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AB250962

Anti-DMAP1 antibody [EPR18179] - BSA and Azide free

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Rabbit Recombinant Monoclonal DMAP1 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples.

View Alternative Names

KIAA1425, DMAP1, DNA methyltransferase 1-associated protein 1, DNMAP1, DNMT1-associated protein 1

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-DMAP1 antibody [EPR18179] - BSA and Azide free (AB250962)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-DMAP1 antibody [EPR18179] - BSA and Azide free (AB250962)

This data was developed using ab188407, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling DMAP1 with ab188407 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing nuclear staining on HeLa cell line.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows :
1. ab188407 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-DMAP1 antibody [EPR18179] - BSA and Azide free (AB250962)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-DMAP1 antibody [EPR18179] - BSA and Azide free (AB250962)

This data was developed using ab188407, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized F9 (Mouse embryonic carcinoma cell line) cells labeling DMAP1 with ab188407 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing nuclear staining on F9 cell line.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows :
1. ab188407 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

Western blot - Anti-DMAP1 antibody [EPR18179] - BSA and Azide free (AB250962)
  • WB

Supplier Data

Western blot - Anti-DMAP1 antibody [EPR18179] - BSA and Azide free (AB250962)

This data was developed using ab188407, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-DMAP1 antibody [EPR18179] (<a href='/en-us/products/primary-antibodies/dmap1-antibody-epr18179-ab188407'>ab188407</a>) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 2:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 10 µg

Lane 3:

293 (Human epithelial cells from embryonic kidney) whole cell lysate at 10 µg

Lane 4:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 10 µg

Lane 5:

Ramos (Human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

Lane 6:

Neuro-2a (Mouse neuroblastoma cells) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 53 kDa

Observed band size: 53 kDa

false

Exposure time: 3min

Western blot - Anti-DMAP1 antibody [EPR18179] - BSA and Azide free (AB250962)
  • WB

Supplier Data

Western blot - Anti-DMAP1 antibody [EPR18179] - BSA and Azide free (AB250962)

This data was developed using ab188407, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-DMAP1 antibody [EPR18179] (<a href='/en-us/products/primary-antibodies/dmap1-antibody-epr18179-ab188407'>ab188407</a>) at 1/1000 dilution

Lane 1:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 2:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Lane 3:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 53 kDa

Observed band size: 53 kDa

false

Exposure time: 3min

Western blot - Anti-DMAP1 antibody [EPR18179] - BSA and Azide free (AB250962)
  • WB

Lab

Western blot - Anti-DMAP1 antibody [EPR18179] - BSA and Azide free (AB250962)

This data was developed using ab188407, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-DMAP1 antibody [EPR18179] (<a href='/en-us/products/primary-antibodies/dmap1-antibody-epr18179-ab188407'>ab188407</a>) at 1/2000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) cytosolic fraction at 10 µg

Lane 2:

HeLa (Human epithelial cells from cervix adenocarcinoma) nuclear fraction at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 128 kDa,53 kDa

Observed band size: 128 kDa,53 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18179

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab250962 is the carrier-free version of ab188407.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DMAP1 also known as DNA methyltransferase 1-associated protein 1 functions mechanically as a regulatory component in several cellular processes. With an approximate mass of 53 kDa this protein exhibits widespread expression in various tissues reflecting its diverse roles within the cell. The primary function of DMAP1 involves transcriptional regulation through histone modification and chromatin remodeling influencing gene expression patterns.
Biological function summary

DMAP1 participates in gene silencing and regulation through integration into the NuRD complex a critical multi-subunit complex involved in chromatin remodeling and deacetylation of histones. By modulating chromatin structure DMAP1 influences DNA accessibility and transcriptional activation or repression. This protein provides a scaffold for the recruitment of additional factors essential for transcriptional regulatory complexes further enhancing its role in maintaining cellular homeostasis.

Pathways

DMAP1 plays a significant role in the DNA damage response and repair pathways particularly through its interactions with proteins such as DNA methyltransferase 1 (DNMT1) and components of the NuRD complex. Through these interactions DMAP1 aids in the repair of damaged DNA and maintenance of genomic stability. This involvement highlights its connection to cellular processes like cell cycle control and development linking it to broader biological networks.

DMAP1 displays associations with various cancers and neurological disorders reflecting its importance in maintaining proper cellular function. Aberrations in DMAP1 activity or expression have been observed in certain cancers where its interaction with proteins like DNMT1 and components of chromatin remodeling complexes may contribute to tumorigenesis. Similarly its disruption can affect neurodevelopmental processes possibly linking it to neurodegenerative diseases. Understanding DMAP1's role in these conditions can provide insights into therapeutic targets and disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in transcription repression and activation. Its interaction with HDAC2 may provide a mechanism for histone deacetylation in heterochromatin following replication of DNA at late firing origins. Can also repress transcription independently of histone deacetylase activity. May specifically potentiate DAXX-mediated repression of glucocorticoid receptor-dependent transcription. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Participates in the nuclear localization of URI1 and increases its transcriptional corepressor activity.
See full target information DMAP1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com