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AB211492

Anti-DLX3 antibody [NCI-R183-14]

2

(1 Review)

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(1 Publication)

Rabbit Recombinant Monoclonal DLX3 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Homeobox protein DLX-3, DLX3

1 Images
Western blot - Anti-DLX3 antibody [NCI-R183-14] (AB211492)
  • WB

Supplier Data

Western blot - Anti-DLX3 antibody [NCI-R183-14] (AB211492)

Blocking/Dilution buffer : 5% NFDM/TBST.

This product only recognizes DLX3 carrying the TDO (Tricho-Dento-Osseous syndrome) frameshift mutation.

All lanes:

Western blot - Anti-DLX3 antibody [NCI-R183-14] (ab211492) at 1/1000 dilution

Lane 1:

Saos-2 (Human osteosarcoma cell line) cell transfected with mouse TetOFFv5DLX3/flag TDO treated with DOX(Doxorubicin 2µg/ml)

Lane 2:

Saos-2 (Human osteosarcoma cell line) cell transfected with mouse TetOFFv5DLX3/flag TDO untreated with DOX(Doxorubicin 2μg/ml)

Lane 3:

Saos-2 (Human osteosarcoma cell line) cell transfected with human TetOFFv5DLX3/flag TDO treated with DOX(Doxorubicin 2μg/ml)

Lane 4:

Saos-2 (Human osteosarcoma cell line) cell transfected with human TetOFFv5DLX3/flag TDO untreated with DOX(Doxorubicin 2μg/ml)

Predicted band size: 32 kDa

Observed band size: 38 kDa,45 kDa

false

Exposure time: 30s

The data was kindly provided by Dr. Maria Morasso (NIH/NIAMS).

  • Carrier free

    Anti-DLX3 antibody [NCI-R183-14] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

NCI-R183-14

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DLX3 also known as Distal-less homeobox 3 or BPES3 functions mechanically as a transcription factor with a molecular weight of approximately 30 kDa. This protein localizes mainly to the nucleus and is an essential regulator of gene expression. It is observed in tissues such as the epidermis placenta and developing teeth. By binding to specific DNA sequences DLX3 influences the transcriptional activity of various genes important for development.
Biological function summary

DLX3 participates in the regulation of cell differentiation processes during development. It acts individually and as part of a larger protein complex that influences the development of craniofacial structures and appendages by controlling gene expression in these areas. Its expression patterns suggest that it plays a vital role in morphogenesis and tissue specificity contributing to the development and compartmentalization within tissues.

Pathways

DLX3 interacts with key developmental signaling cascades notably the Wnt and BMP (Bone Morphogenetic Protein) pathways. These pathways regulate cellular differentiation and morphogenesis. In these pathways DLX3 indirectly interacts with proteins such as LEF1 and SMAD which are themselves involved in mediating the downstream effects of Wnt and BMP signaling. DLX3's role in these pathways highlights its importance in coordinating gene regulatory networks that drive organogenesis and development.

DLX3 mutations are associated with Tricho-Dento-Osseous (TDO) syndrome and amelogenesis imperfecta affecting hair teeth and bone structure. DLX3 interacts with proteins like MSX1 and RUNX2 in the pathogenesis of these conditions which are characterized by defects in craniofacial and dental development. Understanding these interactions allows for a better comprehension of the molecular mechanisms underlying these disorders and potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional activator (By similarity). Activates transcription of GNRHR, via binding to the downstream activin regulatory element (DARE) in the gene promoter (By similarity).
See full target information Homeobox protein DLX-3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Forensic science, medicine, and pathology 17:486-492 PubMed34191249

2021

Fox sign in a case of terminal stage pancreatic cancer and suggestions for diagnosis.

Applications

Unspecified application

Species

Unspecified reactive species

Julian Prangenberg,Elke Doberentz,Burkhard Madea
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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