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Rabbit Recombinant Monoclonal DMC1 antibody. Suitable for IP, WB, IHC-P, IHC-Fr and reacts with Mouse, Rat samples.


Images

Western blot - Anti-DMC1 antibody [EPR23207-130] (AB245217), expandable thumbnail
  • Western blot - Anti-DMC1 antibody [EPR23207-130] (AB245217), expandable thumbnail
  • Immunoprecipitation - Anti-DMC1 antibody [EPR23207-130] (AB245217), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-DMC1 antibody [EPR23207-130] (AB245217), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-DMC1 antibody [EPR23207-130] (AB245217), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PIHC-Fr
Human
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Tested
Rat
Expected
Tested
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/4000
Notes

-

Species
Rat
Dilution info
1/4000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

-

Species
Rat
Dilution info
1/100
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Participates in meiotic recombination, specifically in homologous strand assimilation, which is required for the resolution of meiotic double-strand breaks.

Alternative names

Dmc1h, Lim15, Dmc1, Meiotic recombination protein DMC1/LIM15 homolog

Recommended products

Rabbit Recombinant Monoclonal DMC1 antibody. Suitable for IP, WB, IHC-P, IHC-Fr and reacts with Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR23207-130
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

DMC1 also known as DNA Meiotic Recombinase 1 is an important protein involved in homologous recombination during meiosis. It plays a mechanical role by facilitating the exchange of genetic material between parental chromosomes. DMC1 is a 37-kilodalton protein and belongs to the recombinase family. The protein is mainly expressed in germ cells of both males and females particularly in those undergoing meiosis where it controls the DNA strand exchange process important for genetic diversity.

Biological function summary

DMC1 ensures accurate and efficient repair of double-strand breaks during meiosis a critical function for chromosome segregation and gamete viability. It works in concert with other proteins like RAD51 to form a nucleoprotein filament on the single-stranded DNA that promotes homologous pairing and strand exchange. This coordinated action is essential for forming synaptonemal complexes which stabilize homologous chromosomes during prophase I of meiosis.

Pathways

DMC1's role is integral to the homologous recombination repair pathway. It interplays specifically with RAD51 and other recombinases to regulate genetic recombination events. The protein contributes to DNA damage response pathways and is critical for the maintenance and integrity of genetic information during cell division.

Associated diseases and disorders

Mutations or regulatory dysfunctions in DMC1 have been associated with infertility particularly in disorders affecting spermatogenesis and oogenesis. The protein’s malfunction can disrupt meiotic progression leading to aneuploidies and miscarriages. Additionally DMC1 mutations may connect to cancer pathways as faulty homologous recombination increases genomic instability often observed in various types of cancers highlighting potential therapeutic interactions with proteins like BRCA1 and BRCA2.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-DMC1 antibody [EPR23207-130] (ab245217), expandable thumbnail

    Western blot - Anti-DMC1 antibody [EPR23207-130] (ab245217)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 22156371).

    Negative control: Spleen (PMID: 21085687).

    Exposure time: 26 seconds

    All lanes: Western blot - Anti-DMC1 antibody [EPR23207-130] (ab245217) at 1/1000 dilution

    Lane 1: Mouse testis lysate at 20 µg

    Lane 2: Mouse spleen lysate at 20 µg

    Lane 3: Rat testis lysate at 20 µg

    Lane 4: Rat spleen lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 38 kDa

    Observed band size: 38 kDa

  • Western blot - Anti-DMC1 antibody [EPR23207-130] (ab245217), expandable thumbnail

    Western blot - Anti-DMC1 antibody [EPR23207-130] (ab245217)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    No cross-reactivity with mouse Rad51 is observed.

    Exposure time: Lane 3 seconds; Lane 2:81 seconds.

    All lanes: Western blot - Anti-DMC1 antibody [EPR23207-130] (ab245217) at 1/1000 dilution

    Lane 1: His-tagged mouse DMC1 recombinant protein (aa2-340) at 10 µg

    Lane 2: His-tagged mouse Rad51 recombinant protein (aa2-339) at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 38 kDa

    Observed band size: 38 kDa

  • Immunoprecipitation - Anti-DMC1 antibody [EPR23207-130] (ab245217), expandable thumbnail

    Immunoprecipitation - Anti-DMC1 antibody [EPR23207-130] (ab245217)

    DMC1 was immunoprecipitated from 0.35 mg Mouse testis lysate 10ug with ab245217 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab245217 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution. Lane 1: Mouse testis lysate 10ug

    Lane 2: ab245217 IP in Mouse testis lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab245217 in Mouse testis lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds

    All lanes: Immunoprecipitation - Anti-DMC1 antibody [EPR23207-130] (ab245217)

    Predicted band size: 38 kDa, 87 kDa

  • Immunohistochemistry (Frozen sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Rat testis tissue labeling VSIG4 with ab245217 at 1/100 (6.7 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

  • Immunohistochemistry (Frozen sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Mouse testis tissue labeling VSIG4 with ab245217 at 1/100 (6.7 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling DMC1 with ab245217 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: No staining on mouse cerebrum. The section was incubated with ab245217 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling DMC1 with ab245217 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on spermatocytes of rat testis. The section was incubated with ab245217 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling DMC1 with ab245217 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on spermatocytes of mouse testis. The section was incubated with ab245217 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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