Rabbit Recombinant Monoclonal DMC1 antibody. Carrier free. Suitable for IP, WB, IHC-P, IHC-Fr and reacts with Mouse, Rat samples.
Images
![Immunohistochemistry (Frozen sections) - Anti-DMC1 antibody [EPR23207-130] - BSA and Azide free (AB269869), expandable thumbnail](https://content.abcam.com/products/images/dmc1-antibody-epr23207-130-bsa-and-azide-free-ab269869--immunohistochemistry-frozen-sections-img14351.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-DMC1 antibody [EPR23207-130] - BSA and Azide free (AB269869)")
![Immunohistochemistry (Frozen sections) - Anti-DMC1 antibody [EPR23207-130] - BSA and Azide free (AB269869), expandable thumbnail](https://content.abcam.com/products/images/dmc1-antibody-epr23207-130-bsa-and-azide-free-ab269869--immunohistochemistry-frozen-sections-img14364.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-DMC1 antibody [EPR23207-130] - BSA and Azide free (AB269869)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] - BSA and Azide free (AB269869), expandable thumbnail](https://content.abcam.com/products/images/dmc1-antibody-epr23207-130-bsa-and-azide-free-ab269869--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118330.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] - BSA and Azide free (AB269869)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] - BSA and Azide free (AB269869), expandable thumbnail](https://content.abcam.com/products/images/dmc1-antibody-epr23207-130-bsa-and-azide-free-ab269869--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img73079.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] - BSA and Azide free (AB269869)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] - BSA and Azide free (AB269869), expandable thumbnail](https://content.abcam.com/products/images/dmc1-antibody-epr23207-130-bsa-and-azide-free-ab269869--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img22291.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] - BSA and Azide free (AB269869)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | IHC-Fr | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Expected | Expected | Tested | Tested |
| Rat | Expected | Expected | Tested | Tested |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Participates in meiotic recombination, specifically in homologous strand assimilation, which is required for the resolution of meiotic double-strand breaks.
Alternative names
Dmc1h, Lim15, Dmc1, Meiotic recombination protein DMC1/LIM15 homolog
Recommended products
Rabbit Recombinant Monoclonal DMC1 antibody. Carrier free. Suitable for IP, WB, IHC-P, IHC-Fr and reacts with Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23207-130
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab269869 is the carrier-free version of Anti-DMC1 antibody [EPR23207-130] ab245217.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DMC1 also known as DNA Meiotic Recombinase 1 is an important protein involved in homologous recombination during meiosis. It plays a mechanical role by facilitating the exchange of genetic material between parental chromosomes. DMC1 is a 37-kilodalton protein and belongs to the recombinase family. The protein is mainly expressed in germ cells of both males and females particularly in those undergoing meiosis where it controls the DNA strand exchange process important for genetic diversity.
- Biological function summary
DMC1 ensures accurate and efficient repair of double-strand breaks during meiosis a critical function for chromosome segregation and gamete viability. It works in concert with other proteins like RAD51 to form a nucleoprotein filament on the single-stranded DNA that promotes homologous pairing and strand exchange. This coordinated action is essential for forming synaptonemal complexes which stabilize homologous chromosomes during prophase I of meiosis.
- Pathways
DMC1's role is integral to the homologous recombination repair pathway. It interplays specifically with RAD51 and other recombinases to regulate genetic recombination events. The protein contributes to DNA damage response pathways and is critical for the maintenance and integrity of genetic information during cell division.
- Associated diseases and disorders
Mutations or regulatory dysfunctions in DMC1 have been associated with infertility particularly in disorders affecting spermatogenesis and oogenesis. The protein’s malfunction can disrupt meiotic progression leading to aneuploidies and miscarriages. Additionally DMC1 mutations may connect to cancer pathways as faulty homologous recombination increases genomic instability often observed in various types of cancers highlighting potential therapeutic interactions with proteins like BRCA1 and BRCA2.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Frozen sections) - Anti-DMC1 antibody [EPR23207-130] - BSA and Azide free (ab269869), expandable thumbnail](https://content.abcam.com/products/images/dmc1-antibody-epr23207-130-bsa-and-azide-free-ab269869--immunohistochemistry-frozen-sections-img14351.jpg "Immunohistochemistry (Frozen sections) - Anti-DMC1 antibody [EPR23207-130] - BSA and Azide free (ab269869)")
Immunohistochemistry (Frozen sections) - Anti-DMC1 antibody [EPR23207-130] - BSA and Azide free (ab269869)
Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Rat testis tissue labeling VSIG4 with Anti-DMC1 antibody [EPR23207-130] ab245217 at 1/100 (6.7 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DMC1 antibody [EPR23207-130] ab245217).
![Immunohistochemistry (Frozen sections) - Anti-DMC1 antibody [EPR23207-130] - BSA and Azide free (ab269869), expandable thumbnail](https://content.abcam.com/products/images/dmc1-antibody-epr23207-130-bsa-and-azide-free-ab269869--immunohistochemistry-frozen-sections-img14364.jpg "Immunohistochemistry (Frozen sections) - Anti-DMC1 antibody [EPR23207-130] - BSA and Azide free (ab269869)")
Immunohistochemistry (Frozen sections) - Anti-DMC1 antibody [EPR23207-130] - BSA and Azide free (ab269869)
Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Mouse testis tissue labeling VSIG4 with Anti-DMC1 antibody [EPR23207-130] ab245217 at 1/100 (6.7 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DMC1 antibody [EPR23207-130] ab245217).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] - BSA and Azide free (ab269869), expandable thumbnail](https://content.abcam.com/products/images/dmc1-antibody-epr23207-130-bsa-and-azide-free-ab269869--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118330.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] - BSA and Azide free (ab269869)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] - BSA and Azide free (ab269869)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling DMC1 with Anti-DMC1 antibody [EPR23207-130] ab245217 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: No staining on mouse cerebrum. The section was incubated with Anti-DMC1 antibody [EPR23207-130] ab245217 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DMC1 antibody [EPR23207-130] ab245217).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] - BSA and Azide free (ab269869), expandable thumbnail](https://content.abcam.com/products/images/dmc1-antibody-epr23207-130-bsa-and-azide-free-ab269869--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img73079.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] - BSA and Azide free (ab269869)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] - BSA and Azide free (ab269869)
Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling DMC1 with Anti-DMC1 antibody [EPR23207-130] ab245217 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on spermatocytes of rat testis. The section was incubated with Anti-DMC1 antibody [EPR23207-130] ab245217 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DMC1 antibody [EPR23207-130] ab245217).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] - BSA and Azide free (ab269869), expandable thumbnail](https://content.abcam.com/products/images/dmc1-antibody-epr23207-130-bsa-and-azide-free-ab269869--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img22291.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] - BSA and Azide free (ab269869)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] - BSA and Azide free (ab269869)
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling DMC1 with Anti-DMC1 antibody [EPR23207-130] ab245217 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on spermatocytes of mouse testis. The section was incubated with Anti-DMC1 antibody [EPR23207-130] ab245217 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DMC1 antibody [EPR23207-130] ab245217).
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