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AB126257

Anti-DLC1 antibody

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(6 Publications)

Rabbit Polyclonal DLC1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DLC1 aa 1250 to C-terminus.

View Alternative Names

ARHGAP7, KIAA1723, STARD12, DLC1, Rho GTPase-activating protein 7, Deleted in liver cancer 1 protein, HP protein, Rho-type GTPase-activating protein 7, START domain-containing protein 12, StAR-related lipid transfer protein 12, DLC-1, StARD12

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLC1 antibody (AB126257)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLC1 antibody (AB126257)

ab126257, at 1/500, staining DLC1 in Human Breast ca tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P).

Western blot - Anti-DLC1 antibody (AB126257)
  • WB

Unknown

Western blot - Anti-DLC1 antibody (AB126257)

Gel concentration : 5%

All lanes:

Western blot - Anti-DLC1 antibody (ab126257) at 1/1000 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 125 kDa,146 kDa,153 kDa,166 kDa,171 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human DLC1 aa 1250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96QB1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DLC1 also known as Deleted in Liver Cancer 1 is a tumor suppressor protein with a mass of approximately 123 kilodaltons. It functions mechanically as a Rho GTPase-activating protein (RhoGAP) regulating the activity of Rho family GTPases. DLC1 is expressed in various tissues with significant expression in the liver lung and kidney. The protein helps maintain cytoskeletal integrity and cell stability by modulating actin dynamics and cellular contractility.
Biological function summary

The protein DLC1 plays an important role in cell migration proliferation and adhesion. It often forms part of a multi-protein complex with tensin proteins which helps link it to the actin cytoskeleton. Through its GAP activity DLC1 negatively regulates RhoA a small GTPase. This regulation affects cell shape and movement by controlling focal adhesion turnover and stress fiber formation essential processes in maintaining normal cellular architecture.

Pathways

DLC1 impacts cellular signaling related to the cell cycle and apoptosis. It is heavily involved in the regulation of the Rho/Rac signaling pathway which is critical for actin cytoskeleton organization and cellular dynamics. DLC1 interacts with proteins such as RhoA and Cdc42 within these pathways facilitating signal transduction that affects cellular responses to external stimuli and internal checkpoints ensuring proper cell division and programmed cell death.

DLC1 is closely related to various cancers including liver and breast cancer. Its tumor suppressor function is often lost or reduced in these conditions leading to unchecked cellular proliferation and metastasis. In the context of liver cancer DLC1 interacts with proteins such as PTEN influencing tumor growth and progression. Aberrations in DLC1 expression or activity can also correlate with other disorders involving disrupted cell signaling and cytoskeletal abnormalities.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a GTPase-activating protein for the small GTPases RHOA, RHOB, RHOC and CDC42, terminating their downstream signaling. This induces morphological changes and detachment through cytoskeletal reorganization, playing a critical role in biological processes such as cell migration and proliferation. Also functions in vivo as an activator of the phospholipase PLCD1. Active DLC1 increases cell migration velocity but reduces directionality. Required for growth factor-induced epithelial cell migration; in resting cells, interacts with TNS3 while PTEN interacts with the p85 regulatory subunit of the PI3K kinase complex but growth factor stimulation induces phosphorylation of TNS3 and PTEN, causing them to change their binding preference so that PTEN interacts with DLC1 and TNS3 interacts with p85 (PubMed : 26166433). The PTEN-DLC1 complex translocates to the posterior of migrating cells to activate RHOA while the TNS3-p85 complex translocates to the leading edge of migrating cells to promote RAC1 activation (PubMed : 26166433).
See full target information DLC1
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Aging 15:9809-9821 PubMed37737712

2023

The oncogenic miR-429 promotes triple-negative breast cancer progression by degrading DLC1.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Li,Xue Meng,Yuqing Luo,Shuai Luo,Jin Li,Jiafei Zeng,Xiang Huang,Jinjing Wang

Neoplasma 69:1165-1174 PubMed35951458

2022

NBR2/miR-561-5p/DLC1 axis inhibited the development of multiple myeloma by activating the AMPK/mTOR pathway to repress glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Chang-Sheng Wang,Xiao-Bo Zhang,Xi-Tian Zhu,Rong-Sheng Chen

Technology in cancer research & treatment 20:1533033821990036 PubMed33754907

2021

MIR-301b-3p Promotes Lung Adenocarcinoma Cell Proliferation, Migration and Invasion by Targeting DLC1.

Applications

Unspecified application

Species

Unspecified reactive species

Haitao Liu,Xingjie Ma,Niu Niu,Junjie Zhao,Chao Lu,Fan Yang,Weibo Qi

Molecular medicine reports 19:4881-4889 PubMed31059015

2019

High expression of DLC family proteins predicts better prognosis and inhibits tumor progression in NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Li Sun,Jing Sun,Jun-Ding Song

Oncotarget 8:94834-94849 PubMed29212270

2017

Exosomal miR-141-3p regulates osteoblast activity to promote the osteoblastic metastasis of prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Ye,Su-Liang Li,Yue-Yun Ma,Yan-Jun Diao,Liu Yang,Ming-Quan Su,Zhuo Li,Yang Ji,Juan Wang,Lin Lei,Wei-Xiao Fan,La-Xiu Li,Yi Xu,Xiao-Ke Hao

Oncology letters 12:2163-2168 PubMed27602157

2016

miR-429 promotes the proliferation of non-small cell lung cancer cells via targeting DLC-1.

Applications

WB

Species

Human

Peng Xiao,Wenliang Liu,Hui Zhou
View all publications
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Product promise

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