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AB325661

Anti-DLG4 antibody [K28/43] - Chicken IgY (Chimeric)

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Chicken Monoclonal PSD95 antibody. Suitable for IHC-P, WB and reacts with Rat samples.

View Alternative Names

Dlgh4, Psd95, Dlg4, Disks large homolog 4, Postsynaptic density protein 95, Synapse-associated protein 90, PSD-95, SAP-90, SAP90

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLG4 antibody [K28/43] - Chicken IgY (Chimeric) (AB325661)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLG4 antibody [K28/43] - Chicken IgY (Chimeric) (AB325661)

Formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of DLG4. The sections were stained with ab325661 at 1 : 500 dilution (green). The stained sections were mounted with Fluoroshield with DAPI and DABCO mounting medium. DAPI nuclear stain (blue) shows cell nuclei. The antibody specifically stains the excitatory synapses in the granular layer of the cerebellum. Magnification 400X.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLG4 antibody [K28/43] - Chicken IgY (Chimeric) (AB325661)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLG4 antibody [K28/43] - Chicken IgY (Chimeric) (AB325661)

Formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of DLG4. The sections were stained with ab325661 at 1 : 500 dilution (green). The stained sections were mounted with Fluoroshield with DAPI and DABCO mounting medium. DAPI nuclear stain (blue) shows cell nuclei. The antibody specifically stains the excitatory synapses in the granular layer of the cerebellum. Magnification 100X.

Western blot - Anti-DLG4 antibody [K28/43] - Chicken IgY (Chimeric) (AB325661)
  • WB

Supplier Data

Western blot - Anti-DLG4 antibody [K28/43] - Chicken IgY (Chimeric) (AB325661)

Western blotting of rat brain homogenate with an anti-PSD-95 mouse Mab and ab325661 antibodies (2 µg/ml). The mouse Mab and chicken Mab were detected with anti-mouse-HRP and anti-chicken-HRP, respectively. ab325661 recognizes endogenous DLG4 with similar sensitivity as the anti-PSD-95 mouse Mab in the rat brain homogenate at 95-110 kDa. PSD-95 is an alternative name for DLG4.

All lanes:

Western blot - Anti-DLG4 antibody [K28/43] - Chicken IgY (Chimeric) (ab325661) at 2 µg/mL

All lanes:

rat brain homogenate

Secondary

All lanes:

anti-chicken-HRP

Observed band size: 95-110 kDa

false

Key facts

Host species

Chicken

Clonality

Monoclonal

Clone number

K28/43

Isotype

IgY

Carrier free

No

Reacts with

Rat

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

No cross-reactivity against recombinant Chapsyn-110, SAP97 or SAP102.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone K28/43 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone K28/43 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Postsynaptic scaffolding protein that plays a critical role in synaptogenesis and synaptic plasticity by providing a platform for the postsynaptic clustering of crucial synaptic proteins (PubMed : 15317815, PubMed : 15358863, PubMed : 19596852, PubMed : 20628354, PubMed : 23300088, PubMed : 26679993). Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B (By similarity). Also regulates AMPA-type glutamate receptor (AMPAR) immobilization at postsynaptic density keeping the channels in an activated state in the presence of glutamate and preventing synaptic depression (PubMed : 19596852). Under basal conditions, cooperates with FYN to stabilize palmitoyltransferase ZDHHC5 at the synaptic membrane through FYN-mediated phosphorylation of ZDHHC5 and its subsequent inhibition of association with endocytic proteins (By similarity).
See full target information Dlg4
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Product promise

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