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Rabbit Recombinant Monoclonal DLX3 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Rat samples.

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Images

Western blot - Anti-DLX3 antibody [EPR8762(2)] - BSA and Azide free (AB250049), expandable thumbnail
  • Immunoprecipitation - Anti-DLX3 antibody [EPR8762(2)] - BSA and Azide free (AB250049), expandable thumbnail
  • Western blot - Anti-DLX3 antibody [EPR8762(2)] - BSA and Azide free (AB250049), expandable thumbnail
  • Immunoprecipitation - Anti-DLX3 antibody [EPR8762(2)] - BSA and Azide free (AB250049), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPIHCFlow CytWBICC/IF
Human
Tested
Not recommended
Not recommended
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Expected
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Target data

Function

Transcriptional activator (By similarity). Activates transcription of GNRHR, via binding to the downstream activin regulatory element (DARE) in the gene promoter (By similarity).

Alternative names

Homeobox protein DLX-3, DLX3

Recommended products

Rabbit Recombinant Monoclonal DLX3 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR8762(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab250049 is the carrier-free version of Anti-DLX3 antibody [EPR8762(2)] ab178428.

This antibody was developed through collaboration with the lab of Maria Morasso at the National Cancer Institute, NIH.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

DLX3 also known as Distal-less homeobox 3 or BPES3 functions mechanically as a transcription factor with a molecular weight of approximately 30 kDa. This protein localizes mainly to the nucleus and is an essential regulator of gene expression. It is observed in tissues such as the epidermis placenta and developing teeth. By binding to specific DNA sequences DLX3 influences the transcriptional activity of various genes important for development.

Biological function summary

DLX3 participates in the regulation of cell differentiation processes during development. It acts individually and as part of a larger protein complex that influences the development of craniofacial structures and appendages by controlling gene expression in these areas. Its expression patterns suggest that it plays a vital role in morphogenesis and tissue specificity contributing to the development and compartmentalization within tissues.

Pathways

DLX3 interacts with key developmental signaling cascades notably the Wnt and BMP (Bone Morphogenetic Protein) pathways. These pathways regulate cellular differentiation and morphogenesis. In these pathways DLX3 indirectly interacts with proteins such as LEF1 and SMAD which are themselves involved in mediating the downstream effects of Wnt and BMP signaling. DLX3's role in these pathways highlights its importance in coordinating gene regulatory networks that drive organogenesis and development.

Associated diseases and disorders

DLX3 mutations are associated with Tricho-Dento-Osseous (TDO) syndrome and amelogenesis imperfecta affecting hair teeth and bone structure. DLX3 interacts with proteins like MSX1 and RUNX2 in the pathogenesis of these conditions which are characterized by defects in craniofacial and dental development. Understanding these interactions allows for a better comprehension of the molecular mechanisms underlying these disorders and potential therapeutic targets.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-DLX3 antibody [EPR8762(2)] - BSA and Azide free (ab250049), expandable thumbnail

    Western blot - Anti-DLX3 antibody [EPR8762(2)] - BSA and Azide free (ab250049)

    This data was developed using Anti-DLX3 antibody [EPR8762(2)] ab178428, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-DLX3 antibody [EPR8762(2)] (Anti-DLX3 antibody [EPR8762(2)] ab178428) at 1/1000 dilution

    Lane 1: JAR cell line lysate at 10 µg

    Lane 2: Human skin tissue lysate at 10 µg

    Lane 3: Rat skin tissue lysate at 10 µg

    Lane 4: 293T cell line lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP conjugated antibody

    Developed using the ECL technique.

    Predicted band size: 32 kDa

  • Immunoprecipitation - Anti-DLX3 antibody [EPR8762(2)] - BSA and Azide free (ab250049), expandable thumbnail

    Immunoprecipitation - Anti-DLX3 antibody [EPR8762(2)] - BSA and Azide free (ab250049)

    This data was developed using Anti-DLX3 antibody [EPR8762(2)] ab178428, the same antibody clone in a different buffer formulation.

    Western blot analysis on immunoprecipitation pellet from (1) Human fetal skin lysate or (2) 1XPBS (negative control) immunoprecipitated using Anti-DLX3 antibody [EPR8762(2)] ab178428 with HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    All lanes: Immunoprecipitation - Anti-DLX3 antibody [EPR8762(2)] (Anti-DLX3 antibody [EPR8762(2)] ab178428)

    Developed using the ECL technique.

    Predicted band size: 32 kDa

  • Western blot - Anti-DLX3 antibody [EPR8762(2)] - BSA and Azide free (ab250049), expandable thumbnail

    Western blot - Anti-DLX3 antibody [EPR8762(2)] - BSA and Azide free (ab250049)

    All lanes: Western blot - Anti-DLX3 antibody [EPR8762(2)] (Anti-DLX3 antibody [EPR8762(2)] ab178428) at 1/1000 dilution

    Lane 1: Mouse skin tissue lysate at 20 µg

    Lane 2: Rat skin tissue lysate at 20 µg

    Lane 3: HaCaT (Human skin keratinocyte cell) whole cell lysate at 20 µg

    Lane 4: JAR (Human placenta choriocarcinoma epithelial cell) (RIPA) whole cell lysate at 20 µg

    Lane 5: JAR (Human placenta choriocarcinoma epithelial cell) (HOT) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 32 kDa

    Observed band size: 40 kDa

  • Immunoprecipitation - Anti-DLX3 antibody [EPR8762(2)] - BSA and Azide free (ab250049), expandable thumbnail

    Immunoprecipitation - Anti-DLX3 antibody [EPR8762(2)] - BSA and Azide free (ab250049)

    This data was developed using Anti-DLX3 antibody [EPR8762(2)] ab178428, the same antibody clone in a different buffer formulation.

    DLX3 was immunoprecipitated from Mouse skin tissue lysate with Anti-DLX3 antibody [EPR8762(2)] ab178428 at 1/50 dilution (2μg in 0.35mg lysate). Western blot was performed on the immunoprecipitate using Anti-DLX3 antibody [EPR8762(2)] ab178428 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution.

    Lane 1: Mouse skin tissue lysate 10μg

    Lane 2: abab178428 IP in Mouse skin tissue lysate.

    Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-DLX3 antibody [EPR8762(2)] ab178428 in Mouse skin tissue lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 67 seconds.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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