AB198292

Anti-DMGDH antibody [EPR16908]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal DMGDH antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ME2GLYDH, DMGDH

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMGDH antibody [EPR16908] (AB198292)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMGDH antibody [EPR16908] (AB198292)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling DMGDH with ab198292 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin.

Inset image : negative control obtained using PBS instead of ab198292 and secondary antibody only.
Note : Cytoplasm staining on human liver tissue was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMGDH antibody [EPR16908] (AB198292)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DMGDH with ab198292 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin.

Inset image : negative control obtained using PBS instead of ab198292 and secondary antibody only.
Note : Cytoplasm staining on human kidney tissue was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Unknown

Immunoprecipitation - Anti-DMGDH antibody [EPR16908] (AB198292)

DMGDH protein was immunoprecipitation from 1mg of Human fetal liver whole cell lysate with ab198292 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab198292 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1000 dilution. Lane 1 : Input, Human fetal liver whole cell lysate, 10ug. Lane 2 : IP of DMGDH from Human fetal liver whole cell lysate. Lane 3 : IP using Rabbit monoclonal IgG (ab172730) instead of ab198292 in Human fetal liver whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-DMGDH antibody [EPR16908] (ab198292)

Predicted band size: 97 kDa

Observed band size: 97 kDa

false

Exposure time: 10s

Supplier Data

Western blot - Anti-DMGDH antibody [EPR16908] (AB198292)

5% NFDM/TBST : Blocking and diluting buffer.

All lanes:

Western blot - Anti-DMGDH antibody [EPR16908] (ab198292) at 1/1000 dilution

All lanes:

HepG2 (Human liver hepatocellular carcinoma) cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 97 kDa

true

Exposure time: 3min

Supplier Data

Western blot - Anti-DMGDH antibody [EPR16908] (AB198292)

5% NFDM/TBST : Blocking and diluting buffer.

All lanes:

Western blot - Anti-DMGDH antibody [EPR16908] (ab198292) at 1/10000 dilution

Lane 1:

Human fetal kidney tissue lysate at 10 µg

Lane 2:

Human fetal liver tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 97 kDa

true

Exposure time: 2s

Carrier free

Anti-DMGDH antibody [EPR16908] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16908

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DMGDH also known as dimethylglycine dehydrogenase is a mitochondrial enzyme with a mass of approximately 103 kDa. This protein is expressed predominantly in liver and kidney tissues. DMGDH plays an important role in the degradation of choline where it catalyzes the oxidative demethylation of dimethylglycine into sarcosine while producing formaldehyde and hydrogen peroxide. This process is an important step in the choline oxidation pathway which helps maintain the methylation balance in the cell.

Biological function summary

DMGDH contributes to the one-carbon metabolism pathway which involves the transfer of one-carbon units essential for various biosynthetic reactions. It does not form part of a larger enzymatic complex but operates as an independent enzyme within the mitochondrial matrix. Its activity is important for methyl cycle function influencing processes like nucleotide synthesis and amino acid metabolism. The enzyme helps connect choline metabolism to broader metabolic networks impacting cellular methyl group availability.

Pathways

DMGDH is integral to the choline metabolism pathway and the one-carbon metabolic pathway. Choline is oxidized to produce substrates for the methionine cycle affecting homocysteine levels and S-adenosylmethionine production. DMGDH's function is closely linked to other enzymes like dimethylglycine N-methyltransferase (DNMT) which also engages in methyl group transfer. Together these enzymes collaborate to maintain cellular methylation and detoxification pathways.

DMGDH dysfunction has been associated with metabolic conditions such as homocystinuria and cardiovascular disease. Impaired DMGDH activity can lead to increased levels of dimethylglycine which in turn may disrupt methylation balance and contribute to metabolic abnormalities. While DMGDH does not directly interact with any known disease-specific proteins its role in maintaining proper methylation highlights its importance in disease pathology. By influencing levels of substrates like homocysteine DMGDH indirectly connects to disorders linked to methylation dysregulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the demethylation of N,N-dimethylglycine to sarcosine. Also has activity with sarcosine in vitro.

See full target information Dimethylglycine dehydrogenase, mitochondrial

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113113 PubMed40792019

2025

NXF1 suppresses progression of endometrial cancer by interacting with the SRSF3 to regulate SP4 splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Meng,Lingjun Li,Xueqin Yang,Hui Tang,Jizhong Wang,Songbai Zhang,Zongbao Xia,Jia Yao,Qi Zhang,Changrong Hu,Chunjie Su,Rui Duan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DMGDH antibody [EPR16908]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMGDH antibody [EPR16908] (AB198292)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMGDH antibody [EPR16908] (AB198292)

Immunoprecipitation - Anti-DMGDH antibody [EPR16908] (AB198292)

Western blot - Anti-DMGDH antibody [EPR16908] (AB198292)

Western blot - Anti-DMGDH antibody [EPR16908] (AB198292)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise