Rabbit Recombinant Monoclonal DLK-1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat samples.
Images
![Western blot - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (AB230380), expandable thumbnail](https://content.abcam.com/products/images/dlk-1-antibody-epr19830-bsa-and-azide-free-ab230380--western-blot-img99112.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (AB230380)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (AB230380), expandable thumbnail](https://content.abcam.com/products/images/dlk-1-antibody-epr19830-bsa-and-azide-free-ab230380--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103493.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (AB230380)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (AB230380), expandable thumbnail](https://content.abcam.com/products/images/dlk-1-antibody-epr19830-bsa-and-azide-free-ab230380--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102644.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (AB230380)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (AB230380), expandable thumbnail](https://content.abcam.com/products/images/dlk-1-antibody-epr19830-bsa-and-azide-free-ab230380--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img34231.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (AB230380)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Mouse | Tested | Expected |
| Rat | Expected | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
May have a role in neuroendocrine differentiation. Inhibits adipocyte differentiation.
Alternative names
Dlk, Pref1, Scp-1, Dlk1, Protein delta homolog 1, DLK-1, Adipocyte differentiation inhibitor protein, Preadipocyte factor 1, Pref-1
Recommended products
Rabbit Recombinant Monoclonal DLK-1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR19830
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab230380 is the carrier-free version of Anti-DLK-1 antibody [EPR19830] ab210471.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Delta-like 1 homolog (DLK-1) also known as Pref-1 (preadipocyte factor 1) is a protein involved in a wide range of cellular processes. The mass of DLK-1 is approximately 65 kDa. This protein is expressed in various tissues including the liver brain and endocrine glands. DLK-1 has an important role in the regulation of cell differentiation and growth acting as a signal molecule that influences tissue development and regeneration.
- Biological function summary
DLK-1 serves a function in the inhibition of adipocyte differentiation and the maintenance of preadipocyte phenotype. It does not form part of a complex but interacts with membrane-bound receptors to mediate signaling pathways that impact tissue development. Researchers study its role in embryonic development and its downregulation is a significant factor during the differentiation of mesenchymal cells. Due to these interactions DLK-1 has a pivotal involvement in processes governing tissue regeneration and growth control.
- Pathways
Better understanding of DLK-1 involves examining its interaction in the Notch signaling pathway. This important pathway related to cell fate decisions features pathways with other proteins like Notch-1 and Notch-3. The relationship helps modulate the fate and proliferation of cells. DLK-1 also has associations with pathways involving phosphoinositide 3-kinase (PI3K) which influences cell growth and survival through related proteins such as AKT.
- Associated diseases and disorders
DLK-1 has shown associations with cancer and metabolic disorders. Overexpression of DLK-1 is linked to obesity due to its impact on preadipocyte differentiation. Additionally higher levels of DLK-1 can correlate with certain cancer progression where it acts alongside proteins like Notch-1 in promoting tumor cell growth. Understanding DLK-1's role in these contexts highlights the importance of DLK-1 as both a diagnostic marker and potential therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380), expandable thumbnail](https://content.abcam.com/products/images/dlk-1-antibody-epr19830-bsa-and-azide-free-ab230380--western-blot-img99112.jpg "Western blot - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)")
Western blot - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)
Blocking/Dilution buffer: 5% NFDM/TBST.
The expression pattern is consistent with the literature (PMID: 21419176 and PMID: 19247431).
The tissue lysates were kindly provided by our collaborator Prof. Anne Ferguson-Smith, Cambridge University.
The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DLK-1 antibody [EPR19830] ab210471).
All lanes: Western blot - Anti-DLK-1 antibody [EPR19830] (Anti-DLK-1 antibody [EPR19830] ab210471) at 1/1000 dilution
Lane 1: Mouse placenta lysate at 20 µg
Lane 2: DLK-1-knockout mouse placenta lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 50 kDa
Exposure time: 32s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380), expandable thumbnail](https://content.abcam.com/products/images/dlk-1-antibody-epr19830-bsa-and-azide-free-ab230380--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103493.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling DLK-1 with Anti-DLK-1 antibody [EPR19830] ab210471 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in the substantia nigra and ventral tegmental area (arrow) of mouse cerebrum (PMID: 19515692). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DLK-1 antibody [EPR19830] ab210471).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380), expandable thumbnail](https://content.abcam.com/products/images/dlk-1-antibody-epr19830-bsa-and-azide-free-ab230380--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102644.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)
Immunohistochemical analysis of paraffin-embedded mouse E14.5 embryo tissue labeling DLK-1 with Anti-DLK-1 antibody [EPR19830] ab210471 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in mouse E14.5 embryo (PMID: 16456855) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DLK-1 antibody [EPR19830] ab210471).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380), expandable thumbnail](https://content.abcam.com/products/images/dlk-1-antibody-epr19830-bsa-and-azide-free-ab230380--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img34231.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling DLK-1 with Anti-DLK-1 antibody [EPR19830] ab210471 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in the substantia nigra and ventral tegmental area (arrow) of rat cerebrum (PMID: 19515692) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DLK-1 antibody [EPR19830] ab210471).
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