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AB250049

Anti-DLX3 antibody [EPR8762(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal DLX3 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Rat samples.

View Alternative Names

Homeobox protein DLX-3, DLX3

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Immunoprecipitation - Anti-DLX3 antibody [EPR8762(2)] - BSA and Azide free (AB250049)
  • IP

Supplier Data

Immunoprecipitation - Anti-DLX3 antibody [EPR8762(2)] - BSA and Azide free (AB250049)

This data was developed using ab178428, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from (1) Human fetal skin lysate or (2) 1XPBS (negative control) immunoprecipitated using ab178428 with HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-DLX3 antibody [EPR8762(2)] (<a href='/en-us/products/primary-antibodies/dlx3-antibody-epr87622-ab178428'>ab178428</a>)

Predicted band size: 32 kDa

true

Immunoprecipitation - Anti-DLX3 antibody [EPR8762(2)] - BSA and Azide free (AB250049)
  • IP

Lab

Immunoprecipitation - Anti-DLX3 antibody [EPR8762(2)] - BSA and Azide free (AB250049)

This data was developed using ab178428, the same antibody clone in a different buffer formulation.

DLX3 was immunoprecipitated from Mouse skin tissue lysate with ab178428 at 1/50 dilution (2μg in 0.35mg lysate). Western blot was performed on the immunoprecipitate using ab178428 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

Lane 1 : Mouse skin tissue lysate 10μg
Lane 2 : ab178428 IP in Mouse skin tissue lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab178428 in Mouse skin tissue lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 67 seconds.

Western blot - Anti-DLX3 antibody [EPR8762(2)] - BSA and Azide free (AB250049)
  • WB

Supplier Data

Western blot - Anti-DLX3 antibody [EPR8762(2)] - BSA and Azide free (AB250049)

This data was developed using ab178428, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-DLX3 antibody [EPR8762(2)] (<a href='/en-us/products/primary-antibodies/dlx3-antibody-epr87622-ab178428'>ab178428</a>) at 1/1000 dilution

Lane 1:

JAR cell line lysate at 10 µg

Lane 2:

Human skin tissue lysate at 10 µg

Lane 3:

Rat skin tissue lysate at 10 µg

Lane 4:

293T cell line lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody

Predicted band size: 32 kDa

true

Western blot - Anti-DLX3 antibody [EPR8762(2)] - BSA and Azide free (AB250049)
  • WB

Lab

Western blot - Anti-DLX3 antibody [EPR8762(2)] - BSA and Azide free (AB250049)

All lanes:

Western blot - Anti-DLX3 antibody [EPR8762(2)] (<a href='/en-us/products/primary-antibodies/dlx3-antibody-epr87622-ab178428'>ab178428</a>) at 1/1000 dilution

Lane 1:

Mouse skin tissue lysate at 20 µg

Lane 2:

Rat skin tissue lysate at 20 µg

Lane 3:

HaCaT (Human skin keratinocyte cell) whole cell lysate at 20 µg

Lane 4:

JAR (Human placenta choriocarcinoma epithelial cell) (RIPA) whole cell lysate at 20 µg

Lane 5:

JAR (Human placenta choriocarcinoma epithelial cell) (HOT) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 32 kDa

Observed band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8762(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab250049 is the carrier-free version of ab178428.

This antibody was developed through collaboration with the lab of Maria Morasso at the National Cancer Institute, NIH.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DLX3 also known as Distal-less homeobox 3 or BPES3 functions mechanically as a transcription factor with a molecular weight of approximately 30 kDa. This protein localizes mainly to the nucleus and is an essential regulator of gene expression. It is observed in tissues such as the epidermis placenta and developing teeth. By binding to specific DNA sequences DLX3 influences the transcriptional activity of various genes important for development.
Biological function summary

DLX3 participates in the regulation of cell differentiation processes during development. It acts individually and as part of a larger protein complex that influences the development of craniofacial structures and appendages by controlling gene expression in these areas. Its expression patterns suggest that it plays a vital role in morphogenesis and tissue specificity contributing to the development and compartmentalization within tissues.

Pathways

DLX3 interacts with key developmental signaling cascades notably the Wnt and BMP (Bone Morphogenetic Protein) pathways. These pathways regulate cellular differentiation and morphogenesis. In these pathways DLX3 indirectly interacts with proteins such as LEF1 and SMAD which are themselves involved in mediating the downstream effects of Wnt and BMP signaling. DLX3's role in these pathways highlights its importance in coordinating gene regulatory networks that drive organogenesis and development.

DLX3 mutations are associated with Tricho-Dento-Osseous (TDO) syndrome and amelogenesis imperfecta affecting hair teeth and bone structure. DLX3 interacts with proteins like MSX1 and RUNX2 in the pathogenesis of these conditions which are characterized by defects in craniofacial and dental development. Understanding these interactions allows for a better comprehension of the molecular mechanisms underlying these disorders and potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional activator (By similarity). Activates transcription of GNRHR, via binding to the downstream activin regulatory element (DARE) in the gene promoter (By similarity).
See full target information Homeobox protein DLX-3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com