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AB89908

Anti-DLK-1 antibody [MM0514-9D8]

5

(1 Review)

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(4 Publications)

Mouse Monoclonal DLK-1 antibody. Carrier free. Suitable for IHC-P, Flow Cyt, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DLK1.

View Alternative Names

DLK, DLK1, Protein delta homolog 1, DLK-1, pG2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-DLK-1 antibody [MM0514-9D8] (AB89908)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DLK-1 antibody [MM0514-9D8] (AB89908)

ICC/IF image of ab89908 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89908, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Flow Cytometry - Anti-DLK-1 antibody [MM0514-9D8] (AB89908)
  • Flow Cyt

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Flow Cytometry - Anti-DLK-1 antibody [MM0514-9D8] (AB89908)

Overlay histogram showing HepG2 cells stained with ab89908 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89908, 2μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min) used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [MM0514-9D8] (AB89908)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [MM0514-9D8] (AB89908)

IHC image of ab89908 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab89908, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MM0514-9D8

Isotype

IgG2b

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, ICC/IF, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human DLK1. The exact immunogen used to generate this antibody is proprietary information.

P80370

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p>We recommend to fix with 4% formaldehyde for 10min at room temperature.</p><p><a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and 0.2 µm filtered.
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Delta-like 1 homolog (DLK-1) also known as Pref-1 (preadipocyte factor 1) is a protein involved in a wide range of cellular processes. The mass of DLK-1 is approximately 65 kDa. This protein is expressed in various tissues including the liver brain and endocrine glands. DLK-1 has an important role in the regulation of cell differentiation and growth acting as a signal molecule that influences tissue development and regeneration.
Biological function summary

DLK-1 serves a function in the inhibition of adipocyte differentiation and the maintenance of preadipocyte phenotype. It does not form part of a complex but interacts with membrane-bound receptors to mediate signaling pathways that impact tissue development. Researchers study its role in embryonic development and its downregulation is a significant factor during the differentiation of mesenchymal cells. Due to these interactions DLK-1 has a pivotal involvement in processes governing tissue regeneration and growth control.

Pathways

Better understanding of DLK-1 involves examining its interaction in the Notch signaling pathway. This important pathway related to cell fate decisions features pathways with other proteins like Notch-1 and Notch-3. The relationship helps modulate the fate and proliferation of cells. DLK-1 also has associations with pathways involving phosphoinositide 3-kinase (PI3K) which influences cell growth and survival through related proteins such as AKT.

DLK-1 has shown associations with cancer and metabolic disorders. Overexpression of DLK-1 is linked to obesity due to its impact on preadipocyte differentiation. Additionally higher levels of DLK-1 can correlate with certain cancer progression where it acts alongside proteins like Notch-1 in promoting tumor cell growth. Understanding DLK-1's role in these contexts highlights the importance of DLK-1 as both a diagnostic marker and potential therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May have a role in neuroendocrine differentiation.
See full target information DLK1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of tissue engineering 12:20417314211019238 PubMed34104389

2021

Three dimensional porous scaffolds derived from collagen, elastin and fibrin proteins orchestrate adipose tissue regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Prasad Sawadkar,Nandin Mandakhbayar,Kapil D Patel,Jennifer Olmas Buitrago,Tae Hyun Kim,Poojitha Rajasekar,Ferdinand Lali,Christos Kyriakidis,Benyamin Rahmani,Jeviya Mohanakrishnan,Rishbha Dua,Karin Greco,Jung-Hwan Lee,Hae-Won Kim,Jonathan Knowles,Elena García-Gareta

eLife 9: PubMed32234209

2020

Functionally heterogeneous human satellite cells identified by single cell RNA sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Emilie Barruet,Steven M Garcia,Katharine Striedinger,Jake Wu,Solomon Lee,Lauren Byrnes,Alvin Wong,Sun Xuefeng,Stanley Tamaki,Andrew S Brack,Jason H Pomerantz

Cellular & molecular immunology 13:605-14 PubMed27593483

2016

Human CD34(lo)CD133(lo) fetal liver cells support the expansion of human CD34(hi)CD133(hi) hematopoietic stem cells.

Applications

ICC/IF

Species

Unspecified reactive species

Kylie Su Mei Yong,Choong Tat Keng,Shu Qi Tan,Eva Loh,Kenneth Te Chang,Thiam Chye Tan,Wanjin Hong,Qingfeng Chen

Nature communications 7:10243 PubMed26732624

2016

Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6.

Applications

IF

Species

Human

Patrick Guye,Mohammad R Ebrahimkhani,Nathan Kipniss,Jeremy J Velazquez,Eldi Schoenfeld,Samira Kiani,Linda G Griffith,Ron Weiss
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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