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AB86783

Anti-DLG5 antibody

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(4 Publications)

Rabbit Polyclonal DLG5 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human DLG5 aa 1300-1400.

View Alternative Names

KIAA0583, PDLG, DLG5, Disks large homolog 5, Discs large protein P-dlg, Placenta and prostate DLG

2 Images
Western blot - Anti-DLG5 antibody (AB86783)
  • WB

Unknown

Western blot - Anti-DLG5 antibody (AB86783)

All lanes:

Western blot - Anti-DLG5 antibody (ab86783) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 214 kDa

true

Exposure time: 3min

Immunoprecipitation - Anti-DLG5 antibody (AB86783)
  • IP

Unknown

Immunoprecipitation - Anti-DLG5 antibody (AB86783)

Detection of DLG5 by Western Blot of Immunprecipitate.
ab86783, at 1 µg/ml, staining DLG5 in HeLa whole cell lysate immunoprecipitated using ab86783 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-DLG5 antibody (ab86783)

Predicted band size: 214 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human DLG5 aa 1300-1400. The exact immunogen used to generate this antibody is proprietary information.

Q8TDM6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab86783 was affinity purified using an epitope specific to DLG5 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein Discs Large MAGUK Scaffold Protein 5 known as DLG5 functions as a scaffolding protein. It is part of the membrane-associated guanylate kinase (MAGUK) family and has an approximate mass of 220 kDa. DLG5 contains multiple domains including PDZ SH3 and GK which assist in protein-protein interactions. This protein is expressed in various tissues including the heart brain and skeletal muscle implying its widespread importance in different physiological contexts.
Biological function summary

The DLG5 protein plays a role in cell polarity and tissue development. It associates with components of the cytoskeleton and participates in maintaining the structural integrity of cells. DLG5 is part of a larger protein complex that regulates the signaling pathways important for cell differentiation and proliferation. Its interactions are essential for establishing cellular junctions that uphold cell adhesion and tissue architecture.

Pathways

The DLG5 protein is an integral part of the Hippo and Wnt signaling pathways. These pathways are critical in regulating cell growth and apoptosis. In the Wnt pathway DLG5 interacts with other proteins such as beta-catenin to influence gene transcription that steers cell cycle progression. In the Hippo pathway DLG5 interfaces with the LATS1/2 kinases to maintain control over organ size by modulating cell proliferation and apoptosis.

The DLG5 protein shows a connection to inflammatory bowel disease and some cancers. Mutations or altered expression of DLG5 have been linked to Crohn's disease suggesting its role in intestinal barrier function. Additionally its involvement in cancer highlights a relationship with tumor suppressor pathways. Through these diseases the protein interacts with others like APC and TCF within pathways that change cell adhesion and proliferation potentially assisting tumor development or impacting inflammatory responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a regulator of the Hippo signaling pathway (PubMed : 28087714, PubMed : 28169360). Negatively regulates the Hippo signaling pathway by mediating the interaction of MARK3 with STK3/4, bringing them together to promote MARK3-dependent hyperphosphorylation and inactivation of STK3 kinase activity toward LATS1 (PubMed : 28087714). Positively regulates the Hippo signaling pathway by mediating the interaction of SCRIB with STK4/MST1 and LATS1 which is important for the activation of the Hippo signaling pathway. Involved in regulating cell proliferation, maintenance of epithelial polarity, epithelial-mesenchymal transition (EMT), cell migration and invasion (PubMed : 28169360). Plays an important role in dendritic spine formation and synaptogenesis in cortical neurons; regulates synaptogenesis by enhancing the cell surface localization of N-cadherin. Acts as a positive regulator of hedgehog (Hh) signaling pathway. Plays a critical role in the early point of the SMO activity cycle by interacting with SMO at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation (By similarity).
See full target information DLG5
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Discover oncology 16:1700 PubMed40993338

2025

The reduced DLG5 promotes doxorubicin resistance in breast cancer cells MCF-7 through regulating autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jingmin Che,Xusheng Wang,Bo Chen,Huxia Wang,Qing Feng,Jingying Sun,Cuixiang Xu,Zhangjun Song

BMC cancer 25:35 PubMed39780116

2025

Correlation analysis of DLG5 and PD-L1 expression in triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jingmin Che,Bo Chen,Xusheng Wang,Baoe Liu,Cuixiang Xu,Huxia Wang,Jingying Sun,Qing Feng,Xiangrong Zhao,Zhangjun Song

Nature communications 12:5315 PubMed34493720

2021

Proximity proteomics identifies PAK4 as a component of Afadin-Nectin junctions.

Applications

Unspecified application

Species

Unspecified reactive species

Yohendran Baskaran,Felicia Pei-Ling Tay,Elsa Yuen Wai Ng,Claire Lee Foon Swa,Sheena Wee,Jayantha Gunaratne,Edward Manser

Biotechnology and bioengineering 118:1649-1663 PubMed33463716

2021

Metabolic engineering of HEK293 cells to improve transient transfection and cell budding of HIV-1 virus-like particles.

Applications

Unspecified application

Species

Unspecified reactive species

Jesús Lavado-García,Andy Díaz-Maneh,Núria Canal-Paulí,Pol Pérez-Rubio,Francesc Gòdia,Laura Cervera
View all publications
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