Rabbit Recombinant Monoclonal DMBT1 antibody. Carrier free. Suitable for WB, IP, IHC-P, Flow Cyt (Intra) and reacts with Recombinant fragment - Human, Human samples.
Images
![Western blot - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (AB316186), expandable thumbnail](https://content.abcam.com/products/images/dmbt1-antibody-epr25722-10-bsa-and-azide-free-ab316186--western-blot-img426398.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (AB316186)")
![Western blot - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (AB316186), expandable thumbnail](https://content.abcam.com/products/images/dmbt1-antibody-epr25722-10-bsa-and-azide-free-ab316186--western-blot-img426396.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (AB316186)")
![Flow Cytometry (Intracellular) - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (AB316186), expandable thumbnail](https://content.abcam.com/products/images/dmbt1-antibody-epr25722-10-bsa-and-azide-free-ab316186--flow-cytometry-intracellular-img426480.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (AB316186)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (AB316186), expandable thumbnail](https://content.abcam.com/products/images/dmbt1-antibody-epr25722-10-bsa-and-azide-free-ab316186--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426452.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (AB316186)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (AB316186), expandable thumbnail](https://content.abcam.com/products/images/dmbt1-antibody-epr25722-10-bsa-and-azide-free-ab316186--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426451.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (AB316186)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IP | IHC-P | Flow Cyt (Intra) | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant fragment - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human, Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Recombinant fragment - Human, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Recombinant fragment - Human, Rat | Dilution info - | Notes - |
Target data
Function
May be considered as a candidate tumor suppressor gene for brain, lung, esophageal, gastric, and colorectal cancers. May play roles in mucosal defense system, cellular immune defense and epithelial differentiation. May play a role as an opsonin receptor for SFTPD and SPAR in macrophage tissues throughout the body, including epithelial cells lining the gastrointestinal tract. May play a role in liver regeneration. May be an important factor in fate decision and differentiation of transit-amplifying ductular (oval) cells within the hepatic lineage. Required for terminal differentiation of columnar epithelial cells during early embryogenesis. May function as a binding protein in saliva for the regulation of taste sensation. Binds to HIV-1 envelope protein and has been shown to both inhibit and facilitate viral transmission. Displays a broad calcium-dependent binding spectrum against both Gram-positive and Gram-negative bacteria, suggesting a role in defense against bacterial pathogens. Binds to a range of poly-sulfated and poly-phosphorylated ligands which may explain its broad bacterial-binding specificity. Inhibits cytoinvasion of S.enterica. Associates with the actin cytoskeleton and is involved in its remodeling during regulated exocytosis. Interacts with pancreatic zymogens in a pH-dependent manner and may act as a Golgi cargo receptor in the regulated secretory pathway of the pancreatic acinar cell.
Alternative names
GP340, DMBT1, Scavenger receptor cysteine-rich domain-containing protein DMBT1, Deleted in malignant brain tumors 1 protein, Glycoprotein 340, Hensin, Salivary agglutinin, Surfactant pulmonary-associated D-binding protein, Gp-340, SAG
Recommended products
Rabbit Recombinant Monoclonal DMBT1 antibody. Carrier free. Suitable for WB, IP, IHC-P, Flow Cyt (Intra) and reacts with Recombinant fragment - Human, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR25722-10
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab316186 is the carrirer-free version of Anti-DMBT1 antibody [EPR25722-10] ab316185.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The DMBT1 protein also known as Deleted in Malignant Brain Tumors 1 plays an important role in the human immune response and tissue homeostasis. It has a molecular mass of approximately 340 kDa. The protein expresses in numerous tissues such as the respiratory tract gastrointestinal tract and reproductive organs suggesting its involvement in various physiological processes. DMBT1 expresses abundantly in epithelial cells where it is also secreted into body fluids.
- Biological function summary
The DMBT1 protein acts as a pattern recognition receptor (PRR) which means it identifies and binds to pathogen-associated molecular patterns. This function helps in the elimination of pathogens and maintaining mucosal immunity. DMBT1 is not typically part of a larger stable protein complex but it interacts with other PRRs to enhance protective responses. It helps balance between immune defense and inflammation indicating its adaptive role in immunity.
- Pathways
The Dmbt1 protein interacts significantly with the immune and inflammatory pathways. It is involved in the Toll-like receptor (TLR) signaling pathway which is important in recognizing microbial components and activating immune responses. Additionally DMBT1 has interactions with the NF-kB pathway which controls inflammatory reactions and immune response. Proteins involved in these pathways related to DMBT1 include TLR4 and MyD88 indicating its role in modulating immune responses.
- Associated diseases and disorders
The Dmbt1 protein is linked to cancer and inflammatory diseases. In certain cancers such as brain and lung cancer it functions as a tumor suppressor. Loss or alteration of the DMBT1 gene can result in reduced immune surveillance leading to tumor progression. It also relates to inflammatory bowel disease where altered DMBT1 expression contributes to intestinal inflammation. Proteins like TLR4 with which DMBT1 interacts are implicated in both cancer and chronic inflammatory responses highlighting their interconnected roles.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Western blot - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (ab316186), expandable thumbnail](https://content.abcam.com/products/images/dmbt1-antibody-epr25722-10-bsa-and-azide-free-ab316186--western-blot-img426398.jpg "Western blot - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (ab316186)")
Western blot - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (ab316186)
This data was developed using Anti-DMBT1 antibody [EPR25722-10] ab316185, the same antibody clone in a different buffer formulation.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-DMBT1 antibody [EPR25722-10] (Anti-DMBT1 antibody [EPR25722-10] ab316185) at 1/1000 dilution
All lanes: His-tagged human DMBT1 recombinant protein fragment at 10 ng with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 60 kDa
Exposure time: 10s
![Western blot - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (ab316186), expandable thumbnail](https://content.abcam.com/products/images/dmbt1-antibody-epr25722-10-bsa-and-azide-free-ab316186--western-blot-img426396.jpg "Western blot - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (ab316186)")
Western blot - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (ab316186)
This data was developed using Anti-DMBT1 antibody [EPR25722-10] ab316185, the same antibody clone in a different buffer formulation.
Nagative control: MCF7(PMID: 32436776)
The molecular weight observed is consistent with what has been described in the literature (PMID:12015815).
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-DMBT1 antibody [EPR25722-10] (Anti-DMBT1 antibody [EPR25722-10] ab316185) at 1/1000 dilution
Lane 1: Capan-1 (human pancreatic adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 270 kDa, 330 kDa, 36 kDa
Exposure time: 70s
![Flow Cytometry (Intracellular) - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (ab316186), expandable thumbnail](https://content.abcam.com/products/images/dmbt1-antibody-epr25722-10-bsa-and-azide-free-ab316186--flow-cytometry-intracellular-img426480.jpg "Flow Cytometry (Intracellular) - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (ab316186)")
Flow Cytometry (Intracellular) - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (ab316186)
This data was developed using Anti-DMBT1 antibody [EPR25722-10] ab316185, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (human breast adenocarcinoma epithelial cell, Left) / Capan-1 (human pancreatic adenocarcinoma epithelial cell, Right) cells labelling DMBT1 with Anti-DMBT1 antibody [EPR25722-10] ab316185 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control.
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Negative control: MCF7.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (ab316186), expandable thumbnail](https://content.abcam.com/products/images/dmbt1-antibody-epr25722-10-bsa-and-azide-free-ab316186--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426452.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (ab316186)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (ab316186)
This data was developed using Anti-DMBT1 antibody [EPR25722-10] ab316185, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling DMBT1 with Anti-DMBT1 antibody [EPR25722-10] ab316185 at 1/2000 (0.242 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining on human liver (PMID: 32436776).
The section was incubated with Anti-DMBT1 antibody [EPR25722-10] ab316185 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (ab316186), expandable thumbnail](https://content.abcam.com/products/images/dmbt1-antibody-epr25722-10-bsa-and-azide-free-ab316186--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426451.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (ab316186)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (ab316186)
This data was developed using Anti-DMBT1 antibody [EPR25722-10] ab316185, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human duodenum tissue labeling DMBT1 with Anti-DMBT1 antibody [EPR25722-10] ab316185 at 1/2000 (0.242 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Cytoplasmic staining of the crypts and very basal parts of human duodenum(PMID: 32436776).
The section was incubated with Anti-DMBT1 antibody [EPR25722-10] ab316185 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (ab316186), expandable thumbnail](https://content.abcam.com/products/images/dmbt1-antibody-epr25722-10-bsa-and-azide-free-ab316186--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426450.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (ab316186)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (ab316186)
This data was developed using Anti-DMBT1 antibody [EPR25722-10] ab316185, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human ileum tissue labeling DMBT1 with Anti-DMBT1 antibody [EPR25722-10] ab316185 at 1/2000 (0.242 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Cytoplasmic staining of the crypts and very basal parts of human ileum (PMID: 32436776).
The section was incubated with Anti-DMBT1 antibody [EPR25722-10] ab316185 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Western blot - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (ab316186), expandable thumbnail](https://content.abcam.com/products/images/dmbt1-antibody-epr25722-10-bsa-and-azide-free-ab316186--western-blot-img426397.jpg "Western blot - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (ab316186)")
Western blot - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (ab316186)
This data was developed using Anti-DMBT1 antibody [EPR25722-10] ab316185, the same antibody clone in a different buffer formulation.
Low expression: U-937, THP-1.
The molecular weight observed is consistent with what has been described in the literature (PMID:12015815).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-DMBT1 antibody [EPR25722-10] (Anti-DMBT1 antibody [EPR25722-10] ab316185) at 1/1000 dilution
Lane 1: SUM149PT (human breast adenocarcinoma cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: U-937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 3: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 270 kDa, 330 kDa, 36 kDa
Exposure time: 70s
![Immunoprecipitation - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (ab316186), expandable thumbnail](https://content.abcam.com/products/images/dmbt1-antibody-epr25722-10-bsa-and-azide-free-ab316186--immunoprecipitation-img426418.jpg "Immunoprecipitation - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (ab316186)")
Immunoprecipitation - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (ab316186)
This data was developed using Anti-DMBT1 antibody [EPR25722-10] ab316185, the same antibody clone in a different buffer formulation.
DMBT1 was immunoprecipitated from 0.35 mg Capan-1 (human pancreas adenocarcinoma epithelial cell) whole cell lysate with Anti-DMBT1 antibody [EPR25722-10] ab316185 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-DMBT1 antibody [EPR25722-10] ab316185 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Capan-1 (human pancreas adenocarcinoma epithelial cell) whole cell lysate
Lane 2: Anti-DMBT1 antibody [EPR25722-10] ab316185 IP in Capan-1 (human pancreas adenocarcinoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-DMBT1 antibody [EPR25722-10] ab316185 in Capan-1 whole cell lysateAll lanes: Immunoprecipitation - Anti-DMBT1 antibody [EPR25722-10] (Anti-DMBT1 antibody [EPR25722-10] ab316185) at 1/30 dilution
All lanes: Capan-1 (human pancreas adenocarcinoma epithelial cell) whole cell lysate with 5% NFDM/TBST
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 41s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (ab316186), expandable thumbnail](https://content.abcam.com/products/images/dmbt1-antibody-epr25722-10-bsa-and-azide-free-ab316186--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426450.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (ab316186)")
![Western blot - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (ab316186), expandable thumbnail](https://content.abcam.com/products/images/dmbt1-antibody-epr25722-10-bsa-and-azide-free-ab316186--western-blot-img426397.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (ab316186)")
![Immunoprecipitation - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (ab316186), expandable thumbnail](https://content.abcam.com/products/images/dmbt1-antibody-epr25722-10-bsa-and-azide-free-ab316186--immunoprecipitation-img426418.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-DMBT1 antibody [EPR25722-10] - BSA and Azide free (ab316186)")
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