Anti-DMPO Nitrone Adduct antibody

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The DMPO nitrone adduct also known as 55-Dimethyl-1-Pyrroline N-Oxide adduct plays an important role in trapping free radicals during biological reactions. As a spin trap DMPO captures highly reactive radicals like hydroxyl and superoxide radicals converting them to more stable radical adducts that can be detected by Electron Spin Resonance (ESR) spectroscopy. The mass of the DMPO nitrone adduct varies depending on the specific radical bound but the core structure of DMPO nitrone has a molecular weight of 113.15 g/mol. DMPO adducts are expressed in conditioned systems and are especially useful in detecting radical species in cellular environments.

Biological function summary

DMPO nitrone adduct formation is critical for investigating oxidative stress within cells. It does not form part of a complex but acts as an independent trap for free radicals. By stabilizing reactive radicals DMPO adducts help to provide a clearer picture of the radical landscape within biological systems. This insight is essential for understanding the balance between reactive oxygen species production and antioxidant defense mechanisms in various cell types and tissues.

Pathways

DMPO nitrone adduct formation links closely to pathways involving oxidative stress and redox signaling. In these pathways DMPO assists in monitoring dynamic changes and interactions between radicals and antioxidant proteins like superoxide dismutase (SOD) and catalase. These pathways are important for maintaining cellular health and preventing oxidative damage.

Associated diseases and disorders

The use of DMPO nitrone adduct is significant for research on oxidative stress-related conditions. It is connected to diseases like Alzheimer's and cardiovascular disorders where oxidative stress and free radical damage play a well-documented role. The formation of DMPO adducts provides a method for assessing the extent of radical involvement offering insights into disease mechanisms. The interaction between DMPO and proteins such as amyloid-beta in Alzheimer's highlights the importance of free radical monitoring in understanding disease progression.