AB21682

Anti-DLK-1 antibody

Name: Anti-DLK-1 antibody (ab21682) | Abcam
Brand: Abcam Limited
SKU: AB21682
Price: 460 USD
Availability: InStock
Rating: 4 (5 reviews)

(5 Reviews)

(37 Publications)

Rabbit Polyclonal DLK-1 antibody. Suitable for WB and reacts with Mouse samples. Cited in 37 publications.

View Alternative Names

DLK, DLK1, Protein delta homolog 1, DLK-1, pG2

1 Images

Western blot - Anti-DLK-1 antibody (AB21682)

This blot was produced using an 8% Tris-glycine gel under the Tris-glycine buffer system. The gel was run at 120V for 1 hour 15 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab21682 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

All lanes:

Western blot - Anti-DLK-1 antibody (ab21682) at 1/2000 dilution

Lane 1:

E18.5 Mouse Muscle Wild Type Tissue Lysate at 5 µg

Lane 2:

E18.5 Mouse Muscle DLK1 Knockout Tissue Lysate at 5 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

Predicted band size: 41 kDa

Observed band size: 15 kDa,45 kDa,48 kDa,55 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p>Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Delta-like 1 homolog (DLK-1) also known as Pref-1 (preadipocyte factor 1) is a protein involved in a wide range of cellular processes. The mass of DLK-1 is approximately 65 kDa. This protein is expressed in various tissues including the liver brain and endocrine glands. DLK-1 has an important role in the regulation of cell differentiation and growth acting as a signal molecule that influences tissue development and regeneration.

Biological function summary

DLK-1 serves a function in the inhibition of adipocyte differentiation and the maintenance of preadipocyte phenotype. It does not form part of a complex but interacts with membrane-bound receptors to mediate signaling pathways that impact tissue development. Researchers study its role in embryonic development and its downregulation is a significant factor during the differentiation of mesenchymal cells. Due to these interactions DLK-1 has a pivotal involvement in processes governing tissue regeneration and growth control.

Pathways

Better understanding of DLK-1 involves examining its interaction in the Notch signaling pathway. This important pathway related to cell fate decisions features pathways with other proteins like Notch-1 and Notch-3. The relationship helps modulate the fate and proliferation of cells. DLK-1 also has associations with pathways involving phosphoinositide 3-kinase (PI3K) which influences cell growth and survival through related proteins such as AKT.

DLK-1 has shown associations with cancer and metabolic disorders. Overexpression of DLK-1 is linked to obesity due to its impact on preadipocyte differentiation. Additionally higher levels of DLK-1 can correlate with certain cancer progression where it acts alongside proteins like Notch-1 in promoting tumor cell growth. Understanding DLK-1's role in these contexts highlights the importance of DLK-1 as both a diagnostic marker and potential therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May have a role in neuroendocrine differentiation.

See full target information DLK1

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Publications (37)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:5511 PubMed40595495

2025

Identification of the Notch ligand DLK1 as an immunotherapeutic target and regulator of tumor cell plasticity and chemoresistance in adrenocortical carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Nai-Yun Sun,Suresh Kumar,Yoo Sun Kim,Diana Varghese,Arnulfo Mendoza,Rosa Nguyen,Reona Okada,Karlyne Reilly,Brigitte Widemann,Yves Pommier,Fathi Elloumi,Anjali Dhall,Daiki Taniyama,Mayank Patel,Etan Aber,Cristina F Contreras,Rosandra N Kaplan,Katja Kiseljak-Vassiliades,Margaret E Wierman,Dan Martinez,Jennifer Pogoriler,Amber K Hamilton,Sharon J Diskin,John M Maris,Robert W Robey,Michael M Gottesman,Jaydira Del Rivero,Nitin Roper

Advanced healthcare materials 14:e2402606 PubMed39955738

2025

3D Bioprinted Coaxial Testis Model Using Human Induced Pluripotent Stem Cells:A Step Toward Bicompartmental Cytoarchitecture and Functionalization.

Applications

Unspecified application

Species

Unspecified reactive species

Meghan A Robinson,Sonia Hy Kung,Khaled Ym Youssef,Kali M Scheck,Robert H Bell,Funda Sar,Anne M Haegert,M Mahdi Asmae,Changfeng Cheng,Salina V Yeack,Bhairvi T Mathur,Feng Jiang,Colin C Collins,Faraz Hach,Stephanie M Willerth,Ryan K Flannigan

Journal of the Endocrine Society 6:bvac127 PubMed36111273

2022

Precocious Puberty in a Boy With Bilateral Leydig Cell Tumors due to a Somatic Gain-of-Function Variant.

Applications

Unspecified application

Species

Unspecified reactive species

Chelsi Flippo,Vipula Kolli,Melissa Andrew,Seth Berger,Tricia Bhatti,Alison M Boyce,Daniel Casella,Michael T Collins,Emmanuèle Délot,Joseph Devaney,Stephen M Hewitt,Thomas Kolon,Ashwini Mallappa,Perrin C White,Deborah P Merke,Andrew Dauber

Cellular and molecular life sciences : CMLS 79:386 PubMed35760917

2022

Maternal gut microbiota Bifidobacterium promotes placental morphogenesis, nutrient transport and fetal growth in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jorge Lopez-Tello,Zoe Schofield,Raymond Kiu,Matthew J Dalby,Douwe van Sinderen,Gwénaëlle Le Gall,Amanda N Sferruzzi-Perri,Lindsay J Hall

Histochemistry and cell biology 158:169-180 PubMed35551459

2022

Aniridia-related keratopathy relevant cell signaling pathways in human fetal corneas.

Applications

Unspecified application

Species

Unspecified reactive species

André Vicente,Marta Sloniecka,Jing-Xia Liu,Berit Byström,Fátima Pedrosa Domellöf

Frontiers in immunology 12:716314 PubMed34804009

2021

Splenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrest.

Applications

Unspecified application

Species

Unspecified reactive species

Caroline Vilas Boas de Melo,Felipe Guimarães Torres,Micely D'El-Rei Hermida,Jonathan L M Fontes,Bianca Ramos Mesquita,Reginaldo Brito,Pablo Ivan P Ramos,Gabriel R Fernandes,Luiz Antônio Rodrigues Freitas,Ricardo Khouri,Carlos Henrique Nery Costa,Washington L C Dos-Santos

Nature neuroscience 24:584-594 PubMed33723434

2021

Single-cell atlas of early human brain development highlights heterogeneity of human neuroepithelial cells and early radial glia.

Applications

Unspecified application

Species

Unspecified reactive species

Ugomma C Eze,Aparna Bhaduri,Maximilian Haeussler,Tomasz J Nowakowski,Arnold R Kriegstein

Cancers 13: PubMed33435319

2021

Comprehensive Assessment of Copy Number Alterations Uncovers Recurrent and Copy Gain in Medullary Thyroid Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Aline Neves Araujo,Cléber Pinto Camacho,Thais Biude Mendes,Susan Chow Lindsey,Lais Moraes,Marta Miyazawa,Rosana Delcelo,Renata Pellegrino,Diego Robles Mazzotti,Rui Monteiro de Barros Maciel,Janete Maria Cerutti

Nature communications 11:5653 PubMed33159076

2020

Injury triggers fascia fibroblast collective cell migration to drive scar formation through N-cadherin.

Applications

Unspecified application

Species

Unspecified reactive species

Dongsheng Jiang,Simon Christ,Donovan Correa-Gallegos,Pushkar Ramesh,Shruthi Kalgudde Gopal,Juliane Wannemacher,Christoph H Mayr,Valerio Lupperger,Qing Yu,Haifeng Ye,Martin Mück-Häusl,Vijayanand Rajendran,Li Wan,Juan Liu,Ursula Mirastschijski,Thomas Volz,Carsten Marr,Herbert B Schiller,Yuval Rinkevich

Communications biology 3:642 PubMed33144666

2020

Embryonic liver developmental trajectory revealed by single-cell RNA sequencing in the Foxa2 mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Tianhao Mu,Liqin Xu,Yu Zhong,Xinyu Liu,Zhikun Zhao,Chaoben Huang,Xiaofeng Lan,Chengchen Lufei,Yi Zhou,Yixun Su,Luang Xu,Miaomiao Jiang,Hongpo Zhou,Xinxin Lin,Liang Wu,Siqi Peng,Shiping Liu,Susanne Brix,Michael Dean,Norris R Dunn,Kenneth S Zaret,Xin-Yuan Fu,Yong Hou

View all publications

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