Mouse Monoclonal DMC1 antibody. Suitable for IP, Flow Cyt, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human, Cow samples. Cited in 25 publications. Immunogen corresponding to Recombinant Protein within Human DMC1.
Images
![Flow Cytometry - Anti-DMC1 antibody [2H12/4] (AB11054), expandable thumbnail](https://content.abcam.com/products/images/dmc1-antibody-2h12-4-ab11054--flow-cytometry-img63333.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-DMC1 antibody [2H12/4] (AB11054)")
Publications
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- Zoological research 43:537-5512022Production of functional sperm from -cultured premeiotic spermatogonia in a marine fish.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHong Zhang,Wan-Wan Zhang,Cheng-Yu Mo,Meng-Dan Dong,Kun-Tong Jia,Wei Liu,Mei-Sheng YiPubMed 35616259
- Nature communications 12:46052021BRCA2 binding through a cryptic repeated motif to HSF2BP oligomers does not impact meiotic recombination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRania Ghouil,Simona Miron,Lieke Koornneef,Jasper Veerman,Maarten W Paul,Marie-Hélène Le Du,Esther Sleddens-Linkels,Sari E van Rossum-Fikkert,Yvette van Loon,Natalia Felipe-Medina,Alberto M Pendas,Alex Maas,Jeroen Essers,Pierre Legrand,Willy M Baarends,Roland Kanaar,Sophie Zinn-Justin,Alex N ZelenskyPubMed 34326328
- Nature communications 12:31762021The SUN1-SPDYA interaction plays an essential role in meiosis prophase I.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanyan Chen,Yan Wang,Juan Chen,Wu Zuo,Yong Fan,Sijia Huang,Yongmei Liu,Guangming Chen,Qing Li,Jinsong Li,Jian Wu,Qian Bian,Chenhui Huang,Ming LeiPubMed 34039995
- Theranostics 11:5010-50272021Deciphering the autophagy regulatory network via single-cell transcriptome analysis reveals a requirement for autophagy homeostasis in spermatogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMei Wang,Yanwen Xu,Yuncong Zhang,Yuhan Chen,Gang Chang,Geng An,Xinyan Yang,Caihong Zheng,Jiexiang Zhao,Zhaoting Liu,Dazhuang Wang,Kai Miao,Shuan Rao,Meng Dai,Dong Wang,Xiao-Yang ZhaoPubMed 33754041
- Molecular cell 79:689-701.e102020The Configuration of RPA, RAD51, and DMC1 Binding in Meiosis Reveals the Nature of Critical Recombination Intermediates.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnjali Gupta Hinch et. al.PubMed 32610038
- PLoS genetics 16:e10085952020Super-resolution imaging of RAD51 and DMC1 in DNA repair foci reveals dynamic distribution patterns in meiotic prophase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohan A Slotman et. al.PubMed 32502153
- International journal of molecular sciences 21:2020"Mitotic Slippage" and Extranuclear DNA in Cancer Chemoresistance: A Focus on Telomeres.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKristine Salmina et. al.PubMed 32316332
- Journal of clinical medicine 9:2020Exogenous Gonadotrophin Stimulation Induces Partial Maturation of Human Sertoli Cells in a Testicular Xenotransplantation Model for Fertility Preservation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarsida Hutka,Lee B Smith,Ellen Goossens,W Hamish B Wallace,Jan-Bernd Stukenborg,Rod T MitchellPubMed 31963729
- Nutrients 12:2020The Preparation of Ginsenoside Rg5, Its Antitumor Activity against Breast Cancer Cells and Its Targeting of PI3K.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYannan Liu et. al.PubMed 31963684
- Molecular human reproduction 25:668-6732019A predicted deleterious allele of the essential meiosis gene MND1, present in ~ 3% of East Asians, does not disrupt reproduction in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTina N Tran,Julianna Martinez,John C SchimentiPubMed 31393579
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 99.98% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Protein within Human DMC1. The exact immunogen used to generate this antibody is proprietary information. Database link Q14565
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Expected | Tested | Expected | Expected | Expected |
| Mouse | Expected | Predicted | Expected | Expected | Expected |
| Rat | Expected | Predicted | Expected | Expected | Expected |
| Cow | Expected | Predicted | Expected | Expected | Expected |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg for 106 Cells | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Rat, Mouse | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Fix cells using a 4% paraformaldehyde solution and permeabilize using SDS (0.03% in a 4% paraformaldehyde solution). Incubate antibody overnight. |
Species Rat | Dilution info - | Notes Fix cells using a 4% paraformaldehyde solution and permeabilize using SDS (0.03% in a 4% paraformaldehyde solution). Incubate antibody overnight. |
Species Human | Dilution info - | Notes Fix cells using a 4% paraformaldehyde solution and permeabilize using SDS (0.03% in a 4% paraformaldehyde solution). Incubate antibody overnight. |
Species Cow | Dilution info - | Notes Fix cells using a 4% paraformaldehyde solution and permeabilize using SDS (0.03% in a 4% paraformaldehyde solution). Incubate antibody overnight. |
Associated Products
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Target data
Function
Participates in meiotic recombination, specifically in homologous strand assimilation, which is required for the resolution of meiotic double-strand breaks.
Alternative names
DMC1H, LIM15, DMC1, Meiotic recombination protein DMC1/LIM15 homolog
Recommended products
Mouse Monoclonal DMC1 antibody. Suitable for IP, Flow Cyt, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human, Cow samples. Cited in 25 publications. Immunogen corresponding to Recombinant Protein within Human DMC1.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 99.98% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Protein within Human DMC1. The exact immunogen used to generate this antibody is proprietary information. Database link Q14565
- Clone number
- 2H12/4
- Purification technique
- Affinity purification Protein A
- Specificity
Specific for DMC1 - does not cross-react with the related protein Rad51. The antibody was tested on mouse meiotic chromosomes. It allowed localisation of the Dmc1 protein as numerous foci in the Leptotene and Zygotene stages of meiotic prophase, and fewer foci in the early Pachytene stage of meiotic prophase.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DMC1 also known as DNA Meiotic Recombinase 1 is an important protein involved in homologous recombination during meiosis. It plays a mechanical role by facilitating the exchange of genetic material between parental chromosomes. DMC1 is a 37-kilodalton protein and belongs to the recombinase family. The protein is mainly expressed in germ cells of both males and females particularly in those undergoing meiosis where it controls the DNA strand exchange process important for genetic diversity.
- Biological function summary
DMC1 ensures accurate and efficient repair of double-strand breaks during meiosis a critical function for chromosome segregation and gamete viability. It works in concert with other proteins like RAD51 to form a nucleoprotein filament on the single-stranded DNA that promotes homologous pairing and strand exchange. This coordinated action is essential for forming synaptonemal complexes which stabilize homologous chromosomes during prophase I of meiosis.
- Pathways
DMC1's role is integral to the homologous recombination repair pathway. It interplays specifically with RAD51 and other recombinases to regulate genetic recombination events. The protein contributes to DNA damage response pathways and is critical for the maintenance and integrity of genetic information during cell division.
- Associated diseases and disorders
Mutations or regulatory dysfunctions in DMC1 have been associated with infertility particularly in disorders affecting spermatogenesis and oogenesis. The protein’s malfunction can disrupt meiotic progression leading to aneuploidies and miscarriages. Additionally DMC1 mutations may connect to cancer pathways as faulty homologous recombination increases genomic instability often observed in various types of cancers highlighting potential therapeutic interactions with proteins like BRCA1 and BRCA2.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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1 product image
![Flow Cytometry - Anti-DMC1 antibody [2H12/4] (ab11054), expandable thumbnail](https://content.abcam.com/products/images/dmc1-antibody-2h12-4-ab11054--flow-cytometry-img63333.jpg "Flow Cytometry - Anti-DMC1 antibody [2H12/4] (ab11054)")
Flow Cytometry - Anti-DMC1 antibody [2H12/4] (ab11054)
Overlay histogram showing HeLa cells stained with ab11054 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11054, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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