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AB313810

Anti-DMBT1 antibody [EPR25722-67] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal DMBT1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

GP340, DMBT1, Scavenger receptor cysteine-rich domain-containing protein DMBT1, Deleted in malignant brain tumors 1 protein, Glycoprotein 340, Hensin, Salivary agglutinin, Surfactant pulmonary-associated D-binding protein, Gp-340, SAG

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Sandwich ELISA - Anti-DMBT1 antibody [EPR25722-67] - BSA and Azide free (Capture) (AB313810)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-DMBT1 antibody [EPR25722-67] - BSA and Azide free (Capture) (AB313810)

Sandwich ELISA of ab313809 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25722-67

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DMBT1 protein also known as Deleted in Malignant Brain Tumors 1 plays an important role in the human immune response and tissue homeostasis. It has a molecular mass of approximately 340 kDa. The protein expresses in numerous tissues such as the respiratory tract gastrointestinal tract and reproductive organs suggesting its involvement in various physiological processes. DMBT1 expresses abundantly in epithelial cells where it is also secreted into body fluids.
Biological function summary

The DMBT1 protein acts as a pattern recognition receptor (PRR) which means it identifies and binds to pathogen-associated molecular patterns. This function helps in the elimination of pathogens and maintaining mucosal immunity. DMBT1 is not typically part of a larger stable protein complex but it interacts with other PRRs to enhance protective responses. It helps balance between immune defense and inflammation indicating its adaptive role in immunity.

Pathways

The Dmbt1 protein interacts significantly with the immune and inflammatory pathways. It is involved in the Toll-like receptor (TLR) signaling pathway which is important in recognizing microbial components and activating immune responses. Additionally DMBT1 has interactions with the NF-kB pathway which controls inflammatory reactions and immune response. Proteins involved in these pathways related to DMBT1 include TLR4 and MyD88 indicating its role in modulating immune responses.

The Dmbt1 protein is linked to cancer and inflammatory diseases. In certain cancers such as brain and lung cancer it functions as a tumor suppressor. Loss or alteration of the DMBT1 gene can result in reduced immune surveillance leading to tumor progression. It also relates to inflammatory bowel disease where altered DMBT1 expression contributes to intestinal inflammation. Proteins like TLR4 with which DMBT1 interacts are implicated in both cancer and chronic inflammatory responses highlighting their interconnected roles.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be considered as a candidate tumor suppressor gene for brain, lung, esophageal, gastric, and colorectal cancers. May play roles in mucosal defense system, cellular immune defense and epithelial differentiation. May play a role as an opsonin receptor for SFTPD and SPAR in macrophage tissues throughout the body, including epithelial cells lining the gastrointestinal tract. May play a role in liver regeneration. May be an important factor in fate decision and differentiation of transit-amplifying ductular (oval) cells within the hepatic lineage. Required for terminal differentiation of columnar epithelial cells during early embryogenesis. May function as a binding protein in saliva for the regulation of taste sensation. Binds to HIV-1 envelope protein and has been shown to both inhibit and facilitate viral transmission. Displays a broad calcium-dependent binding spectrum against both Gram-positive and Gram-negative bacteria, suggesting a role in defense against bacterial pathogens. Binds to a range of poly-sulfated and poly-phosphorylated ligands which may explain its broad bacterial-binding specificity. Inhibits cytoinvasion of S.enterica. Associates with the actin cytoskeleton and is involved in its remodeling during regulated exocytosis. Interacts with pancreatic zymogens in a pH-dependent manner and may act as a Golgi cargo receptor in the regulated secretory pathway of the pancreatic acinar cell.
See full target information DMBT1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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