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AB23702

Anti-DMPO Nitrone Adduct antibody

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(7 Publications)

Rabbit Polyclonal DMPO Nitrone Adduct antibody. Suitable for ELISA, WB and reacts with Chemical samples. Cited in 7 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to DMPO Nitrone Adduct.
1 Images
Western blot - Anti-DMPO Nitrone Adduct antibody (AB23702)
  • WB

Unknown

Western blot - Anti-DMPO Nitrone Adduct antibody (AB23702)

ab23702 was used at a concentration of 1/1000 or 1/5000, as noted at the top of the image.

All lanes:

Western blot - Anti-DMPO Nitrone Adduct antibody (ab23702)

Lane 1:

Metmyoglobin at 3 µL

Lane 2:

Metmyoglobin-DMPO at 3 µL

Lane 3:

Metmyoglobin-DMPO at 5 µL

Lane 4:

Metmyoglobin-DMPO at 10 µL

Observed band size: 17 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Applications

WB, ELISA

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/1000 - 1/5000", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" } } }
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Product details

Measurement of protein radicals is now possible with the development of the "spin trap immunoassay" (please see refs 1,2). Compared to traditional techniques, immunodetection of spin trap products allows a higher level of sensitivity and throughput with greatly reduced sample consumption.
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Constituents: Whole serum
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DMPO nitrone adduct also known as 55-Dimethyl-1-Pyrroline N-Oxide adduct plays an important role in trapping free radicals during biological reactions. As a spin trap DMPO captures highly reactive radicals like hydroxyl and superoxide radicals converting them to more stable radical adducts that can be detected by Electron Spin Resonance (ESR) spectroscopy. The mass of the DMPO nitrone adduct varies depending on the specific radical bound but the core structure of DMPO nitrone has a molecular weight of 113.15 g/mol. DMPO adducts are expressed in conditioned systems and are especially useful in detecting radical species in cellular environments.
Biological function summary

DMPO nitrone adduct formation is critical for investigating oxidative stress within cells. It does not form part of a complex but acts as an independent trap for free radicals. By stabilizing reactive radicals DMPO adducts help to provide a clearer picture of the radical landscape within biological systems. This insight is essential for understanding the balance between reactive oxygen species production and antioxidant defense mechanisms in various cell types and tissues.

Pathways

DMPO nitrone adduct formation links closely to pathways involving oxidative stress and redox signaling. In these pathways DMPO assists in monitoring dynamic changes and interactions between radicals and antioxidant proteins like superoxide dismutase (SOD) and catalase. These pathways are important for maintaining cellular health and preventing oxidative damage.

The use of DMPO nitrone adduct is significant for research on oxidative stress-related conditions. It is connected to diseases like Alzheimer's and cardiovascular disorders where oxidative stress and free radical damage play a well-documented role. The formation of DMPO adducts provides a method for assessing the extent of radical involvement offering insights into disease mechanisms. The interaction between DMPO and proteins such as amyloid-beta in Alzheimer's highlights the importance of free radical monitoring in understanding disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information DMPO Nitrone Adduct
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Current protocols 1:e262 PubMed34570435

2021

Immuno-Spin Trapping Method for the Analysis of S-Nitrosylated Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Esha Sircar,Detcho A Stoyanovsky,Timothy R Billiar,Rajib Sengupta

Frontiers in immunology 9:938 PubMed29867936

2018

Immuno-Spin Trapping-Based Detection of Oxidative Modifications in Cardiomyocytes and Coronary Endothelium in the Progression of Heart Failure in Tgαq*44 Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Bartosz Proniewski,Joanna Czarny,Tamara I Khomich,Kamil Kus,Agnieszka Zakrzewska,Stefan Chlopicki

PloS one 12:e0181732 PubMed28732060

2017

Formation of singlet oxygen by decomposition of protein hydroperoxide in photosystem II.

Applications

WB

Species

Spinacia oleracea

Vinay Pathak,Ankush Prasad,Pavel Pospíšil

Proceedings of the National Academy of Sciences of 114:2988-2993 PubMed28265052

2017

Amino acid oxidation of the D1 and D2 proteins by oxygen radicals during photoinhibition of Photosystem II.

Applications

WB

Species

Unspecified reactive species

Ravindra Kale,Annette E Hebert,Laurie K Frankel,Larry Sallans,Terry M Bricker,Pavel Pospíšil

Free radical biology & medicine 46:853-65 PubMed19159679

2009

Immuno-spin trapping of protein and DNA radicals: "tagging" free radicals to locate and understand the redox process.

Applications

ELISA

Species

Unspecified reactive species

Sandra E Gomez-Mejiba,Zili Zhai,Hammad Akram,Leesa J Deterding,Kenneth Hensley,Nataliya Smith,Rheal A Towner,Kenneth B Tomer,Ronald P Mason,Dario C Ramirez

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 22:215-24 PubMed17724253

2007

Acetaminophen inhibits prostanoid synthesis by scavenging the PGHS-activator peroxynitrite.

Applications

WB

Species

Unspecified reactive species

Stefan Schildknecht,Andreas Daiber,Sandro Ghisla,Richard A Cohen,Markus M Bachschmid

Nature protocols 2:512-22 PubMed17406615

2007

Immuno-spin trapping analyses of DNA radicals.

Applications

Unspecified application

Species

Unspecified reactive species

Dario C Ramirez,Sandra E Gomez-Mejiba,Ronald P Mason
View all publications
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Product promise

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