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AB119930

Anti-DLK-1 antibody [3A10]

4

(2 Reviews)

|

(16 Publications)

Mouse Monoclonal DLK-1 antibody. Suitable for Flow Cyt, ELISA, WB, ICC/IF and reacts with Mouse, Human, Synthetic peptide, Recombinant full length protein samples. Cited in 16 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DLK1 aa 150-350.

View Alternative Names

DLK, DLK1, Protein delta homolog 1, DLK-1, pG2

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-DLK-1 antibody [3A10] (AB119930)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DLK-1 antibody [3A10] (AB119930)

Immunofluorescence analysis of U251 cells using ab119930 at 1/200 dilution (green). Blue : DRAQ5 fluorescent DNA dye. Red : Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow Cytometry - Anti-DLK-1 antibody [3A10] (AB119930)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-DLK-1 antibody [3A10] (AB119930)

Flow cytometric analysis of NIH3T3 cells using ab119930 at 1/200 dilution (green) and negative control (red).

ELISA - Anti-DLK-1 antibody [3A10] (AB119930)
  • ELISA

Unknown

ELISA - Anti-DLK-1 antibody [3A10] (AB119930)

ELISA using ab119930.

Western blot - Anti-DLK-1 antibody [3A10] (AB119930)
  • WB

Unknown

Western blot - Anti-DLK-1 antibody [3A10] (AB119930)

All lanes:

Western blot - Anti-DLK-1 antibody [3A10] (ab119930) at 1/500 dilution

All lanes:

Recombinant DLK-1 protein

Predicted band size: 21 kDa,25 kDa,31 kDa,37 kDa,39 kDa,41 kDa,68 kDa,84 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3A10

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Mouse, Human

Applications

ELISA, Flow Cyt, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human DLK1 aa 150-350. The exact immunogen used to generate this antibody is proprietary information.

P80370

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Secondary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant full length protein": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1/10000", "ELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Delta-like 1 homolog (DLK-1) also known as Pref-1 (preadipocyte factor 1) is a protein involved in a wide range of cellular processes. The mass of DLK-1 is approximately 65 kDa. This protein is expressed in various tissues including the liver brain and endocrine glands. DLK-1 has an important role in the regulation of cell differentiation and growth acting as a signal molecule that influences tissue development and regeneration.
Biological function summary

DLK-1 serves a function in the inhibition of adipocyte differentiation and the maintenance of preadipocyte phenotype. It does not form part of a complex but interacts with membrane-bound receptors to mediate signaling pathways that impact tissue development. Researchers study its role in embryonic development and its downregulation is a significant factor during the differentiation of mesenchymal cells. Due to these interactions DLK-1 has a pivotal involvement in processes governing tissue regeneration and growth control.

Pathways

Better understanding of DLK-1 involves examining its interaction in the Notch signaling pathway. This important pathway related to cell fate decisions features pathways with other proteins like Notch-1 and Notch-3. The relationship helps modulate the fate and proliferation of cells. DLK-1 also has associations with pathways involving phosphoinositide 3-kinase (PI3K) which influences cell growth and survival through related proteins such as AKT.

DLK-1 has shown associations with cancer and metabolic disorders. Overexpression of DLK-1 is linked to obesity due to its impact on preadipocyte differentiation. Additionally higher levels of DLK-1 can correlate with certain cancer progression where it acts alongside proteins like Notch-1 in promoting tumor cell growth. Understanding DLK-1's role in these contexts highlights the importance of DLK-1 as both a diagnostic marker and potential therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May have a role in neuroendocrine differentiation.
See full target information DLK1
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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:7698 PubMed39227582

2024

Efficient generation of human NOTCH ligand-expressing haemogenic endothelial cells as infrastructure for in vitro haematopoiesis and lymphopoiesis.

Applications

Unspecified application

Species

Unspecified reactive species

Shicheng Sun,Ali Motazedian,Jacky Y Li,Kevin Wijanarko,Joe Jiang Zhu,Kothila Tharmarajah,Kathleen A Strumila,Anton Shkaruta,L Rayburn Nigos,Jacqueline V Schiesser,Yi Yu,Paul J Neeson,Elizabeth S Ng,Andrew G Elefanty,Edouard G Stanley

Molecular biology reports 51:203 PubMed38270651

2024

Macrophages regulate healing-associated fibroblasts in diabetic wound.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Xiao,Jieqi Qian,Xiaohui Deng,Huifeng Zhang,Jiancheng Wang,Zhijun Luo,Lingyan Zhu

eLife 12: PubMed37603466

2023

The negative adipogenesis regulator is transcriptionally regulated by (TIS7) and translationally by its orthologue (SKMc15).

Applications

Unspecified application

Species

Unspecified reactive species

Ilja Vietor,Domagoj Cikes,Kati Piironen,Theodora Vasakou,David Heimdörfer,Ronald Gstir,Matthias David Erlacher,Ivan Tancevski,Philipp Eller,Egon Demetz,Michael W Hess,Volker Kuhn,Gerald Degenhart,Jan Rozman,Martin Klingenspor,Martin Hrabe de Angelis,Taras Valovka,Lukas A Huber

Nature metabolism 4:1591-1610 PubMed36400935

2022

Single-cell profiling of vascular endothelial cells reveals progressive organ-specific vulnerabilities during obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Olga Bondareva,Jesús Rafael Rodríguez-Aguilera,Fabiana Oliveira,Longsheng Liao,Alina Rose,Anubhuti Gupta,Kunal Singh,Florian Geier,Jenny Schuster,Jes-Niels Boeckel,Joerg M Buescher,Shrey Kohli,Nora Klöting,Berend Isermann,Matthias Blüher,Bilal N Sheikh

Nature communications 13:2464 PubMed35513363

2022

Epigenetic changes induced by in utero dietary challenge result in phenotypic variability in successive generations of mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mathew Van de Pette,Andrew Dimond,António M Galvão,Steven J Millership,Wilson To,Chiara Prodani,Gráinne McNamara,Ludovica Bruno,Alessandro Sardini,Zoe Webster,James McGinty,Paul M W French,Anthony G Uren,Juan Castillo-Fernandez,William Watkinson,Anne C Ferguson-Smith,Matthias Merkenschlager,Rosalind M John,Gavin Kelsey,Amanda G Fisher

Cell cycle (Georgetown, Tex.) 21:851-873 PubMed35156543

2022

LncRNA LINC01315 silencing modulates cancer stem cell properties and epithelial-to-mesenchymal transition in colorectal cancer via miR-484/DLK1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Youran Li,Wei Wang,Minna Wu,Ping Zhu,Zailong Zhou,Yuxia Gong,Yunfei Gu

Nature neuroscience 24:584-594 PubMed33723434

2021

Single-cell atlas of early human brain development highlights heterogeneity of human neuroepithelial cells and early radial glia.

Applications

Unspecified application

Species

Unspecified reactive species

Ugomma C Eze,Aparna Bhaduri,Maximilian Haeussler,Tomasz J Nowakowski,Arnold R Kriegstein

Frontiers in veterinary science 7:577835 PubMed33195583

2020

Anti-fibrotic Actions of Equine Interleukin-10 on Transforming Growth Factor-Beta1-Stimulated Dermal Fibroblasts Isolated From Limbs of Horses.

Applications

Unspecified application

Species

Unspecified reactive species

Lyn M Wise,Gabriella S Stuart,Kevalee Sriutaisuk,Brooke R Adams,Christopher B Riley,Christine L Theoret

Scientific reports 10:12346 PubMed32704071

2020

Prophylactic treatment with transdermal deferoxamine mitigates radiation-induced skin fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Abra H Shen,Mimi R Borrelli,Sandeep Adem,Nestor M Diaz Deleon,Ronak A Patel,Shamik Mascharak,Sara J Yen,Blake Y Sun,Walter L Taylor,Michael Januszyk,Dung H Nguyen,Arash Momeni,Geoffrey C Gurtner,Michael T Longaker,Derrick C Wan

Stem cells (Dayton, Ohio) 38:382-389 PubMed31793745

2019

Fat grafting rescues radiation-induced joint contracture.

Applications

Unspecified application

Species

Unspecified reactive species

Mimi R Borrelli,Nestor M Diaz Deleon,Sandeep Adem,Ronak A Patel,Shamik Mascharak,Abra H Shen,Dre Irizarry,Dung Nguyen,Arash Momeni,Michael T Longaker,Derrick C Wan
View all publications
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Product promise

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