.
Immunocytochemistry/ Immunofluorescence - Anti-DKKL1 antibody [EPR25721-68] (ab316308)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling DKKL1 with ab316308 at 1/100 (5.14 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing positive staining in 293T cells transfected with a human DKKL1 expression vector containing a myc-His-tag®. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-Myc tag antibody [9E10] ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain at 1/100 (5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKKL1 antibody [EPR25721-68] (ab316308)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling DKKL1 with ab316308 at 1/2000 (0.257 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on rat cerebrum (PMID: 15892050).

The section was incubated with ab316308 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKKL1 antibody [EPR25721-68] (ab316308)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling DKKL1 with ab316308 at 1/2000 (0.257 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse cerebrum (PMID: 15892050).

The section was incubated with ab316308 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKKL1 antibody [EPR25721-68] (ab316308)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling DKKL1 with ab316308 at 1/2000 (0.257 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human cerebrum.

The section was incubated with ab316308for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKKL1 antibody [EPR25721-68] (ab316308)
Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling DKKL1 with ab316308 at 1/2000 (0.257 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat testis.

The section was incubated with ab316308 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKKL1 antibody [EPR25721-68] (ab316308)
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling DKKL1 with ab316308 at 1/2000 (0.257 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human testis (PMID: 27699219).

The section was incubated with ab316308 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKKL1 antibody [EPR25721-68] (ab316308)
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling DKKL1 with ab316308 at 1/2000 (0.257 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse testis (PMID: 22817830).

The section was incubated with ab316308 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Western blot - Anti-DKKL1 antibody [EPR25721-68] (ab316308)
Negative control: brain (PMID: 15892050).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-DKKL1 antibody [EPR25721-68] (ab316308) at 1/1000 dilution
Lane 1: Mouse testis tissue lysate at 40 µg with 5% NFDM/TBST
Lane 2: Mouse brain tissue lysate at 40 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 32 kDa, 36 kDa
Exposure time: 180s
Western blot - Anti-DKKL1 antibody [EPR25721-68] (ab316308)
Negative control: lung (PMID: 15892050), brain (PMID: 15892050).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-DKKL1 antibody [EPR25721-68] (ab316308) at 1/1000 dilution
Lane 1: Human testis tissue lysate at 40 µg with 5% NFDM/TBST
Lane 2: Human lung tissue lysate at 40 µg with 5% NFDM/TBST
Lane 3: Human brain tissue lysate at 40 µg with 5% NFDM/TBST
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 32 kDa
Exposure time: 180s