Rabbit Polyclonal DLK-1 antibody. Suitable for WB and reacts with Mouse samples. Cited in 32 publications.
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- Journal of the Endocrine Society 6:bvac1272022Precocious Puberty in a Boy With Bilateral Leydig Cell Tumors due to a Somatic Gain-of-Function Variant.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChelsi Flippo,Vipula Kolli,Melissa Andrew,Seth Berger,Tricia Bhatti,Alison M Boyce,Daniel Casella,Michael T Collins,Emmanuèle Délot,Joseph Devaney,Stephen M Hewitt,Thomas Kolon,Ashwini Mallappa,Perrin C White,Deborah P Merke,Andrew DauberPubMed 36111273
- Histochemistry and cell biology 158:169-1802022Aniridia-related keratopathy relevant cell signaling pathways in human fetal corneas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndré Vicente,Marta Sloniecka,Jing-Xia Liu,Berit Byström,Fátima Pedrosa DomellöfPubMed 35551459
- Frontiers in immunology 12:7163142021Splenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrest.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCaroline Vilas Boas de Melo,Felipe Guimarães Torres,Micely D'El-Rei Hermida,Jonathan L M Fontes,Bianca Ramos Mesquita,Reginaldo Brito,Pablo Ivan P Ramos,Gabriel R Fernandes,Luiz Antônio Rodrigues Freitas,Ricardo Khouri,Carlos Henrique Nery Costa,Washington L C Dos-SantosPubMed 34804009
- Nature neuroscience 24:584-5942021Single-cell atlas of early human brain development highlights heterogeneity of human neuroepithelial cells and early radial glia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesUgomma C Eze,Aparna Bhaduri,Maximilian Haeussler,Tomasz J Nowakowski,Arnold R KriegsteinPubMed 33723434
- Cancers 13:2021Comprehensive Assessment of Copy Number Alterations Uncovers Recurrent and Copy Gain in Medullary Thyroid Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAline Neves Araujo et. al.PubMed 33435319
- Nature communications 11:56532020Injury triggers fascia fibroblast collective cell migration to drive scar formation through N-cadherin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongsheng Jiang et. al.PubMed 33159076
- Communications biology 3:6422020Embryonic liver developmental trajectory revealed by single-cell RNA sequencing in the Foxa2 mouse.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTianhao Mu et. al.PubMed 33144666
- Cell biology international 45:295-3042020Soluble factors released by dedifferentiated fat cells reduce the functional activity of iPS cell-derived cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHirofumi Watanabe,Kazunori Kanemaru,Kazuhiro Hagikura,Taro Matsumoto,Mamoru Ayusawa,Ichiro MoriokaPubMed 33073424
- Cell stem cell 27:951-961.e52020SARS-CoV-2 Infects the Brain Choroid Plexus and Disrupts the Blood-CSF Barrier in Human Brain Organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura Pellegrini et. al.PubMed 33113348
- Oncogene 39:4028-40442020Hypoxia-induced release, nuclear translocation, and signaling activity of a DLK1 intracellular fragment in glioma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElisa Stellaria Grassi et. al.PubMed 32205867
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Predicted |
| Mouse | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000 | Notes Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Function
May have a role in neuroendocrine differentiation.
Alternative names
DLK, DLK1, Protein delta homolog 1, DLK-1, pG2
Recommended products
Rabbit Polyclonal DLK-1 antibody. Suitable for WB and reacts with Mouse samples. Cited in 32 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Delta-like 1 homolog (DLK-1) also known as Pref-1 (preadipocyte factor 1) is a protein involved in a wide range of cellular processes. The mass of DLK-1 is approximately 65 kDa. This protein is expressed in various tissues including the liver brain and endocrine glands. DLK-1 has an important role in the regulation of cell differentiation and growth acting as a signal molecule that influences tissue development and regeneration.
- Biological function summary
DLK-1 serves a function in the inhibition of adipocyte differentiation and the maintenance of preadipocyte phenotype. It does not form part of a complex but interacts with membrane-bound receptors to mediate signaling pathways that impact tissue development. Researchers study its role in embryonic development and its downregulation is a significant factor during the differentiation of mesenchymal cells. Due to these interactions DLK-1 has a pivotal involvement in processes governing tissue regeneration and growth control.
- Pathways
Better understanding of DLK-1 involves examining its interaction in the Notch signaling pathway. This important pathway related to cell fate decisions features pathways with other proteins like Notch-1 and Notch-3. The relationship helps modulate the fate and proliferation of cells. DLK-1 also has associations with pathways involving phosphoinositide 3-kinase (PI3K) which influences cell growth and survival through related proteins such as AKT.
- Associated diseases and disorders
DLK-1 has shown associations with cancer and metabolic disorders. Overexpression of DLK-1 is linked to obesity due to its impact on preadipocyte differentiation. Additionally higher levels of DLK-1 can correlate with certain cancer progression where it acts alongside proteins like Notch-1 in promoting tumor cell growth. Understanding DLK-1's role in these contexts highlights the importance of DLK-1 as both a diagnostic marker and potential therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
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1 product image
Western blot - Anti-DLK-1 antibody (ab21682)
This blot was produced using an 8% Tris-glycine gel under the Tris-glycine buffer system. The gel was run at 120V for 1 hour 15 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab21682 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
All lanes: Western blot - Anti-DLK-1 antibody (ab21682) at 1/2000 dilution
Lane 1: E18.5 Mouse Muscle Wild Type Tissue Lysate at 5 µg
Lane 2: E18.5 Mouse Muscle DLK1 Knockout Tissue Lysate at 5 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 15 kDa, 45 kDa, 48 kDa, 55 kDa
Exposure time: 3min
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