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AB176876

Anti-DLL4 antibody

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(8 Publications)

Rabbit Polyclonal DLL4 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DLL4 aa 150-350.

View Alternative Names

UNQ1895/PRO4341, DLL4, Delta-like protein 4, Drosophila Delta homolog 4, Delta4

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL4 antibody (AB176876)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL4 antibody (AB176876)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human fetal kidney tissue labeling DLL4 with ab176876 at 1/100 dilution.

Western blot - Anti-DLL4 antibody (AB176876)
  • WB

Supplier Data

Western blot - Anti-DLL4 antibody (AB176876)

All lanes:

Western blot - Anti-DLL4 antibody (ab176876) at 1/500 dilution

Lane 1:

Human fetal kidney lysate

Lane 2:

Human fetal lung lysate

Predicted band size: 74 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human DLL4 aa 150-350. The exact immunogen used to generate this antibody is proprietary information.

Q9NR61

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 200µL
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DLL4 also known as Delta-like ligand 4 is a member of the Delta/Serrate/Lag-2 family. This target is a single-pass type I membrane protein with a molecular weight of approximately 65 kDa. It features prominently in the vascular endothelium where it impacts angiogenic activity. DLL4 acts as a ligand for Notch receptors initiating a signaling cascade important to cell fate decisions. DLL4 expression occurs mainly on the arterial endothelium and the tumor vasculature highlighting its significance in angiogenesis.
Biological function summary

DLL4 engages in precise regulation of angiogenesis and vascular development. It participates in the Notch signaling pathway playing an integral role in controlling endothelial cell proliferation differentiation and migration. DLL4 does not operate in isolation; instead it functions as part of the Notch receptor-ligand complex. This relationship effectively regulates the sprouting of new blood vessels ensuring proper vascular patterning and functional blood supply.

Pathways

DLL4 links directly to the Notch signaling and VEGF (vascular endothelial growth factor) pathways both of which play critical roles in vascular development and homeostasis. Through the Notch pathway DLL4 interacts with Notch1 and Notch4 receptors facilitating communication that inhibits endothelial cell proliferation distinguishing it from other pro-angiogenic factors. Its relationship with the VEGF pathway allows DLL4 to modulate angiogenic processes balancing vessel sprouting by opposing excessive VEGF-induced activities.

Aberrations of DLL4 influence cancer and cardiovascular diseases. Overexpression and dysregulation of DLL4 have been observed in various cancers where it contributes to tumor angiogenesis promoting the growth and spread of cancer cells. In cardiovascular disorders dysfunctional DLL4 signaling impacts vascular development which can lead to fatal arteriovenous malformations. In these conditions DLL4 interacts with proteins like Notch1 and VEGF underlining its critical involvement in pathological angiogenesis and vessel formation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the Notch signaling pathway as Notch ligand (PubMed : 11134954). Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting (PubMed : 20616313). Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (By similarity). During spinal cord neurogenesis, inhibits V2a interneuron fate (PubMed : 17728344).
See full target information DLL4
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38791441

2024

BMPR2 Loss Activates AKT by Disrupting DLL4/NOTCH1 and PPARγ Signaling in Pulmonary Arterial Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Keytam S Awad,Shuibang Wang,Edward J Dougherty,Ali Keshavarz,Cumhur Y Demirkale,Zu Xi Yu,Latonia Miller,Jason M Elinoff,Robert L Danner

Biomedicines 11: PubMed36979893

2023

Knockdown Suppressed the Angiogenic Potential of Mesenchymal Stem Cells by Impacting CXCR4-Regulated MRTF-A SUMOylation and CCN1 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Zhang,Qingxi Liu,Cuicui Lyu,Xing Gao,Wenjian Ma

Canadian journal of gastroenterology & hepatology 2022:9202531 PubMed39296516

2022

Pumilio RNA-Binding Family Member 1 Plays a Promoting Role on Pancreatic Cancer Angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Haisu Dai,Yan Jiang,Zhipeng Liu,Xingxing Su,Yishi Yang,Zhiyu Chen

In vivo (Athens, Greece) 35:2771-2777 PubMed34410967

2021

Clinical Significance of TAP1 and DLL4 Expression in Patients With Locally Advanced Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kenki Segami,Toru Aoyama,Yukihiko Hiroshima,Keisuke Komori,Itaru Hashimoto,Hayato Watanabe,Kazuki Kano,Shinsuke Nagasawa,Masato Nakazono,Yukio Maezawa,Hirohito Fujikawa,Masakatsu Numata,Takanobu Yamada,Hiroshi Tamagawa,Naoto Yamamoto,Takashi Ogata,Manabu Siozawa,Norio Yukawa,Soichiro Morinaga,Yasushi Rino,Munetaka Masuda,Yohei Miyagi,Hiroshi Saeki,Takashi Oshima

Journal of Cancer 12:5914-5922 PubMed34476005

2021

Suppression of human colon tumor by EERAC through regulating Notch/DLL4/Hes pathway inhibiting angiogenesis .

Applications

Unspecified application

Species

Unspecified reactive species

Chenchen Yuan,Chenchen Wu,Rong Xue,Chun Jin,Chenguo Zheng

Acta dermato-venereologica 100:adv00059 PubMed31930426

2020

Increased Expression of Delta-like Ligand 4 in Mycosis Fungoides.

Applications

Unspecified application

Species

Unspecified reactive species

Hikari Boki,Tomomitsu Miyagaki,Yuki Shono,Hiroaki Kamijo,Tomonori Oka,Hiraku Suga,Yoshihide Asano,Makoto Sugaya,Shinichi Sato

Oncotarget 7:62976-62988 PubMed27542210

2016

The effect of epigenetic silencing and TP53 mutation on the expression of DLL4 in human cancer stem disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixing Yao,Zaki A Sherif

Endocrine-related cancer 23:665-76 PubMed27402614

2016

RET mutation and increased angiogenesis in medullary thyroid carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Antonella Verrienti,Giovanni Tallini,Chiara Colato,Amélie Boichard,Saula Checquolo,Valeria Pecce,Marialuisa Sponziello,Francesca Rosignolo,Dario de Biase,Kerry Rhoden,Gian Piero Casadei,Diego Russo,Michela Visani,Giorgia Acquaviva,Marco Ferdeghini,Sebastiano Filetti,Cosimo Durante
View all publications
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Product promise

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