JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB237733

Anti-DMC1 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal DMC1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DMC1 aa 1-200.

View Alternative Names

DMC1H, LIM15, DMC1, Meiotic recombination protein DMC1/LIM15 homolog

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody (AB237733)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody (AB237733)

Paraffin-embedded human testis tissue stained for DMC1 using ab237733 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-DMC1 antibody (AB237733)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DMC1 antibody (AB237733)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for DMC1 (green) using ab237733 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Western blot - Anti-DMC1 antibody (AB237733)
  • WB

Supplier Data

Western blot - Anti-DMC1 antibody (AB237733)

All lanes:

Western blot - Anti-DMC1 antibody (ab237733) at 1/500 dilution

Lane 1:

Raji (human Burkitt's lymphoma cell line) whole cell lysate

Lane 2:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human DMC1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q14565

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DMC1 also known as DNA Meiotic Recombinase 1 is an important protein involved in homologous recombination during meiosis. It plays a mechanical role by facilitating the exchange of genetic material between parental chromosomes. DMC1 is a 37-kilodalton protein and belongs to the recombinase family. The protein is mainly expressed in germ cells of both males and females particularly in those undergoing meiosis where it controls the DNA strand exchange process important for genetic diversity.
Biological function summary

DMC1 ensures accurate and efficient repair of double-strand breaks during meiosis a critical function for chromosome segregation and gamete viability. It works in concert with other proteins like RAD51 to form a nucleoprotein filament on the single-stranded DNA that promotes homologous pairing and strand exchange. This coordinated action is essential for forming synaptonemal complexes which stabilize homologous chromosomes during prophase I of meiosis.

Pathways

DMC1's role is integral to the homologous recombination repair pathway. It interplays specifically with RAD51 and other recombinases to regulate genetic recombination events. The protein contributes to DNA damage response pathways and is critical for the maintenance and integrity of genetic information during cell division.

Mutations or regulatory dysfunctions in DMC1 have been associated with infertility particularly in disorders affecting spermatogenesis and oogenesis. The protein's malfunction can disrupt meiotic progression leading to aneuploidies and miscarriages. Additionally DMC1 mutations may connect to cancer pathways as faulty homologous recombination increases genomic instability often observed in various types of cancers highlighting potential therapeutic interactions with proteins like BRCA1 and BRCA2.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Participates in meiotic recombination, specifically in homologous strand assimilation, which is required for the resolution of meiotic double-strand breaks.
See full target information DMC1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 25: PubMed35485284

2022

TXNDC9 knockdown inhibits lung adenocarcinoma progression by targeting YWHAG.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wang,Xiaotao Pan,Jie Li,Jin Zhao
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB97313

Anti-DMC1 antibody

Western blot - Anti-DMC1 antibody (AB97313)

  • 1
  • 1
Primary Antibodies

AB96613

Anti-DMC1 antibody

Western blot - Anti-DMC1 antibody (AB96613)

  • 1
  • 1
Primary Antibodies

AB11054

Anti-DMC1 antibody [2H12/4]

Flow Cytometry - Anti-DMC1 antibody [2H12/4] (AB11054)

  • 3
  • 3
Primary Antibodies

AB245217

Anti-DMC1 antibody [EPR23207-130]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] (AB245217)

  • 5
  • 1
Primary Antibodies

AB269869

Anti-DMC1 antibody [EPR23207-130] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] - BSA and Azide free (AB269869)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB97672

Anti-SCP3 antibody [Cor 10G11/7]

BOND RX™ Validated|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (AB97672)

  • 5
  • 10
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB81299

Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (AB81299)

  • 4
  • 17
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com