JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB178428

Anti-DLX3 antibody [EPR8762(2)]

Be the first to review this product! Submit a review

|

(5 Publications)

Rabbit Recombinant Monoclonal DLX3 antibody. Suitable for IP, WB and reacts with Human, Rat samples. Cited in 5 publications.

View Alternative Names

Homeobox protein DLX-3, DLX3

4 Images
Immunoprecipitation - Anti-DLX3 antibody [EPR8762(2)] (AB178428)
  • IP

Lab

Immunoprecipitation - Anti-DLX3 antibody [EPR8762(2)] (AB178428)

DLX3 was immunoprecipitated from Mouse skin tissue lysate with ab178428 at 1/50 dilution (2μg in 0.35mg lysate). Western blot was performed on the immunoprecipitate using ab178428 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution. Lane 1 : Mouse skin tissue lysate 10μg Lane 2 : abab178428 IP in Mouse skin tissue lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab178428 in Mouse skin tissue lysate. Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 67 seconds.

Western blot - Anti-DLX3 antibody [EPR8762(2)] (AB178428)
  • WB

Supplier Data

Western blot - Anti-DLX3 antibody [EPR8762(2)] (AB178428)

All lanes:

Western blot - Anti-DLX3 antibody [EPR8762(2)] (ab178428) at 1/1000 dilution

Lane 1:

JAR cell line lysate at 10 µg

Lane 2:

Human skin tissue lysate at 10 µg

Lane 3:

Rat skin tissue lysate at 10 µg

Lane 4:

293T cell line lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody

Predicted band size: 32 kDa

true

Western blot - Anti-DLX3 antibody [EPR8762(2)] (AB178428)
  • WB

Lab

Western blot - Anti-DLX3 antibody [EPR8762(2)] (AB178428)

Blocking buffer and concentration : 5% NFDM/TBST Exposure time : Lane 1 : 7 seconds, Lane 2-5 : 40 seconds

All lanes:

Western blot - Anti-DLX3 antibody [EPR8762(2)] (ab178428) at 1/1000 dilution

Lane 1:

Mouse skin tissue lysate at 20 µg

Lane 2:

Rat skin tissue lysate at 20 µg

Lane 3:

HaCaT (Human skin keratinocyte cell) whole cell lysate at 20 µg

Lane 4:

JAR (Human placenta choriocarcinoma epithelial cell) (RIPA) whole cell lysate at 20 µg

Lane 5:

JAR (Human placenta choriocarcinoma epithelial cell) (HOT) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 32 kDa

Observed band size: 40 kDa

false

Immunoprecipitation - Anti-DLX3 antibody [EPR8762(2)] (AB178428)
  • IP

Supplier Data

Immunoprecipitation - Anti-DLX3 antibody [EPR8762(2)] (AB178428)

Western blot analysis on immunoprecipitation pellet from (1) Human fetal skin lysate or (2) 1XPBS (negative control) immunoprecipitated using ab178428 with HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-DLX3 antibody [EPR8762(2)] (ab178428)

Predicted band size: 32 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8762(2)

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

This antibody was developed through collaboration with the lab of Maria Morasso at the National Cancer Institute, NIH.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DLX3 also known as Distal-less homeobox 3 or BPES3 functions mechanically as a transcription factor with a molecular weight of approximately 30 kDa. This protein localizes mainly to the nucleus and is an essential regulator of gene expression. It is observed in tissues such as the epidermis placenta and developing teeth. By binding to specific DNA sequences DLX3 influences the transcriptional activity of various genes important for development.
Biological function summary

DLX3 participates in the regulation of cell differentiation processes during development. It acts individually and as part of a larger protein complex that influences the development of craniofacial structures and appendages by controlling gene expression in these areas. Its expression patterns suggest that it plays a vital role in morphogenesis and tissue specificity contributing to the development and compartmentalization within tissues.

Pathways

DLX3 interacts with key developmental signaling cascades notably the Wnt and BMP (Bone Morphogenetic Protein) pathways. These pathways regulate cellular differentiation and morphogenesis. In these pathways DLX3 indirectly interacts with proteins such as LEF1 and SMAD which are themselves involved in mediating the downstream effects of Wnt and BMP signaling. DLX3's role in these pathways highlights its importance in coordinating gene regulatory networks that drive organogenesis and development.

DLX3 mutations are associated with Tricho-Dento-Osseous (TDO) syndrome and amelogenesis imperfecta affecting hair teeth and bone structure. DLX3 interacts with proteins like MSX1 and RUNX2 in the pathogenesis of these conditions which are characterized by defects in craniofacial and dental development. Understanding these interactions allows for a better comprehension of the molecular mechanisms underlying these disorders and potential therapeutic targets.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Transcriptional activator (By similarity). Activates transcription of GNRHR, via binding to the downstream activin regulatory element (DARE) in the gene promoter (By similarity).
See full target information Homeobox protein DLX-3
style
left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in endocrinology 12:703167 PubMed34925225

2021

The miR-4739/DLX3 Axis Modulates Bone Marrow-Derived Mesenchymal Stem Cell (BMSC) Osteogenesis Affecting Osteoporosis Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Ding Li,Qi Yuan,Liang Xiong,Aoyu Li,Yu Xia

Oncogene 40:3680-3694 PubMed33947961

2021

Loss of DLX3 tumor suppressive function promotes progression of SCC through EGFR-ERBB2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Deepti Bajpai,Spencer Mehdizadeh,Akihiko Uchiyama,Yuta Inoue,Andrew Sawaya,Andrew Overmiller,Stephen R Brooks,Kowser Hasneen,Meghan Kellett,Elisabetta Palazzo,Sei-Ichiro Motegi,Stuart H Yuspa,Christophe Cataisson,Maria I Morasso

Cell stem cell 28:257-272.e11 PubMed33091368

2020

Non-canonical Targets of HIF1a Impair Oligodendrocyte Progenitor Cell Function.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin C Allan,Lucille R Hu,Marissa A Scavuzzo,Andrew R Morton,Artur S Gevorgyan,Erin F Cohn,Benjamin L L Clayton,Ilya R Bederman,Stevephen Hung,Cynthia F Bartels,Mayur Madhavan,Paul J Tesar

The Journal of investigative dermatology 138:1052-1061 PubMed29246798

2017

DLX3-Dependent STAT3 Signaling in Keratinocytes Regulates Skin Immune Homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Shreya Bhattacharya,Jin-Chul Kim,Youichi Ogawa,Gaku Nakato,Veronica Nagle,Stephen R Brooks,Mark C Udey,Maria I Morasso

Cell death and differentiation 24:717-730 PubMed28186503

2017

A novel DLX3-PKC integrated signaling network drives keratinocyte differentiation.

Applications

ChIP

Species

Unspecified reactive species

Elisabetta Palazzo,Meghan D Kellett,Christophe Cataisson,Paul W Bible,Shreya Bhattacharya,Hong-Wei Sun,Anna C Gormley,Stuart H Yuspa,Maria I Morasso
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB211492

Anti-DLX3 antibody [NCI-R183-14]

RabMAb|Recombinant

Western blot - Anti-DLX3 antibody [NCI-R183-14] (AB211492)

  • 2
  • 1
Primary Antibodies

AB64953

Anti-DLX3 antibody

Western blot - Anti-DLX3 antibody (AB64953)

  • 1
  • 1

Complementary Products

Primary Antibodies

AB22552

Anti-Sp7 / Osterix antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

  • 5
  • 24
Primary Antibodies

AB192256

Anti-RUNX2 antibody [EPR14334]

Recombinant|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX2 antibody [EPR14334] (AB192256)

  • 5
  • 8
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB272276

Anti-Osteocalcin antibody [EPR22338-92] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Osteocalcin antibody [EPR22338-92] - BSA and Azide free (Detector) (AB272276)

  • 4
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com