Rabbit Polyclonal DLX3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human Homeobox protein DLX-3.
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Publications
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- Molecular medicine reports 23:2021Effects of DLX3 on the osteogenic differentiation of induced pluripotent stem cell‑derived mesenchymal stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunyuan Li,Qiang Lin,Yixin Lin,Renfa Lai,Wen ZhangPubMed 33655330
- Scientific reports 7:107752017BMP-2 induced Dspp transcription is mediated by Dlx3/Osx signaling pathway in odontoblasts.Applications:ICC/IFReactive species:MouseGuobin Yang,Guohua Yuan,Mary MacDougall,Chen Zhi,Shuo ChenPubMed 28883412
- Journal of cellular physiology 231:1189-982015Establishment of Immortalized BMP2/4 Double Knock-Out Osteoblastic Cells Is Essential for Study of Osteoblast Growth, Differentiation, and Osteogenesis.Applications:WBReactive species:MouseLi-An Wu,Feng Wang,Kevin J Donly,Andrew Baker,Chunyan Wan,Daoshu Luo,Mary MacDougall,Shuo ChenPubMed 26595646
- PloS one 10:e01212882015Transcriptional factor DLX3 promotes the gene expression of enamel matrix proteins during amelogenesis.
- PloS one 9:e1038612014Nicotinic receptor Alpha7 expression during mouse adrenal gland development.Applications:IHC-FoFrReactive species:MouseLorise C Gahring,Elizabeth Myers,Sierra Palumbos,Scott W RogersPubMed 25093893
- Cell and tissue research 343:545-582011Development and characterization of a mouse floxed Bmp2 osteoblast cell line that retains osteoblast genotype and phenotype.Applications:ICC/IFReactive species:MouseLi-an Wu,Junsheng Feng,Lynn Wang,Yan-dong Mu,Andrew Baker,Kevin J Donly,Stephen E Harris,Mary MacDougall,Shuo ChenPubMed 21271257
- Journal of cellular physiology 225:132-92010Immortalized mouse floxed Bmp2 dental papilla mesenchymal cell lines preserve odontoblastic phenotype and respond to BMP2.Applications:ICC/IFReactive species:MouseLi-an Wu,Junsheng Feng,Lynn Wang,Yan-dong Mu,Andrew Baker,Kevin J Donly,Jelica Gluhak-Heinrich,Stephen E Harris,Mary MacDougall,Shuo ChenPubMed 20458728
- Biochemical and biophysical research communications 386:89-952009Immortalization and characterization of mouse floxed Bmp2/4 osteoblasts.Applications:ICC/IFReactive species:MouseLi-An Wu,Guohua Yuan,Guobin Yang,Iris Ortiz-Gonzalez,Wuchen Yang,Yong Cui,Mary MacDougall,Kevin J Donly,Stephen Harris,Shuo ChenPubMed 19501574
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human Homeobox protein DLX-3. The exact immunogen used to generate this antibody is proprietary information. Database link O60479
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Transcriptional activator (By similarity). Activates transcription of GNRHR, via binding to the downstream activin regulatory element (DARE) in the gene promoter (By similarity).
Alternative names
Homeobox protein DLX-3, DLX3
Recommended products
Rabbit Polyclonal DLX3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human Homeobox protein DLX-3.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human Homeobox protein DLX-3. The exact immunogen used to generate this antibody is proprietary information. Database link O60479
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DLX3 also known as Distal-less homeobox 3 or BPES3 functions mechanically as a transcription factor with a molecular weight of approximately 30 kDa. This protein localizes mainly to the nucleus and is an essential regulator of gene expression. It is observed in tissues such as the epidermis placenta and developing teeth. By binding to specific DNA sequences DLX3 influences the transcriptional activity of various genes important for development.
- Biological function summary
DLX3 participates in the regulation of cell differentiation processes during development. It acts individually and as part of a larger protein complex that influences the development of craniofacial structures and appendages by controlling gene expression in these areas. Its expression patterns suggest that it plays a vital role in morphogenesis and tissue specificity contributing to the development and compartmentalization within tissues.
- Pathways
DLX3 interacts with key developmental signaling cascades notably the Wnt and BMP (Bone Morphogenetic Protein) pathways. These pathways regulate cellular differentiation and morphogenesis. In these pathways DLX3 indirectly interacts with proteins such as LEF1 and SMAD which are themselves involved in mediating the downstream effects of Wnt and BMP signaling. DLX3's role in these pathways highlights its importance in coordinating gene regulatory networks that drive organogenesis and development.
- Associated diseases and disorders
DLX3 mutations are associated with Tricho-Dento-Osseous (TDO) syndrome and amelogenesis imperfecta affecting hair teeth and bone structure. DLX3 interacts with proteins like MSX1 and RUNX2 in the pathogenesis of these conditions which are characterized by defects in craniofacial and dental development. Understanding these interactions allows for a better comprehension of the molecular mechanisms underlying these disorders and potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLX3 antibody (ab64953)
Immunohistochemistry analysis of paraffin-embedded human heart tissue using ab64953 at 1/50 dilution. Right hand panel was also treated with immunising peptide.
Western blot - Anti-DLX3 antibody (ab64953)
All lanes: Western blot - Anti-DLX3 antibody (ab64953) at 1/500 dilution
Lane 1: 293 cell extract at 5 µg
Lane 2: 293 cell extract at 5 µg with immunising peptide
Predicted band size: 32 kDa
Observed band size: 40 kDa, 60 kDa
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