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AB64953

Anti-DLX3 antibody

1

(1 Review)

|

(8 Publications)

Rabbit Polyclonal DLX3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human Homeobox protein DLX-3.

View Alternative Names

Homeobox protein DLX-3, DLX3

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLX3 antibody (AB64953)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLX3 antibody (AB64953)

Immunohistochemistry analysis of paraffin-embedded human heart tissue using ab64953 at 1/50 dilution. Right hand panel was also treated with immunising peptide.

Western blot - Anti-DLX3 antibody (AB64953)
  • WB

Unknown

Western blot - Anti-DLX3 antibody (AB64953)

All lanes:

Western blot - Anti-DLX3 antibody (ab64953) at 1/500 dilution

Lane 1:

293 cell extract at 5 µg

Lane 2:

293 cell extract at 5 µg with immunising peptide

Predicted band size: 32 kDa

Observed band size: 40 kDa,60 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human Homeobox protein DLX-3. The exact immunogen used to generate this antibody is proprietary information.

O60479

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DLX3 also known as Distal-less homeobox 3 or BPES3 functions mechanically as a transcription factor with a molecular weight of approximately 30 kDa. This protein localizes mainly to the nucleus and is an essential regulator of gene expression. It is observed in tissues such as the epidermis placenta and developing teeth. By binding to specific DNA sequences DLX3 influences the transcriptional activity of various genes important for development.
Biological function summary

DLX3 participates in the regulation of cell differentiation processes during development. It acts individually and as part of a larger protein complex that influences the development of craniofacial structures and appendages by controlling gene expression in these areas. Its expression patterns suggest that it plays a vital role in morphogenesis and tissue specificity contributing to the development and compartmentalization within tissues.

Pathways

DLX3 interacts with key developmental signaling cascades notably the Wnt and BMP (Bone Morphogenetic Protein) pathways. These pathways regulate cellular differentiation and morphogenesis. In these pathways DLX3 indirectly interacts with proteins such as LEF1 and SMAD which are themselves involved in mediating the downstream effects of Wnt and BMP signaling. DLX3's role in these pathways highlights its importance in coordinating gene regulatory networks that drive organogenesis and development.

DLX3 mutations are associated with Tricho-Dento-Osseous (TDO) syndrome and amelogenesis imperfecta affecting hair teeth and bone structure. DLX3 interacts with proteins like MSX1 and RUNX2 in the pathogenesis of these conditions which are characterized by defects in craniofacial and dental development. Understanding these interactions allows for a better comprehension of the molecular mechanisms underlying these disorders and potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional activator (By similarity). Activates transcription of GNRHR, via binding to the downstream activin regulatory element (DARE) in the gene promoter (By similarity).
See full target information Homeobox protein DLX-3
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 23: PubMed33655330

2021

Effects of DLX3 on the osteogenic differentiation of induced pluripotent stem cell‑derived mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Junyuan Li,Qiang Lin,Yixin Lin,Renfa Lai,Wen Zhang

Scientific reports 7:10775 PubMed28883412

2017

BMP-2 induced Dspp transcription is mediated by Dlx3/Osx signaling pathway in odontoblasts.

Applications

ICC/IF

Species

Mouse

Guobin Yang,Guohua Yuan,Mary MacDougall,Chen Zhi,Shuo Chen

Journal of cellular physiology 231:1189-98 PubMed26595646

2015

Establishment of Immortalized BMP2/4 Double Knock-Out Osteoblastic Cells Is Essential for Study of Osteoblast Growth, Differentiation, and Osteogenesis.

Applications

WB

Species

Mouse

Li-An Wu,Feng Wang,Kevin J Donly,Andrew Baker,Chunyan Wan,Daoshu Luo,Mary MacDougall,Shuo Chen

PloS one 10:e0121288 PubMed25815730

2015

Transcriptional factor DLX3 promotes the gene expression of enamel matrix proteins during amelogenesis.

Applications

IHC-P

Species

Unspecified reactive species

Zhichun Zhang,Hua Tian,Ping Lv,Weiping Wang,Zhuqing Jia,Sainan Wang,Chunyan Zhou,Xuejun Gao

PloS one 9:e103861 PubMed25093893

2014

Nicotinic receptor Alpha7 expression during mouse adrenal gland development.

Applications

IHC-FoFr

Species

Mouse

Lorise C Gahring,Elizabeth Myers,Sierra Palumbos,Scott W Rogers

Cell and tissue research 343:545-58 PubMed21271257

2011

Development and characterization of a mouse floxed Bmp2 osteoblast cell line that retains osteoblast genotype and phenotype.

Applications

ICC/IF

Species

Mouse

Li-an Wu,Junsheng Feng,Lynn Wang,Yan-dong Mu,Andrew Baker,Kevin J Donly,Stephen E Harris,Mary MacDougall,Shuo Chen

Journal of cellular physiology 225:132-9 PubMed20458728

2010

Immortalized mouse floxed Bmp2 dental papilla mesenchymal cell lines preserve odontoblastic phenotype and respond to BMP2.

Applications

ICC/IF

Species

Mouse

Li-an Wu,Junsheng Feng,Lynn Wang,Yan-dong Mu,Andrew Baker,Kevin J Donly,Jelica Gluhak-Heinrich,Stephen E Harris,Mary MacDougall,Shuo Chen

Biochemical and biophysical research communications 386:89-95 PubMed19501574

2009

Immortalization and characterization of mouse floxed Bmp2/4 osteoblasts.

Applications

ICC/IF

Species

Mouse

Li-An Wu,Guohua Yuan,Guobin Yang,Iris Ortiz-Gonzalez,Wuchen Yang,Yong Cui,Mary MacDougall,Kevin J Donly,Stephen Harris,Shuo Chen
View all publications
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Product promise

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