Mouse Monoclonal DLK-1 antibody. Suitable for Flow Cyt, ELISA, WB, ICC/IF and reacts with Mouse, Human, Synthetic peptide, Recombinant full length protein samples. Cited in 12 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DLK1 aa 150-350.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-DLK-1 antibody [3A10] (AB119930), expandable thumbnail](https://content.abcam.com/products/images/dlk-1-antibody-3a10-ab119930--immunocytochemistry-immunofluorescence-img82821.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-DLK-1 antibody [3A10] (AB119930)")
![Flow Cytometry - Anti-DLK-1 antibody [3A10] (AB119930), expandable thumbnail](https://content.abcam.com/products/images/dlk-1-antibody-3a10-ab119930--flow-cytometry-img83973.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-DLK-1 antibody [3A10] (AB119930)")
![Western blot - Anti-DLK-1 antibody [3A10] (AB119930), expandable thumbnail](https://content.abcam.com/products/images/dlk-1-antibody-3a10-ab119930--western-blot-img136498.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DLK-1 antibody [3A10] (AB119930)")
![ELISA - Anti-DLK-1 antibody [3A10] (AB119930), expandable thumbnail](https://content.abcam.com/products/images/dlk-1-antibody-3a10-ab119930--elisa-img136543.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-DLK-1 antibody [3A10] (AB119930)")
Publications
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- Nature metabolism 4:1591-16102022Single-cell profiling of vascular endothelial cells reveals progressive organ-specific vulnerabilities during obesity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOlga Bondareva,Jesús Rafael Rodríguez-Aguilera,Fabiana Oliveira,Longsheng Liao,Alina Rose,Anubhuti Gupta,Kunal Singh,Florian Geier,Jenny Schuster,Jes-Niels Boeckel,Joerg M Buescher,Shrey Kohli,Nora Klöting,Berend Isermann,Matthias Blüher,Bilal N SheikhPubMed 36400935
- Cell cycle (Georgetown, Tex.) 21:851-8732022LncRNA LINC01315 silencing modulates cancer stem cell properties and epithelial-to-mesenchymal transition in colorectal cancer via miR-484/DLK1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYouran Li,Wei Wang,Minna Wu,Ping Zhu,Zailong Zhou,Yuxia Gong,Yunfei GuPubMed 35156543
- Nature neuroscience 24:584-5942021Single-cell atlas of early human brain development highlights heterogeneity of human neuroepithelial cells and early radial glia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesUgomma C Eze,Aparna Bhaduri,Maximilian Haeussler,Tomasz J Nowakowski,Arnold R KriegsteinPubMed 33723434
- Frontiers in veterinary science 7:5778352020Anti-fibrotic Actions of Equine Interleukin-10 on Transforming Growth Factor-Beta1-Stimulated Dermal Fibroblasts Isolated From Limbs of Horses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLyn M Wise et. al.PubMed 33195583
- Scientific reports 10:123462020Prophylactic treatment with transdermal deferoxamine mitigates radiation-induced skin fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbra H Shen et. al.PubMed 32704071
- Stem cells (Dayton, Ohio) 38:382-3892019Fat grafting rescues radiation-induced joint contracture.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMimi R Borrelli,Nestor M Diaz Deleon,Sandeep Adem,Ronak A Patel,Shamik Mascharak,Abra H Shen,Dre Irizarry,Dung Nguyen,Arash Momeni,Michael T Longaker,Derrick C WanPubMed 31793745
- Development (Cambridge, England) 146:2019CDK5RAP3, a UFL1 substrate adaptor, is crucial for liver development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Yang et. al.PubMed 30635284
- Gene 691:132-1402018Novel role of SF1 in alleviating thyroid-associated ophthalmopathy through the AMPK/mTOR signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Xia Wang et. al.PubMed 30562606
- Acta neuropathologica 135:779-7982018Paragangliomas arise through an autonomous vasculo-angio-neurogenic program inhibited by imatinib.
- Proceedings of the National Academy of Sciences of 114:E771-E7802017Atrial natriuretic peptide regulates adipose tissue accumulation in adult atria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNadine Suffee et. al.PubMed 28096344
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human DLK1 aa 150-350. The exact immunogen used to generate this antibody is proprietary information. Database link P80370
Abcam Recommends
Reactivity data
Select an application| Flow Cyt | ELISA | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Expected | Expected | Expected | Tested |
| Mouse | Tested | Expected | Expected | Expected |
| Recombinant full length protein | Not recommended | Not recommended | Tested | Not recommended |
| Synthetic peptide | Not recommended | Tested | Not recommended | Not recommended |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/200.00000 - 1/400.00000 | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide, Recombinant full length protein | Dilution info - | Notes - |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 1/10000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide, Recombinant full length protein | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
May have a role in neuroendocrine differentiation.
Alternative names
DLK, DLK1, Protein delta homolog 1, DLK-1, pG2
Recommended products
Mouse Monoclonal DLK-1 antibody. Suitable for Flow Cyt, ELISA, WB, ICC/IF and reacts with Mouse, Human, Synthetic peptide, Recombinant full length protein samples. Cited in 12 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DLK1 aa 150-350.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human DLK1 aa 150-350. The exact immunogen used to generate this antibody is proprietary information. Database link P80370
- Clone number
- 3A10
- Purification technique
- Affinity purification Protein G
- Light chain type
- kappa
- Concentration
- Purification notes
Purified from tissue culture supernatant.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Delta-like 1 homolog (DLK-1) also known as Pref-1 (preadipocyte factor 1) is a protein involved in a wide range of cellular processes. The mass of DLK-1 is approximately 65 kDa. This protein is expressed in various tissues including the liver brain and endocrine glands. DLK-1 has an important role in the regulation of cell differentiation and growth acting as a signal molecule that influences tissue development and regeneration.
- Biological function summary
DLK-1 serves a function in the inhibition of adipocyte differentiation and the maintenance of preadipocyte phenotype. It does not form part of a complex but interacts with membrane-bound receptors to mediate signaling pathways that impact tissue development. Researchers study its role in embryonic development and its downregulation is a significant factor during the differentiation of mesenchymal cells. Due to these interactions DLK-1 has a pivotal involvement in processes governing tissue regeneration and growth control.
- Pathways
Better understanding of DLK-1 involves examining its interaction in the Notch signaling pathway. This important pathway related to cell fate decisions features pathways with other proteins like Notch-1 and Notch-3. The relationship helps modulate the fate and proliferation of cells. DLK-1 also has associations with pathways involving phosphoinositide 3-kinase (PI3K) which influences cell growth and survival through related proteins such as AKT.
- Associated diseases and disorders
DLK-1 has shown associations with cancer and metabolic disorders. Overexpression of DLK-1 is linked to obesity due to its impact on preadipocyte differentiation. Additionally higher levels of DLK-1 can correlate with certain cancer progression where it acts alongside proteins like Notch-1 in promoting tumor cell growth. Understanding DLK-1's role in these contexts highlights the importance of DLK-1 as both a diagnostic marker and potential therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunocytochemistry/ Immunofluorescence - Anti-DLK-1 antibody [3A10] (ab119930), expandable thumbnail](https://content.abcam.com/products/images/dlk-1-antibody-3a10-ab119930--immunocytochemistry-immunofluorescence-img82821.jpg "Immunocytochemistry/ Immunofluorescence - Anti-DLK-1 antibody [3A10] (ab119930)")
Immunocytochemistry/ Immunofluorescence - Anti-DLK-1 antibody [3A10] (ab119930)
Immunofluorescence analysis of U251 cells using ab119930 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
![Flow Cytometry - Anti-DLK-1 antibody [3A10] (ab119930), expandable thumbnail](https://content.abcam.com/products/images/dlk-1-antibody-3a10-ab119930--flow-cytometry-img83973.jpg "Flow Cytometry - Anti-DLK-1 antibody [3A10] (ab119930)")
Flow Cytometry - Anti-DLK-1 antibody [3A10] (ab119930)
Flow cytometric analysis of NIH3T3 cells using ab119930 at 1/200 dilution (green) and negative control (red).
![Western blot - Anti-DLK-1 antibody [3A10] (ab119930), expandable thumbnail](https://content.abcam.com/products/images/dlk-1-antibody-3a10-ab119930--western-blot-img136498.jpg "Western blot - Anti-DLK-1 antibody [3A10] (ab119930)")
Western blot - Anti-DLK-1 antibody [3A10] (ab119930)
All lanes: Western blot - Anti-DLK-1 antibody [3A10] (ab119930) at 1/500 dilution
All lanes: Recombinant DLK-1 protein
Predicted band size: 21 kDa, 25 kDa, 31 kDa, 37 kDa, 39 kDa, 41 kDa, 68 kDa, 84 kDa
![ELISA - Anti-DLK-1 antibody [3A10] (ab119930), expandable thumbnail](https://content.abcam.com/products/images/dlk-1-antibody-3a10-ab119930--elisa-img136543.jpg "ELISA - Anti-DLK-1 antibody [3A10] (ab119930)")
ELISA - Anti-DLK-1 antibody [3A10] (ab119930)
ELISA using ab119930.
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