Rabbit Polyclonal DLC1 antibody. C-terminal. Suitable for WB and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DLC1.
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- Scientific reports 8:88752018Notochordal cell matrix as a bioactive lubricant for the osteoarthritic joint.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS A H de Vries et. al.PubMed 29891965
- Tissue engineering. Part A 25:830-8412018Notochordal Cell Matrix As a Therapeutic Agent for Intervertebral Disc Regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStefan de Vries,Marina van Doeselaar,Björn Meij,Marianna Tryfonidou,Keita ItoPubMed 29739272
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human DLC1. The exact immunogen used to generate this antibody is proprietary information. Database link Q96QB1
Reactivity data
Select an application| WB | |
|---|---|
| Mouse | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Functions as a GTPase-activating protein for the small GTPases RHOA, RHOB, RHOC and CDC42, terminating their downstream signaling. This induces morphological changes and detachment through cytoskeletal reorganization, playing a critical role in biological processes such as cell migration and proliferation. Also functions in vivo as an activator of the phospholipase PLCD1. Active DLC1 increases cell migration velocity but reduces directionality. Required for growth factor-induced epithelial cell migration; in resting cells, interacts with TNS3 while PTEN interacts with the p85 regulatory subunit of the PI3K kinase complex but growth factor stimulation induces phosphorylation of TNS3 and PTEN, causing them to change their binding preference so that PTEN interacts with DLC1 and TNS3 interacts with p85 (PubMed:26166433). The PTEN-DLC1 complex translocates to the posterior of migrating cells to activate RHOA while the TNS3-p85 complex translocates to the leading edge of migrating cells to promote RAC1 activation (PubMed:26166433).
Alternative names
ARHGAP7, KIAA1723, STARD12, DLC1, Rho GTPase-activating protein 7, Deleted in liver cancer 1 protein, HP protein, Rho-type GTPase-activating protein 7, START domain-containing protein 12, StAR-related lipid transfer protein 12, DLC-1, StARD12
Recommended products
Rabbit Polyclonal DLC1 antibody. C-terminal. Suitable for WB and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DLC1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human DLC1. The exact immunogen used to generate this antibody is proprietary information. Database link Q96QB1
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DLC1 also known as Deleted in Liver Cancer 1 is a tumor suppressor protein with a mass of approximately 123 kilodaltons. It functions mechanically as a Rho GTPase-activating protein (RhoGAP) regulating the activity of Rho family GTPases. DLC1 is expressed in various tissues with significant expression in the liver lung and kidney. The protein helps maintain cytoskeletal integrity and cell stability by modulating actin dynamics and cellular contractility.
- Biological function summary
The protein DLC1 plays an important role in cell migration proliferation and adhesion. It often forms part of a multi-protein complex with tensin proteins which helps link it to the actin cytoskeleton. Through its GAP activity DLC1 negatively regulates RhoA a small GTPase. This regulation affects cell shape and movement by controlling focal adhesion turnover and stress fiber formation essential processes in maintaining normal cellular architecture.
- Pathways
DLC1 impacts cellular signaling related to the cell cycle and apoptosis. It is heavily involved in the regulation of the Rho/Rac signaling pathway which is critical for actin cytoskeleton organization and cellular dynamics. DLC1 interacts with proteins such as RhoA and Cdc42 within these pathways facilitating signal transduction that affects cellular responses to external stimuli and internal checkpoints ensuring proper cell division and programmed cell death.
- Associated diseases and disorders
DLC1 is closely related to various cancers including liver and breast cancer. Its tumor suppressor function is often lost or reduced in these conditions leading to unchecked cellular proliferation and metastasis. In the context of liver cancer DLC1 interacts with proteins such as PTEN influencing tumor growth and progression. Aberrations in DLC1 expression or activity can also correlate with other disorders involving disrupted cell signaling and cytoskeletal abnormalities.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-DLC1 antibody - C-terminal (ab180697)
All lanes: Western blot - Anti-DLC1 antibody - C-terminal (ab180697) at 1/500 dilution
All lanes: Mouse lung extract
Predicted band size: 171 kDa
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