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Rabbit Recombinant Monoclonal DLL3 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (AB255694), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (AB255694), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (AB255694), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (AB255694), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (AB255694), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Not recommended
Expected
Expected
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

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8 products for Alternative Version

Target data

Function

Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity).

Alternative names

Delta-like protein 3, Drosophila Delta homolog 3, Delta3, DLL3

Recommended products

Rabbit Recombinant Monoclonal DLL3 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR22592-18
Purification technique
Affinity purification Protein A
Specificity

The mouse recommendation is based on the IHC-P results. We do not guarantee WB for mouse.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab255694 is the carrier-free version of Anti-DLL3 antibody [EPR22592-18] ab229902.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

DLL3 also known as Delta-like protein 3 is a member of the Delta/Serrate/Jagged family. This protein has a molecular mass of approximately 64 kDa. Scientists find DLL3 expression primarily in the Golgi apparatus of cells. In healthy tissues DLL3 is mostly present in the central nervous system whereas in abnormal conditions it appears in certain types of cancer cells. The detection of DLL3 expression commonly performed using IHC (immunohistochemistry) highlights its potential as a biomarker in pathological studies.

Biological function summary

DLL3 functions mainly in the modulation of the Notch signaling pathway. It does not activate this pathway but acts as an inhibitor blocking Notch ligand activation on adjacent cells. DLL3's role involves binding directly to Notch receptors altering cellular differentiation and growth processes. Despite being related to the Delta/Serrate/LAG-2 family DLL3 exhibits unique characteristics in its interactions forming part of a regulatory complex different from its family members.

Pathways

DLL3 engages importantly in the Notch signaling pathway affecting important cellular communications that regulate development and differentiation. Its interaction with proteins of the Notch family specifically the Notch receptors highlights its inhibitory role. Additionally DLL3 intersects with the Wnt signaling pathway though indirect influencing its output by modulating availability of pathway regulators.

Associated diseases and disorders

DLL3 has significant implications in cancer notably small cell lung cancer (SCLC) and neuroendocrine tumors. Researchers associate increased DLL3 expression with aggressive tumor behavior and poor prognosis. The protein also shows relevance in congenital disorders such as spondylocostal dysostosis where abnormal DLL3 function leads to vertebral segmentation defects. In these contexts DLL3 often relates to proteins involved in the disruption of normal signaling cascades impacting both tumor progression and developmental anomalies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694)

    Immunohistochemical analysis of paraffin-embedded human small cell lung cancer tissue labeling DLL3 with Anti-DLL3 antibody [EPR22592-18] ab229902 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human small cell lung cancer (PMID: 28487384, 30397180) is observed.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with Anti-DLL3 antibody [EPR22592-18] ab229902 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DLL3 antibody [EPR22592-18] ab229902).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694)

    Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling DLL3 with Anti-DLL3 antibody [EPR22592-18] ab229902 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human glioma (PMID: 30397180) is observed.

    The section was incubated with Anti-DLL3 antibody [EPR22592-18] ab229902 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DLL3 antibody [EPR22592-18] ab229902).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694)

    Immunohistochemical analysis of paraffin-embedded mouse embryonic brain of day 14.5 tissue labeling DLL3 with Anti-DLL3 antibody [EPR22592-18] ab229902 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse embryonic brain of day 14.5 (PMID: 19389376) is observed.

    The section was incubated with Anti-DLL3 antibody [EPR22592-18] ab229902 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DLL3 antibody [EPR22592-18] ab229902).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling DLL3 with Anti-DLL3 antibody [EPR22592-18] ab229902 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: no staining on human cerebrum (PMID: 30397180) is observed.

    The section was incubated with Anti-DLL3 antibody [EPR22592-18] ab229902 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DLL3 antibody [EPR22592-18] ab229902).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling DLL3 with Anti-DLL3 antibody [EPR22592-18] ab229902 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: no staining on mouse cerebrum (PMID: 30397180) is observed.

    The section was incubated with Anti-DLL3 antibody [EPR22592-18] ab229902 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DLL3 antibody [EPR22592-18] ab229902).

  • Immunoprecipitation - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694), expandable thumbnail

    Immunoprecipitation - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694)

    DLL3 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney epithelial cell) transfected with DLL3 expression construct containing a myc-His-tag® whole cell lysate with Anti-DLL3 antibody [EPR22592-18] ab229902 at 1/30 dilution. Western blot was performed on the immunoprecipitate using Anti-DLL3 antibody [EPR22592-18] ab229902 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used as the secondary antibody at 1/5000 dilution.

    Lane 1: HEK-293T (human embryonic kidney epithelial cell) transfected with DLL3 expression construct containing a myc-His-tag® whole cell lysate 10μg

    Lane 2: Anti-DLL3 antibody [EPR22592-18] ab229902 IP in HEK-293T transfected with DLL3 expression construct containing a myc-His-tag® whole cell lysate (Input).

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-DLL3 antibody [EPR22592-18] ab229902 in HEK-293T transfected with DLL3 expression construct containing a myc-His-tag® whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DLL3 antibody [EPR22592-18] ab229902).

    All lanes: Immunoprecipitation - Anti-DLL3 antibody [EPR22592-18] (Anti-DLL3 antibody [EPR22592-18] ab229902)

    Predicted band size: 65 kDa

    Observed band size: 55-80 kDa

  • Flow Cytometry (Intracellular) - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanolpermeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with DLL3 expression construct containing a myc-His-tag® labelling DLL3 with Anti-DLL3 antibody [EPR22592-18] ab229902 at 1/600 dilution (Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730, Left) isotype control.

    Goat anti rabbit IgG (Alexa Fluor®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG (Left) or Anti-DLL3 antibody [EPR22592-18] ab229902 (Right). Then stained with anti-myc-tag conjugated to Alexa Fluor®647.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DLL3 antibody [EPR22592-18] ab229902).

  • Immunocytochemistry/ Immunofluorescence - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells labelling DLL3 with Anti-DLL3 antibody [EPR22592-18] ab229902 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody at 1/500 dilution (Green). An anti-myc-tag mAb (Alexa Fluor® 488 Conjugate) was used at 1/100 dilution. Confocal image showing positive staining in HEK-293T cells transfected with DLL3 expression construct containing a myc-His-tag® is observed. The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DLL3 antibody [EPR22592-18] ab229902).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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