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AB249441

Anti-DLGAP4 antibody [EPR10888] - BSA and Azide free

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Rabbit Recombinant Monoclonal DLGAP4 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat samples.

View Alternative Names

DAP4, KIAA0964, SAPAP4, DLGAP4, Disks large-associated protein 4, DAP-4, PSD-95/SAP90-binding protein 4, SAP90/PSD-95-associated protein 4, SAPAP-4

1 Images
Western blot - Anti-DLGAP4 antibody [EPR10888] - BSA and Azide free (AB249441)
  • WB

Unknown

Western blot - Anti-DLGAP4 antibody [EPR10888] - BSA and Azide free (AB249441)

This data was developed using ab168339, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-DLGAP4 antibody [EPR10888] (<a href='/en-us/products/primary-antibodies/dlgap4-antibody-epr10888-ab168339'>ab168339</a>) at 1/1000 dilution

Lane 1:

Mouse brain lysates at 10 µg

Lane 2:

Rat brain lysates at 10 µg

Predicted band size: 108 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10888

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab249441 is the carrier-free version of ab168339.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DLGAP4 also known as SAPAP4 or DAP4 plays a role as a scaffolding protein at the postsynaptic density of neurons where it interacts with other proteins to facilitate synaptic signaling. This protein has a molecular mass of approximately 107 kDa. Its expression is primarily found in the brain particularly in the cerebral cortex and the hippocampus which suggests a specific role in neuronal function and behavior regulation.
Biological function summary

DLGAP4 participates as part of a postsynaptic complex that includes members of the DLG (discs large) protein family. This association is important for organizing and maintaining the structure of synaptic connections. The protein contributes to regulating synapse organization and plasticity which are important for learning and memory processes. Additionally it mediates interactions between the postsynaptic density components and the actin cytoskeleton aiding in the structural stability of synapses.

Pathways

DLGAP4 acts within the glutamatergic signaling pathway where it influences the dynamics of glutamate receptors. It associates with proteins such as PSD-95 which are integral in coupling receptors and other signaling molecules. The involvement in this pathway highlights the role of DLGAP4 in modulating excitatory synaptic transmission and synaptic plasticity which are essential for cognitive functions.

Disruptions in DLGAP4 expression or function have been linked to neurological diseases like schizophrenia and autism spectrum disorder. Abnormalities in DLGAP4 may affect brain circuits responsible for these conditions. Within these contexts DLGAP4 interacts with other synaptic proteins such as SHANK3 which when mutated or dysfunctional contribute to the pathophysiology of these disorders. Understanding DLGAP4's interactions can help illuminate mechanisms underlying synaptic dysfunction in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a role in the molecular organization of synapses and neuronal cell signaling. Could be an adapter protein linking ion channel to the subsynaptic cytoskeleton. May induce enrichment of PSD-95/SAP90 at the plasma membrane.
See full target information DLGAP4
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com