AB104902

Anti-DMPO Nitrone Adduct antibody [N1664A]

Name: Anti-DMPO Nitrone Adduct antibody [N1664A] (ab104902) | Abcam
Brand: Abcam Limited
SKU: AB104902
Price: 510 USD
Availability: InStock

Be the first to review this product! Submit a review

(4 Publications)

Mouse Monoclonal DMPO Nitrone Adduct antibody. Suitable for WB, IP, ELISA, ICC/IF and reacts with Chemical samples. Cited in 4 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to DMPO Nitrone Adduct.

View Alternative Names

55 dimethyl 1 pyrroline N oxide nitrone adduct

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-DMPO Nitrone Adduct antibody [N1664A] (AB104902)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DMPO Nitrone Adduct antibody [N1664A] (AB104902)

Immuncytochemistry/Immunofluorescence analysis of Mouse macrophage cells labelling DMPO Nitrone Adduct with ab104902 at 10μg/mL. Left - with DMPO, Right - no DMPO. Top to bottom - DMPO, DAPI, overlay.

Unknown

Western blot - Anti-DMPO Nitrone Adduct antibody [N1664A] (AB104902)

All lanes:

Western blot - Anti-DMPO Nitrone Adduct antibody [N1664A] (ab104902) at 10 µg/mL

Lane 1:

10µM Hb

Lane 2:

10µM Hb + 100µM HOCL

Lane 3:

10µM Hb + 500µM HOCL

Lane 4:

10µM Hb + 1000µM HOCL

Lane 5:

10µM Hb with 20mM DMPO

Lane 6:

10µM Hb + 100µM HOCL with 20mM DMPO

Lane 7:

10µM Hb + 500µM HOCL with 20mM DMPO

Lane 8:

10µM Hb + 1000µM HOCL with 20mM DMPO

Lane 9:

10µM Hb with 100mM DMPO

Lane 10:

10µM Hb + 100µM HOCL with 100mM DMPO

Lane 11:

10µM Hb + 500µM HOCL with 100mM DMPO

Lane 12:

10µM Hb + 1000µM HOCL with 100mM DMPO

false

519 FITC

FITC Anti-DMPO Nitrone Adduct antibody [N1664A]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

N1664A

Isotype

IgG1

Carrier free

Applications

ICC/IF, WB, IP, ELISA

applications

Specificity

Detects ~90kDa. Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross-react with non-adducted proteins or DNA.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-10 µg/mL", "WB-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "Follow the method described by Chatterjee, S. et. al. PubMed 19049863.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1-10 µg/mL", "ELISA-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-10 µg/mL", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.05% Sodium azide Constituents: Tris buffered saline, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DMPO nitrone adduct also known as 55-Dimethyl-1-Pyrroline N-Oxide adduct plays an important role in trapping free radicals during biological reactions. As a spin trap DMPO captures highly reactive radicals like hydroxyl and superoxide radicals converting them to more stable radical adducts that can be detected by Electron Spin Resonance (ESR) spectroscopy. The mass of the DMPO nitrone adduct varies depending on the specific radical bound but the core structure of DMPO nitrone has a molecular weight of 113.15 g/mol. DMPO adducts are expressed in conditioned systems and are especially useful in detecting radical species in cellular environments.

Biological function summary

DMPO nitrone adduct formation is critical for investigating oxidative stress within cells. It does not form part of a complex but acts as an independent trap for free radicals. By stabilizing reactive radicals DMPO adducts help to provide a clearer picture of the radical landscape within biological systems. This insight is essential for understanding the balance between reactive oxygen species production and antioxidant defense mechanisms in various cell types and tissues.

Pathways

DMPO nitrone adduct formation links closely to pathways involving oxidative stress and redox signaling. In these pathways DMPO assists in monitoring dynamic changes and interactions between radicals and antioxidant proteins like superoxide dismutase (SOD) and catalase. These pathways are important for maintaining cellular health and preventing oxidative damage.

The use of DMPO nitrone adduct is significant for research on oxidative stress-related conditions. It is connected to diseases like Alzheimer's and cardiovascular disorders where oxidative stress and free radical damage play a well-documented role. The formation of DMPO adducts provides a method for assessing the extent of radical involvement offering insights into disease mechanisms. The interaction between DMPO and proteins such as amyloid-beta in Alzheimer's highlights the importance of free radical monitoring in understanding disease progression.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information DMPO Nitrone Adduct

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 63:102733 PubMed37172395

2023

Temporary alteration of neuronal network communication is a protective response to redox imbalance that requires GPI-anchored prion protein.

Applications

Unspecified application

Species

Unspecified reactive species

Simote T Foliaki,Aleksandar Wood,Katie Williams,Anna Smith,Ryan O Walters,Chase Baune,Bradley R Groveman,Cathryn L Haigh

Cell death discovery 8:95 PubMed35246523

2022

Edonerpic maleate regulates glutamate receptors through CRMP2- and Arc-mediated mechanisms in response to brain trauma.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Chen,Li-Kun Yang,Pu Ai,Jie Zhu,Chun-Hua Hang,Yu-Hai Wang

Cell reports 32:108131 PubMed32937126

2020

Single Nucleotide Resolution Analysis Reveals Pervasive, Long-Lasting DNA Methylation Changes by Developmental Exposure to a Mitochondrial Toxicant.

Applications

Unspecified application

Species

Unspecified reactive species

Oswaldo A Lozoya,Fuhua Xu,Dagoberto Grenet,Tianyuan Wang,Sara A Grimm,Veronica Godfrey,Suramya Waidyanatha,Richard P Woychik,Janine H Santos

Nucleic acids research 43:6334-47 PubMed26082495

2015

Targeted DNA damage at individual telomeres disrupts their integrity and triggers cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Luxi Sun,Rong Tan,Jianquan Xu,Justin LaFace,Ying Gao,Yanchun Xiao,Myriam Attar,Carola Neumann,Guo-Min Li,Bing Su,Yang Liu,Satoshi Nakajima,Arthur S Levine,Li Lan

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DMPO Nitrone Adduct antibody [N1664A]

Immunocytochemistry/ Immunofluorescence - Anti-DMPO Nitrone Adduct antibody [N1664A] (AB104902)

Western blot - Anti-DMPO Nitrone Adduct antibody [N1664A] (AB104902)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise