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Mouse Monoclonal DLX-3 antibody. Suitable for IHC - Wmt and reacts with Zebrafish samples.


Images

IHC - Wholemount - Anti-DLX-3 antibody [Anti-Dlx3b] (AB174436), expandable thumbnail

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: 99% HEPES buffered saline

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC - Wmt
Zebrafish
Tested

Tested
Tested

Species

Zebrafish

Dilution info

1/100

Notes

-

Target data

Function

Involved in the development of the inner ear.

Alternative names

dlx3, dlx3b, Homeobox protein Dlx3b, DLX-3, Distal-less homeobox protein 3b

Recommended products

Mouse Monoclonal DLX-3 antibody. Suitable for IHC - Wmt and reacts with Zebrafish samples.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

Anti-Dlx3b

Light chain type

kappa

Concentration
Loading...
Purification notes

ab174436 is produced in vitro using hybridomas grown in serum-free medium, and then concentrated by chemical fractionation. Near homogeneity as judged by SDS-PAGE.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product was previously marketed under the MitoSciences sub-brand.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

DLX-3 also known as Distal-less homeobox 3 is a transcription factor with a molecular weight of approximately 32 kDa. It is a member of the homeobox gene family which plays roles in developmental processes. DLX-3 is mainly expressed in tissues like skin hair follicles and the placenta. It functions by binding to specific DNA sequences causing changes in gene expression during development.

Biological function summary

DLX-3 affects the development and differentiation processes in organisms. It interacts within a complex network of other homeobox genes influencing the development of craniofacial structures and skin. During early stages its role includes promoting cell differentiation necessary for forming specific tissues particularly in the ectoderm layer derivatives such as skin.

Pathways

Several genes contribute to the pathways involving DLX-3. DLX-3 participates in the Wnt signaling and BMP pathways acting as a regulator of gene transcription necessary for cellular differentiation. It interacts with other proteins like BMP2 showing functional overlap with other members of the DLX gene family. Through these interactions DLX-3 helps coordinate proper tissue development and maintenance.

Associated diseases and disorders

DLX-3 has associations with ectodermal dysplasias and certain craniofacial anomalies. It links to disorders like Tricho–Dento–Osseous (TDO) syndrome where mutations lead to defects in hair teeth and bone development. Apart from these DLX-3’s interactions with MSX1 highlight its role in dental and cranial disorders contributing to further understanding of hereditary conditions affecting developmental processes.

Product promise

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1 product image

  • IHC - Wholemount - Anti-DLX-3 antibody [Anti-Dlx3b] (ab174436), expandable thumbnail

    IHC - Wholemount - Anti-DLX-3 antibody [Anti-Dlx3b] (ab174436)

    ab174436 staining DLX-3 in 19 somite zebrafish by IHC-wholemount. The embryo was fixed with 4% PFA at 4°C over night and an antigen retrieval step was performed with Tris-HCl pH 9. Blocking of the sample was done with 10% sheep serum in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab174436 at 1/100 in blocking solution for 16h at 4°C. An alexa 488 conjugated goat anti-mouse polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. The forebrain is stained by Dlx3b (in green).

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Product protocols

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