AB11054

Anti-DMC1 antibody [2H12/4]

Name: Anti-DMC1 antibody [2H12/4] (ab11054) | Abcam
Brand: Abcam Limited
SKU: AB11054
Price: 510 USD
Availability: InStock
Rating: 3 (3 reviews)

(3 Reviews)

(29 Publications)

Mouse Monoclonal DMC1 antibody. Suitable for IP, Flow Cyt, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human, Cow samples. Cited in 29 publications. Immunogen corresponding to Recombinant Protein within Human DMC1.

View Alternative Names

DMC1H, LIM15, DMC1, Meiotic recombination protein DMC1/LIM15 homolog

1 Images

Flow Cytometry - Anti-DMC1 antibody [2H12/4] (AB11054)

Flow Cyt

Unknown

Flow Cytometry - Anti-DMC1 antibody [2H12/4] (AB11054)

Overlay histogram showing HeLa cells stained with ab11054 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11054, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2H12/4

Isotype

IgG2a

Carrier free

Reacts with

Human, Cow, Rat, Mouse

Applications

IP, Flow Cyt, IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Protein within Human DMC1. The exact immunogen used to generate this antibody is proprietary information.

Q14565

Specificity

Specific for DMC1 - does not cross-react with the related protein Rad51. The antibody was tested on mouse meiotic chromosomes. It allowed localisation of the Dmc1 protein as numerous foci in the Leptotene and Zygotene stages of meiotic prophase, and fewer foci in the early Pachytene stage of meiotic prophase.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "Fix cells using a 4% paraformaldehyde solution and permeabilize using SDS (0.03% in a 4% paraformaldehyde solution). Incubate antibody overnight." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "Fix cells using a 4% paraformaldehyde solution and permeabilize using SDS (0.03% in a 4% paraformaldehyde solution). Incubate antibody overnight." }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "Fix cells using a 4% paraformaldehyde solution and permeabilize using SDS (0.03% in a 4% paraformaldehyde solution). Incubate antibody overnight." }, "Cow": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "Fix cells using a 4% paraformaldehyde solution and permeabilize using SDS (0.03% in a 4% paraformaldehyde solution). Incubate antibody overnight." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DMC1 also known as DNA Meiotic Recombinase 1 is an important protein involved in homologous recombination during meiosis. It plays a mechanical role by facilitating the exchange of genetic material between parental chromosomes. DMC1 is a 37-kilodalton protein and belongs to the recombinase family. The protein is mainly expressed in germ cells of both males and females particularly in those undergoing meiosis where it controls the DNA strand exchange process important for genetic diversity.

Biological function summary

DMC1 ensures accurate and efficient repair of double-strand breaks during meiosis a critical function for chromosome segregation and gamete viability. It works in concert with other proteins like RAD51 to form a nucleoprotein filament on the single-stranded DNA that promotes homologous pairing and strand exchange. This coordinated action is essential for forming synaptonemal complexes which stabilize homologous chromosomes during prophase I of meiosis.

Pathways

DMC1's role is integral to the homologous recombination repair pathway. It interplays specifically with RAD51 and other recombinases to regulate genetic recombination events. The protein contributes to DNA damage response pathways and is critical for the maintenance and integrity of genetic information during cell division.

Mutations or regulatory dysfunctions in DMC1 have been associated with infertility particularly in disorders affecting spermatogenesis and oogenesis. The protein’s malfunction can disrupt meiotic progression leading to aneuploidies and miscarriages. Additionally DMC1 mutations may connect to cancer pathways as faulty homologous recombination increases genomic instability often observed in various types of cancers highlighting potential therapeutic interactions with proteins like BRCA1 and BRCA2.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Participates in meiotic recombination, specifically in homologous strand assimilation, which is required for the resolution of meiotic double-strand breaks.

See full target information DMC1

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Publications (29)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:3613 PubMed40240347

2025

Mouse MRE11-RAD50-NBS1 is needed to start and extend meiotic DNA end resection.

Applications

Unspecified application

Species

Unspecified reactive species

Soonjoung Kim,Shintaro Yamada,Tao Li,Claudia Canasto-Chibuque,Jun Hyun Kim,Marina Marcet-Ortega,Jiaqi Xu,Diana Y Eng,Laura Feeney,John H J Petrini,Scott Keeney

Bio-protocol 13:e4780 PubMed37497444

2023

Three-color dSTORM Imaging and Analysis of Recombination Foci in Mouse Spread Meiotic Nuclei.

Applications

Unspecified application

Species

Unspecified reactive species

Lieke Koornneef,Maarten W Paul,Adriaan B Houtsmuller,Willy M Baarends,Johan A Slotman

PLoS genetics 18:e1010046 PubMed35857787

2022

Multi-color dSTORM microscopy in Hormad1-/- spermatocytes reveals alterations in meiotic recombination intermediates and synaptonemal complex structure.

Applications

Unspecified application

Species

Unspecified reactive species

Lieke Koornneef,Johan A Slotman,Esther Sleddens-Linkels,Wiggert A van Cappellen,Marco Barchi,Attila Tóth,Joost Gribnau,Adriaan B Houtsmuller,Willy M Baarends

Zoological research 43:537-551 PubMed35616259

2022

Production of functional sperm from -cultured premeiotic spermatogonia in a marine fish.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Zhang,Wan-Wan Zhang,Cheng-Yu Mo,Meng-Dan Dong,Kun-Tong Jia,Wei Liu,Mei-Sheng Yi

Nature communications 12:4605 PubMed34326328

2021

BRCA2 binding through a cryptic repeated motif to HSF2BP oligomers does not impact meiotic recombination.

Applications

Unspecified application

Species

Unspecified reactive species

Rania Ghouil,Simona Miron,Lieke Koornneef,Jasper Veerman,Maarten W Paul,Marie-Hélène Le Du,Esther Sleddens-Linkels,Sari E van Rossum-Fikkert,Yvette van Loon,Natalia Felipe-Medina,Alberto M Pendas,Alex Maas,Jeroen Essers,Pierre Legrand,Willy M Baarends,Roland Kanaar,Sophie Zinn-Justin,Alex N Zelensky

Nature communications 12:3176 PubMed34039995

2021

The SUN1-SPDYA interaction plays an essential role in meiosis prophase I.

Applications

Unspecified application

Species

Unspecified reactive species

Yanyan Chen,Yan Wang,Juan Chen,Wu Zuo,Yong Fan,Sijia Huang,Yongmei Liu,Guangming Chen,Qing Li,Jinsong Li,Jian Wu,Qian Bian,Chenhui Huang,Ming Lei

Theranostics 11:5010-5027 PubMed33754041

2021

Deciphering the autophagy regulatory network via single-cell transcriptome analysis reveals a requirement for autophagy homeostasis in spermatogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Wang,Yanwen Xu,Yuncong Zhang,Yuhan Chen,Gang Chang,Geng An,Xinyan Yang,Caihong Zheng,Jiexiang Zhao,Zhaoting Liu,Dazhuang Wang,Kai Miao,Shuan Rao,Meng Dai,Dong Wang,Xiao-Yang Zhao

Molecular cell 79:689-701.e10 PubMed32610038

2020

The Configuration of RPA, RAD51, and DMC1 Binding in Meiosis Reveals the Nature of Critical Recombination Intermediates.

Applications

Unspecified application

Species

Unspecified reactive species

Anjali Gupta Hinch,Philipp W Becker,Tao Li,Daniela Moralli,Gang Zhang,Clare Bycroft,Catherine Green,Scott Keeney,Qinghua Shi,Benjamin Davies,Peter Donnelly

PLoS genetics 16:e1008595 PubMed32502153

2020

Super-resolution imaging of RAD51 and DMC1 in DNA repair foci reveals dynamic distribution patterns in meiotic prophase.

Applications

Unspecified application

Species

Unspecified reactive species

Johan A Slotman,Maarten W Paul,Fabrizia Carofiglio,H Martijn de Gruiter,Tessa Vergroesen,Lieke Koornneef,Wiggert A van Cappellen,Adriaan B Houtsmuller,Willy M Baarends

International journal of molecular sciences 21: PubMed32316332

2020

"Mitotic Slippage" and Extranuclear DNA in Cancer Chemoresistance: A Focus on Telomeres.

Applications

Unspecified application

Species

Unspecified reactive species

Kristine Salmina,Agnieszka Bojko,Inna Inashkina,Karolina Staniak,Magdalena Dudkowska,Petar Podlesniy,Felikss Rumnieks,Ninel M Vainshelbaum,Dace Pjanova,Ewa Sikora,Jekaterina Erenpreisa

View all publications

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