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AB174436

Anti-DLX-3 antibody [Anti-Dlx3b]

5

(2 Reviews)

|

(1 Publication)

Mouse Monoclonal DLX-3 antibody. Suitable for IHC - Wmt and reacts with Zebrafish samples. Cited in 1 publication.

View Alternative Names

dlx3, dlx3b, Homeobox protein Dlx3b, DLX-3, Distal-less homeobox protein 3b

1 Images
IHC - Wholemount - Anti-DLX-3 antibody [Anti-Dlx3b] (AB174436)
  • IHC - Wmt

Collaborator

IHC - Wholemount - Anti-DLX-3 antibody [Anti-Dlx3b] (AB174436)

ab174436 staining DLX-3 in 19 somite zebrafish by IHC-wholemount. The embryo was fixed with 4% PFA at 4°C over night and an antigen retrieval step was performed with Tris-HCl pH 9. Blocking of the sample was done with 10% sheep serum in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab174436 at 1/100 in blocking solution for 16h at 4°C. An alexa 488 conjugated goat anti-mouse polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. The forebrain is stained by Dlx3b (in green).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Anti-Dlx3b

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Zebrafish

Applications

IHC - Wmt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCWmt" : {"fullname" : "IHC - Wholemount", "shortname":"IHC - Wmt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Zebrafish": { "IHCWmt-species-checked": "testedAndGuaranteed", "IHCWmt-species-dilution-info": "1/100", "IHCWmt-species-notes": "<p></p>" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

Form
Liquid
Purity
undefined SDS-PAGE
Purification notes
ab174436 is produced in vitro using hybridomas grown in serum-free medium, and then concentrated by chemical fractionation. Near homogeneity as judged by SDS-PAGE.
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: 99% HEPES buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DLX-3 also known as Distal-less homeobox 3 is a transcription factor with a molecular weight of approximately 32 kDa. It is a member of the homeobox gene family which plays roles in developmental processes. DLX-3 is mainly expressed in tissues like skin hair follicles and the placenta. It functions by binding to specific DNA sequences causing changes in gene expression during development.
Biological function summary

DLX-3 affects the development and differentiation processes in organisms. It interacts within a complex network of other homeobox genes influencing the development of craniofacial structures and skin. During early stages its role includes promoting cell differentiation necessary for forming specific tissues particularly in the ectoderm layer derivatives such as skin.

Pathways

Several genes contribute to the pathways involving DLX-3. DLX-3 participates in the Wnt signaling and BMP pathways acting as a regulator of gene transcription necessary for cellular differentiation. It interacts with other proteins like BMP2 showing functional overlap with other members of the DLX gene family. Through these interactions DLX-3 helps coordinate proper tissue development and maintenance.

DLX-3 has associations with ectodermal dysplasias and certain craniofacial anomalies. It links to disorders like Tricho–Dento–Osseous (TDO) syndrome where mutations lead to defects in hair teeth and bone development. Apart from these DLX-3’s interactions with MSX1 highlight its role in dental and cranial disorders contributing to further understanding of hereditary conditions affecting developmental processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the development of the inner ear.
See full target information dlx3b
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 66:67 PubMed40266594

2025

Microglia-Derived IL-6 Promotes Müller Glia Reprogramming and Proliferation in Zebrafish Retina Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Xu,Yi Li,Xiangyu Li,Xuan Tan,Lihua Liu,Lining Cao,Hui Xu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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