AB157468

Anti-DLC-A antibody [EPR11240]

RabMAb
Recombinant
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(7 Publications)

Rabbit Recombinant Monoclonal DLC-A antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 7 publications.

View Alternative Names

DNCLI1, DYNC1LI1, Cytoplasmic dynein 1 light intermediate chain 1, LIC1, Dynein light chain A, DLC-A, DLIC-1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLC-A antibody [EPR11240] (AB157468)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLC-A antibody [EPR11240] (AB157468)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling DLC-A with ab157468 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-DLC-A antibody [EPR11240] (AB157468)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DLC-A antibody [EPR11240] (AB157468)

Immunofluorescent analysis of K562 cells labeling DLC-A with ab157468 at 1/100 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLC-A antibody [EPR11240] (AB157468)

Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling DLC-A with ab157468 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-DLC-A antibody [EPR11240] (AB157468)

All lanes:

Western blot - Anti-DLC-A antibody [EPR11240] (ab157468) at 1/1000 dilution

All lanes:

immunoprecipitation pellet from fetal brain lysate

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Western blot - Anti-DLC-A antibody [EPR11240] (AB157468)

All lanes:

Western blot - Anti-DLC-A antibody [EPR11240] (ab157468) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Fetal brain tissue lysate at 10 µg

Lane 4:

Fetal heart tissue lysate at 10 µg

Lane 5:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 57 kDa

false

Carrier free

Anti-DLC-A antibody [EPR11240] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11240

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DLC-A also known as Deleted in Liver Cancer-1 or ARHGEF5 is a Rho guanine nucleotide exchange factor with a mass of approximately 205 kDa. This protein plays a role in cellular processes by activating members of the Rho GTPase family which are key regulators of the cytoskeleton. DLC-A is expressed in various tissues including liver kidney and heart suggesting its broad implication in cellular functions across different organ systems.

Biological function summary

DLC-A acts as a guanine nucleotide exchange factor facilitating the exchange of GDP for GTP on Rho family GTPases. This activity leads to the activation of these GTPases promoting cytoskeletal rearrangements and influencing cell shape motility and growth. DLC-A integrates into large protein complexes interacting with various other signaling molecules that modulate its activity in different cellular contexts highlighting its broad involvement in cell dynamics.

Pathways

Many cellular transformations and regulatory systems involve DLC-A. DLC-A participates in the Rho signaling pathway important for actin cytoskeleton dynamics. It also interacts with the MAPK/ERK pathway which influences cell division and survival. In these pathways DLC-A connects with proteins like RhoA and ERK1/2 showing its pivotal role in signal transduction and cellular responses to external signals.

Genomic alterations and expression changes in DLC-A have links to cancer progression especially in liver cancer. Researchers have found that altered DLC-A expression affects tumor formation and metastasis through its action in cytoskeletal remodeling and cell movement. Furthermore DLC-A relates to cardiovascular disorders due to its significant effects on vascular cell function. In these contexts DLC-A interacts with other proteins like PI3K and Akt which are involved in cell proliferation and survival emphasizing its role in disease etiology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in binding dynein to membranous organelles or chromosomes. Probably involved in the microtubule-dependent transport of pericentrin. Is required for progress through the spindle assembly checkpoint. The phosphorylated form appears to be involved in the selective removal of MAD1L1 and MAD1L2 but not BUB1B from kinetochores. Forms a functional Rab11/RAB11FIP3/dynein complex onto endosomal membrane that regulates the movement of peripheral sorting endosomes (SE) along microtubule tracks toward the microtubule organizing center/centrosome, generating the endosomal recycling compartment (ERC) (PubMed : 20026645).

See full target information DYNC1LI1

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

PLoS genetics 18:e1010232 PubMed35727824

2022

Dync1li1 is required for the survival of mammalian cochlear hair cells by regulating the transportation of autophagosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Zhang,Shasha Zhang,Han Zhou,Xiangyu Ma,Leilei Wu,Mengyao Tian,Siyu Li,Xiaoyun Qian,Xia Gao,Renjie Chai

The Journal of cell biology 220: PubMed34014261

2021

Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Sydney E Cason,Peter J Carman,Claire Van Duyne,Juliet Goldsmith,Roberto Dominguez,Erika L F Holzbaur

The EMBO journal :e103661 PubMed33215754

2020

Distinct retrograde microtubule motor sets drive early and late endosome transport.

Applications

Unspecified application

Species

Unspecified reactive species

Giulia Villari,Chiara Enrico Bena,Marco Del Giudice,Noemi Gioelli,Chiara Sandri,Chiara Camillo,Alessandra Fiorio Pla,Carla Bosia,Guido Serini

Scientific reports 10:11806 PubMed32678227

2020

Cdc42 regulates cell polarization and contractile actomyosin rings during terminal differentiation of human erythroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Kumi Ubukawa,Tatsufumi Goto,Ken Asanuma,Yumi Sasaki,Yong-Mei Guo,Isuzu Kobayashi,Kenichi Sawada,Hideki Wakui,Naoto Takahashi

Oncogene 38:1151-1165 PubMed30250299

2018

Cytoplasmic dynein regulates the subcellular localization of sphingosine kinase 2 to elicit tumor-suppressive functions in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Heidi A Neubauer,Melinda N Tea,Julia R Zebol,Briony L Gliddon,Cassandra Stefanidis,Paul A B Moretti,Melissa R Pitman,Maurizio Costabile,Jasreen Kular,Brett W Stringer,Bryan W Day,Michael S Samuel,Claudine S Bonder,Jason A Powell,Stuart M Pitson

Cancer immunology research 6:1057-1068 PubMed30018046

2018

Mechanisms by Which Dendritic Cells Present Tumor Microparticle Antigens to CD8 T Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jingwei Ma,Keke Wei,Huafeng Zhang,Ke Tang,Fei Li,Tianzhen Zhang,Junwei Liu,Pingwei Xu,Yuandong Yu,Weiwei Sun,LiYan Zhu,Jie Chen,Li Zhou,Xiaoyu Liang,Jiadi Lv,Roland Fiskesund,Yuying Liu,Bo Huang

Proceedings of the National Academy of Sciences of 113:E2955-64 PubMed27170189

2016

PDK1-Akt pathway regulates radial neuronal migration and microtubules in the developing mouse neocortex.

Applications

Unspecified application

Species

Unspecified reactive species

Yasuhiro Itoh,Maiko Higuchi,Koji Oishi,Yusuke Kishi,Tomohiko Okazaki,Hiroshi Sakai,Takaki Miyata,Kazunori Nakajima,Yukiko Gotoh

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DLC-A antibody [EPR11240]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLC-A antibody [EPR11240] (AB157468)

Immunocytochemistry/ Immunofluorescence - Anti-DLC-A antibody [EPR11240] (AB157468)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLC-A antibody [EPR11240] (AB157468)

Western blot - Anti-DLC-A antibody [EPR11240] (AB157468)

Western blot - Anti-DLC-A antibody [EPR11240] (AB157468)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise