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AB183532

Anti-DLL4 antibody

3

(1 Review)

|

(6 Publications)

Goat Polyclonal DLL4 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human DLL4 aa 550-600.

View Alternative Names

UNQ1895/PRO4341, DLL4, Delta-like protein 4, Drosophila Delta homolog 4, Delta4

1 Images
Western blot - Anti-DLL4 antibody (AB183532)
  • WB

Supplier Data

Western blot - Anti-DLL4 antibody (AB183532)

All lanes:

Western blot - Anti-DLL4 antibody (ab183532) at 0.3 µg/mL

Lane 1:

Human lung lysate at 35 µg

Lane 2:

Mouse lung lysate at 35 µg

Lane 3:

Rat lung lysate at 35 µg

Predicted band size: 74 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human DLL4 aa 550-600. The exact immunogen used to generate this antibody is proprietary information.

Q9NR61

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-1 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product.</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-1 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product.</p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-1 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab183532 was purified from goat serum by ammonium sulfate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DLL4 also known as Delta-like ligand 4 is a member of the Delta/Serrate/Lag-2 family. This target is a single-pass type I membrane protein with a molecular weight of approximately 65 kDa. It features prominently in the vascular endothelium where it impacts angiogenic activity. DLL4 acts as a ligand for Notch receptors initiating a signaling cascade important to cell fate decisions. DLL4 expression occurs mainly on the arterial endothelium and the tumor vasculature highlighting its significance in angiogenesis.
Biological function summary

DLL4 engages in precise regulation of angiogenesis and vascular development. It participates in the Notch signaling pathway playing an integral role in controlling endothelial cell proliferation differentiation and migration. DLL4 does not operate in isolation; instead it functions as part of the Notch receptor-ligand complex. This relationship effectively regulates the sprouting of new blood vessels ensuring proper vascular patterning and functional blood supply.

Pathways

DLL4 links directly to the Notch signaling and VEGF (vascular endothelial growth factor) pathways both of which play critical roles in vascular development and homeostasis. Through the Notch pathway DLL4 interacts with Notch1 and Notch4 receptors facilitating communication that inhibits endothelial cell proliferation distinguishing it from other pro-angiogenic factors. Its relationship with the VEGF pathway allows DLL4 to modulate angiogenic processes balancing vessel sprouting by opposing excessive VEGF-induced activities.

Aberrations of DLL4 influence cancer and cardiovascular diseases. Overexpression and dysregulation of DLL4 have been observed in various cancers where it contributes to tumor angiogenesis promoting the growth and spread of cancer cells. In cardiovascular disorders dysfunctional DLL4 signaling impacts vascular development which can lead to fatal arteriovenous malformations. In these conditions DLL4 interacts with proteins like Notch1 and VEGF underlining its critical involvement in pathological angiogenesis and vessel formation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the Notch signaling pathway as Notch ligand (PubMed : 11134954). Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting (PubMed : 20616313). Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (By similarity). During spinal cord neurogenesis, inhibits V2a interneuron fate (PubMed : 17728344).
See full target information DLL4
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of advanced research 68:429-444 PubMed38417575

2024

Salidroside promotes pro-angiogenesis and repair of blood brain barrier via Notch/ITGB1 signal path in CSVD Model.

Applications

Unspecified application

Species

Unspecified reactive species

Tu Zhilan,Zhang Zengyu,Jin Pengpeng,Yang Hualan,Li Chao,Xi Yan,Guo Zimin,Hou Shuangxing,Li Weiwei

Environmental toxicology 38:1628-1640 PubMed36988346

2023

Di-(2-ethylhexyl) phthalate (DEHP) promoted hepatic lipid accumulation by activating Notch signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuezhu Zhang,Honghao Qian,Jia Wang,Ying Zhu,Xiaohan Miao,Xu Li,Jianli Yin,Ruxuan Zhang,Jiaming Ye,Chuanyi Huo,Weisen Zhao,Lin Ye

Experimental and therapeutic medicine 22:972 PubMed34335914

2021

Effect of the Notch4/Dll4 signaling pathway in early gestational intrauterine infection on lung development.

Applications

Unspecified application

Species

Unspecified reactive species

Canyang Zhan,Yi Sun,Jiarong Pan,Lihua Chen,Tianming Yuan

Nature 565:505-510 PubMed30651639

2019

Human blood vessel organoids as a model of diabetic vasculopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Reiner A Wimmer,Alexandra Leopoldi,Martin Aichinger,Nikolaus Wick,Brigitte Hantusch,Maria Novatchkova,Jasmin Taubenschmid,Monika Hämmerle,Christopher Esk,Joshua A Bagley,Dominik Lindenhofer,Guibin Chen,Manfred Boehm,Chukwuma A Agu,Fengtang Yang,Beiyuan Fu,Johannes Zuber,Juergen A Knoblich,Dontscho Kerjaschki,Josef M Penninger

Medical science monitor : international medical jo 24:6280-6288 PubMed30196308

2018

Oxymatrine Inhibits Transforming Growth Factor β1 (TGF-β1)-Induced Cardiac Fibroblast-to-Myofibroblast Transformation (FMT) by Mediating the Notch Signaling Pathway In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Linglu Zhao,Yini Xu,Ling Tao,Yu Yang,Xiangchun Shen,Ling Li,Peng Luo

The Journal of pathology 239:139-51 PubMed26957058

2016

Altered ratios of pro- and anti-angiogenic VEGF-A variants and pericyte expression of DLL4 disrupt vascular maturation in infantile haemangioma.

Applications

IHC-P

Species

Human

Xi Ye,Yassir Abou-Rayyah,Joyce Bischoff,Alison Ritchie,Neil J Sebire,Patrick Watts,Amanda J Churchill,David O Bates
View all publications
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