AB249869

Anti-Dlx4/BP1 antibody [EPR9263] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Dlx4/BP1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

BP1, DLX7, DLX8, DLX9, DLX4, Homeobox protein DLX-4, Beta protein 1, Homeobox protein DLX-7, Homeobox protein DLX-8

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dlx4/BP1 antibody [EPR9263] - BSA and Azide free (AB249869)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dlx4/BP1 antibody [EPR9263] - BSA and Azide free (AB249869)

This data was developed using ab175218, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Dlx4/BP1 with ab175218 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dlx4/BP1 antibody [EPR9263] - BSA and Azide free (AB249869)

This data was developed using ab175218, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling Dlx4/BP1 with ab175218 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Dlx4/BP1 antibody [EPR9263] - BSA and Azide free (AB249869)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Dlx4/BP1 antibody [EPR9263] - BSA and Azide free (AB249869)

This data was developed using ab175218, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of SW480 cells labeling Dlx4/BP1 with ab175218 at 1/100 dilution.

Unknown

Immunoprecipitation - Anti-Dlx4/BP1 antibody [EPR9263] - BSA and Azide free (AB249869)

This data was developed using ab175218, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from K562 cell lysate using ab175218 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-Dlx4/BP1 antibody [EPR9263] (<a href='/en-us/products/primary-antibodies/dlx4-bp1-antibody-epr9263-ab175218'>ab175218</a>)

Predicted band size: 26 kDa

false

Supplier Data

Western blot - Anti-Dlx4/BP1 antibody [EPR9263] - BSA and Azide free (AB249869)

This data was developed using ab175218, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Dlx4/BP1 antibody [EPR9263] (<a href='/en-us/products/primary-antibodies/dlx4-bp1-antibody-epr9263-ab175218'>ab175218</a>) at 1/1000 dilution

Lane 1:

SW480 cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

Human placenta tissue lysate at 10 µg

Predicted band size: 26 kDa

false

Unconjugated

Anti-Dlx4/BP1 antibody [EPR9263]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9263

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab249869 is the carrier-free version of ab175218.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May play a role in determining the production of hemoglobin S. May act as a repressor. During embryonic development, plays a role in palatogenesis.

See full target information DLX4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:775642 PubMed35004677

2021

HIF-1α Induces HECTD2 Up-Regulation and Aggravates the Malignant Progression of Renal Cell Cancer Repressing miR-320a.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Lv,Taimin Shen,Juncheng Yao,Qi Yang,Ying Xiang,Zhiwei Ma

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Dlx4/BP1 antibody [EPR9263] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dlx4/BP1 antibody [EPR9263] - BSA and Azide free (AB249869)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dlx4/BP1 antibody [EPR9263] - BSA and Azide free (AB249869)

Immunocytochemistry/ Immunofluorescence - Anti-Dlx4/BP1 antibody [EPR9263] - BSA and Azide free (AB249869)

Immunoprecipitation - Anti-Dlx4/BP1 antibody [EPR9263] - BSA and Azide free (AB249869)

Western blot - Anti-Dlx4/BP1 antibody [EPR9263] - BSA and Azide free (AB249869)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

Product promise