AB229902

Anti-DLL3 antibody [EPR22592-18]

20ul selling size
BOND RX™ Validated
RabMAb
Recombinant
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(7 Publications)

Anti-DLL3 antibody [EPR22592-18] (ab229902) is a rabbit monoclonal antibody detecting DLL3 in Western Blot, Flow Cytometry (Intra), IP, IHC-P, ICC/IF. Suitable for Human, Mouse.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Delta-like protein 3, Drosophila Delta homolog 3, Delta3, DLL3

10 Images

Flow Cytometry (Intracellular) - Anti-DLL3 antibody [EPR22592-18] (AB229902)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-DLL3 antibody [EPR22592-18] (AB229902)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanolpermeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with DLL3 expression construct containing a myc-His-tag^ï¿½ labelling DLL3 with ab229902 at 1/600 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730, Left) isotype control.

Goat anti rabbit IgG (Alexa Fluor^ï¿½ 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Cells were stained with rabbit IgG (Left) or ab229902 (Right). Then stained with anti-myc-tag conjugated to Alexa Fluor^ï¿½ 647.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] (AB229902)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] (AB229902)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling DLL3 with ab229902 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : no staining on human cerebrum (PMID : 30397180) is observed.

The section was incubated with ab229902 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Immunocytochemistry/ Immunofluorescence - Anti-DLL3 antibody [EPR22592-18] (AB229902)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DLL3 antibody [EPR22592-18] (AB229902)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells labelling DLL3 with ab229902 at 1/100 dilution, followed by ab150080 AlexaFluor^®594 Goat anti-Rabbit secondary antibody at 1/500 dilution (Green). An anti-myc-tag mAb (Alexa Fluor^® 488 Conjugate) was used at 1/100 dilution. Confocal image showing positive staining in HEK-293T cells transfected with DLL3 expression construct containing a myc-His-tag^® is observed. The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150080 AlexaFluor^®594 Goat anti-Rabbit secondary at 1/500 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] (AB229902)

Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling DLL3 with ab229902 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human glioma (PMID : 30397180) is observed.

The section was incubated with ab229902 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] (AB229902)

Immunohistochemical analysis of paraffin-embedded human small cell lung cancer tissue labeling DLL3 with ab229902 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human small cell lung cancer (PMID : 28487384, 30397180) is observed.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab229902 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Unknown

Immunoprecipitation - Anti-DLL3 antibody [EPR22592-18] (AB229902)

DLL3 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney epithelial cell) transfected with DLL3 expression construct containing a myc-His-tag® whole cell lysate with ab229902 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab229902 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.

Lane 1 : HEK-293T (human embryonic kidney epithelial cell) transfected with DLL3 expression construct containing a myc-His-tag® whole cell lysate 10μg

Lane 2 : ab229902 IP in HEK-293T transfected with DLL3 expression construct containing a myc-His-tag^® whole cell lysate (Input).

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab229902 in HEK-293T transfected with DLL3 expression construct containing a myc-His-tag® whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 15 seconds.

All lanes:

Immunoprecipitation - Anti-DLL3 antibody [EPR22592-18] (ab229902)

Predicted band size: 65 kDa

Observed band size: 55-80 kDa

false

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] (AB229902)

Immunohistochemical analysis of paraffin-embedded mouse embryonic brain of day 14.5 tissue labeling DLL3 with ab229902 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse embryonic brain of day 14.5 (PMID : 19389376) is observed.

The section was incubated with ab229902 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] (AB229902)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling DLL3 with ab229902 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : no staining on mouse cerebrum (PMID : 30397180) is observed.

The section was incubated with ab229902 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Supplier Data

Western blot - Anti-DLL3 antibody [EPR22592-18] (AB229902)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-DLL3 antibody [EPR22592-18] (ab229902) at 1/1000 dilution

All lanes:

TT (human thyroid carcinoma epithelial cell) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 65 kDa

Observed band size: 65 kDa

false

Unknown

Western blot - Anti-DLL3 antibody [EPR22592-18] (AB229902)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-DLL3 antibody [EPR22592-18] (ab229902) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with DLL3 (WT) expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 2:

HEK-293T transfected with empty vector containing myc-His-tag®, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 65 kDa

Observed band size: 75 kDa

false

Carrier free

Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free
660 APC

APC Anti-DLL3 antibody [EPR22592-18]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-DLL3 antibody [EPR22592-18]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-DLL3 antibody [EPR22592-18]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-DLL3 antibody [EPR22592-18]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-DLL3 antibody [EPR22592-18]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-DLL3 antibody [EPR22592-18]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-DLL3 antibody [EPR22592-18]
HRP

HRP Anti-DLL3 antibody [EPR22592-18]
578 PE

PE Anti-DLL3 antibody [EPR22592-18]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22592-18

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, Flow Cyt (Intra), IHC-P, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The mouse recommendation is based on the IHC-P results. We do not guarantee WB for mouse.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/600", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

What is this antibody validated in?
Anti-DLL3 antibody [EPR22592-18] (ab229902) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.

What is the molecular weight of DLL3?
Anti-DLL3 [EPR22592-18] (ab229902) specifically detects a band for DLL3 (UniProt: Q9NYJ7) at a molecular weight of 65kDa.

Other related products
We have a range of other formats of antibody clone [EPR22592-18] also available for your convenience: ab229902, Carrier free - ab255694, PE - ab305808, APC - ab305809, HRP - ab305810, Alkaline Phosphatase - ab308729, Alexa Fluor^® 488 - ab309688, Alexa Fluor^® 647 - ab310054, Alexa Fluor^® 594 - ab310446, Alexa Fluor^® 555 - ab311973, Alexa Fluor^® 568 - ab312447, Alexa Fluor^® 750 - ab321469

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl. Discover our selection of trial-size antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DLL3 also known as Delta-like protein 3 is a member of the Delta/Serrate/Jagged family. This protein has a molecular mass of approximately 64 kDa. Scientists find DLL3 expression primarily in the Golgi apparatus of cells. In healthy tissues DLL3 is mostly present in the central nervous system whereas in abnormal conditions it appears in certain types of cancer cells. The detection of DLL3 expression commonly performed using IHC (immunohistochemistry) highlights its potential as a biomarker in pathological studies.

Biological function summary

DLL3 functions mainly in the modulation of the Notch signaling pathway. It does not activate this pathway but acts as an inhibitor blocking Notch ligand activation on adjacent cells. DLL3's role involves binding directly to Notch receptors altering cellular differentiation and growth processes. Despite being related to the Delta/Serrate/LAG-2 family DLL3 exhibits unique characteristics in its interactions forming part of a regulatory complex different from its family members.

Pathways

DLL3 engages importantly in the Notch signaling pathway affecting important cellular communications that regulate development and differentiation. Its interaction with proteins of the Notch family specifically the Notch receptors highlights its inhibitory role. Additionally DLL3 intersects with the Wnt signaling pathway though indirect influencing its output by modulating availability of pathway regulators.

DLL3 has significant implications in cancer notably small cell lung cancer (SCLC) and neuroendocrine tumors. Researchers associate increased DLL3 expression with aggressive tumor behavior and poor prognosis. The protein also shows relevance in congenital disorders such as spondylocostal dysostosis where abnormal DLL3 function leads to vertebral segmentation defects. In these contexts DLL3 often relates to proteins involved in the disruption of normal signaling cascades impacting both tumor progression and developmental anomalies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity).

See full target information DLL3

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 6:102217 PubMed40834855

2025

Genomic landscape and immunological profile of glioblastoma in East Asian patients.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng Zhong,Bo Wu,Frank Dubois,Davy Deng,Johann S Bergholz,Wanming Hu,Hao Duan,Yujie Shao,Jingyi Liu,Luwei Liu,Sitong Chen,Yongfei Hu,Zheng Zhao,Maojin Yao,Ke Sai,Jian Wang,Yinsheng Chen,Chao Ke,Xiangheng Zhang,Qunying Yang,Chengcheng Guo,Ji Zhang,Fuhua Lin,Zhenghe Chen,Deipei Li,Zhenqiang He,Chang Li,Xiaobing Jiang,Jie Lu,Wenzhuo Yang,Tao Jiang,Zhongping Chen,Rameen Beroukhim,Jean J Zhao,Yonggao Mou

Experimental hematology & oncology 14:35 PubMed40075480

2025

Engineered circular RNA-based DLL3-targeted CAR-T therapy for small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jingsheng Cai,Zheng Liu,Shaoyi Chen,Jingwei Zhang,Haoran Li,Xun Wang,Feng Yang,Shaodong Wang,Xiao Li,Yun Li,Kezhong Chen,Jun Wang,Ming Sun,Mantang Qiu

Clinical & experimental metastasis 42:12 PubMed39856383

2025

Repurposing neuroleptics: clozapine as a novel, adjuvant therapy for melanoma brain metastases.

Applications

Unspecified application

Species

Unspecified reactive species

Tobias Wikerholmen,Erlend Moen Taule,Emma Rigg,Birgitte Feginn Berle,Magnus Sættem,Katharina Sarnow,Halala Sdik Saed,Terje Sundstrøm,Frits Thorsen

Oncotarget 15:750-763 PubMed39392394

2024

Relationship between the expressions of DLL3, ASC1, TTF-1 and Ki-67: First steps of precision medicine at SCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel Silva,Juliana C Sousa,Cleto Nogueira,Raquel Feijo,Francisco Martins Neto,Laura Cardoso Marinho,Guilherme Sousa,Valeria Denninghoff,Fabio Tavora

Science (New York, N.Y.) 383:eadj1415 PubMed38330136

2024

Lineage-specific intolerance to oncogenic drivers restricts histological transformation.

Applications

Unspecified application

Species

Unspecified reactive species

Eric E Gardner,Ethan M Earlie,Kate Li,Jerin Thomas,Melissa J Hubisz,Benjamin D Stein,Chen Zhang,Lewis C Cantley,Ashley M Laughney,Harold Varmus

Biomedicines 11: PubMed37893184

2023

DLL3 Is a Prognostic and Potentially Predictive Biomarker for Immunotherapy Linked to PD/PD-L Axis and NOTCH1 in Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Carlos Lacalle-Gonzalez,Maria Florez-Cespedes,Lara Sanz-Criado,Michael Ochieng' Otieno,Edurne Ramos-Muñoz,Maria Jesus Fernandez-Aceñero,Luis Ortega-Medina,Jesus Garcia-Foncillas,Javier Martinez-Useros

Frontiers in genetics 14:1098190 PubMed37274780

2023

Identification of DLL3-related genes affecting the prognosis of patients with colon adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jinyu Xiang,Wenjing Gong,Jiannan Liu,Huijuan Zhang,Ming Li,Rujian Wang,Yaodong Lv,Ping Sun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DLL3 antibody [EPR22592-18]

Flow Cytometry (Intracellular) - Anti-DLL3 antibody [EPR22592-18] (AB229902)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] (AB229902)

Immunocytochemistry/ Immunofluorescence - Anti-DLL3 antibody [EPR22592-18] (AB229902)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] (AB229902)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] (AB229902)

Immunoprecipitation - Anti-DLL3 antibody [EPR22592-18] (AB229902)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] (AB229902)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] (AB229902)

Western blot - Anti-DLL3 antibody [EPR22592-18] (AB229902)

Western blot - Anti-DLL3 antibody [EPR22592-18] (AB229902)

Related conjugates and formulations (10)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Proteins & Peptides

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise