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AB325176

Anti-DLK (MAP3K12) antibody [EPR30420-541]

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Rabbit Recombinant Monoclonal MAP3K12 antibody. Suitable for I-ELISA, WB and reacts with Recombinant fragment - Human, Human, Mouse samples.

View Alternative Names

ZPK, MAP3K12, Mitogen-activated protein kinase kinase kinase 12, Dual leucine zipper bearing kinase, Leucine-zipper protein kinase, MAPK-upstream kinase, Mixed lineage kinase, DLK, MUK

3 Images
Western blot - Anti-DLK (MAP3K12) antibody [EPR30420-541] (AB325176)
  • WB

Lab

Western blot - Anti-DLK (MAP3K12) antibody [EPR30420-541] (AB325176)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : spleen, lung(PMID : 8769565; PMID : 7983011)

Low expression : HepG2, A431

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 35101451).

The identity of the bands below 75 kDa are unknown.

Lanes 1-3 are incubated with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 and lanes 4-7 are incubated with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

Exposure time : Lanes 1-3 : 70 seconds; Lanes 4-7 : 103 seconds

All lanes:

Western blot - Anti-DLK (MAP3K12) antibody [EPR30420-541] (ab325176) at 1/1000 dilution

Lane 1:

SK-N-BE(2) (human neuroblastoma neuroblast) whole lysate at 20 µg

Lane 2:

HepG2 (human hepatocellular carcinoma epithelial cell) whole lysate at 20 µg

Lane 3:

A431 (human epidermoid carcinoma epithelial cell) whole lysate at 20 µg

Lane 4:

Human hippocampus tissue lysate at 20 µg

Lane 5:

Human hypothalamus tissues lydate at 20 µg

Lane 6:

human spleen tissue lysate at 20 µg

Lane 7:

Human lung tissue lysate at 20 µg

Secondary

Lanes 1 - 3:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Lanes 4 - 7:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 120 kDa,36 kDa

false

Western blot - Anti-DLK (MAP3K12) antibody [EPR30420-541] (AB325176)
  • WB

Lab

Western blot - Anti-DLK (MAP3K12) antibody [EPR30420-541] (AB325176)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : spleen, heart, liver(PMID : 8769565; PMID : 7983011)

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 35101451).

The identity of the bands below 75 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-DLK (MAP3K12) antibody [EPR30420-541] (ab325176) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma neuroblast) at 20 µg

Lane 2:

Mouse hypothalamus tissue lysate at 20 µg

Lane 3:

Mouse cerebellum tissue lysate at 20 µg

Lane 4:

Mouse brain tissue lysate at 20 µg

Lane 5:

Mouse liver tissue lysate at 20 µg

Lane 6:

Mouse heart tissue lysate at 20 µg

Lane 7:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 120 kDa,36 kDa

false

Exposure time: 37s

Indirect ELISA - Anti-DLK (MAP3K12) antibody [EPR30420-541] (AB325176)
  • I-ELISA

Lab

Indirect ELISA - Anti-DLK (MAP3K12) antibody [EPR30420-541] (AB325176)

Indirect ELISA analysis of ab325176 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : DLK (MAP3K12);LZK (MAP3K13).

Antigen concentration : 1000 ng/ml

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR30420-541

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "31.25 ng/mL", "IELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of a non-canonical MAPK signaling pathway (PubMed : 28111074). Activated by APOE, enhances the AP-1-mediated transcription of APP, via a MAP kinase signal transduction pathway composed of MAP2K7 and MAPK1/ERK2 and MAPK3/ERK1 (PubMed : 28111074). May be an activator of the JNK/SAPK pathway.
See full target information MAP3K12
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com