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AB308209

Anti-DLX2 antibody [HL1942]

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Rabbit Recombinant Monoclonal DLX2 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human DLX2.

View Alternative Names

Homeobox protein DLX-2, DLX2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-DLX2 antibody [HL1942] (AB308209)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DLX2 antibody [HL1942] (AB308209)

Immunofluorescent analysis of 4% paraformaldehyde-fixed DIV9 rat E18 primary cortical neuron and glia cells labeling DLX2 with ab308209 at 1/250 dilution (Green). Red : Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1/500. Blue : Fluoroshield with DAPI.

Western blot - Anti-DLX2 antibody [HL1942] (AB308209)
  • WB

Supplier Data

Western blot - Anti-DLX2 antibody [HL1942] (AB308209)

293T whole cell and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DLX2 antibody [HL1942] (ab308209) diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (WCE : whole cell extract; NE : nuclear extract)

Lane 1:

293T whole cell extract at 30 µg

Lane 2:

293T cell nuclear extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-DLX2 antibody [HL1942] (AB308209)
  • WB

Supplier Data

Western blot - Anti-DLX2 antibody [HL1942] (AB308209)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DLX2 antibody [HL1942] (ab308209) diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Lane 1:

Non-transfected 293T whole cell extracts at 30 µg

Lane 2:

DLX2-transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1942

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Rat

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human DLX2. The exact immunogen used to generate this antibody is proprietary information.

Q07687

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a transcriptional activator (By similarity). Activates transcription of CGA/alpha-GSU, via binding to the downstream activin regulatory element (DARE) in the gene promoter (By similarity). Plays a role in terminal differentiation of interneurons, such as amacrine and bipolar cells in the developing retina. Likely to play a regulatory role in the development of the ventral forebrain (By similarity). May play a role in craniofacial patterning and morphogenesis (By similarity).
See full target information DLX2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com