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AB140977

Anti-DMT1 antibody

4

(3 Reviews)

|

(3 Publications)

Rabbit Polyclonal DMT1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human SLC11A2.

View Alternative Names

DCT1, DMT1, NRAMP2, OK/SW-cl.20, SLC11A2, Natural resistance-associated macrophage protein 2, NRAMP 2, Divalent cation transporter 1, Divalent metal transporter 1, Solute carrier family 11 member 2, DMT-1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMT1 antibody (AB140977)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMT1 antibody (AB140977)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling DMT1 with ab140977 at 20 µg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMT1 antibody (AB140977)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMT1 antibody (AB140977)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling DMT1 with ab140977 at 20 µg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human SLC11A2. The exact immunogen used to generate this antibody is proprietary information.

P49281

Specificity

BLAST analysis of the peptide immunogen showed no homology with other Human proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "15-20 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Common marmoset": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DMT1 protein also known as divalent metal transporter 1 or NRAMP2 functions as an essential transporter for divalent metal ions including iron and manganese. DMT1 has a molecular mass around 65 kDa and is expressed widely in tissues like the intestine liver and brain where it facilitates the uptake of these ions. In the gut it plays a significant role in iron absorption from dietary sources. DMT1 protein contributes to maintaining systemic iron levels important for many physiological processes.
Biological function summary

DMT1 facilitates cellular uptake of iron important for oxygen transport and DNA synthesis. As part of its function DMT1 operates independently not as a component of a larger protein complex enabling it to transport metal ions across cell membranes directly. This process ensures that cells receive necessary ions for their metabolic activities and energy production aiding in cellular homeostasis.

Pathways

DMT1 integrates into important biological systems like the iron metabolism pathway and the manganese transport pathway. It works alongside ferritin a protein responsible for storing iron within cells to regulate iron availability and prevent toxicity. Similarly its role in the manganese transport pathway helps maintain manganese homeostasis which protease enzymes and neurotransmitter synthesis rely on.

DMT1 shows significant relevance to iron overload conditions such as hemochromatosis and neurodegenerative diseases like Parkinson’s disease. Mutations or dysregulation in DMT1 expression can lead to improper iron accumulation contributing to oxidative stress and neuronal damage respectively. Its role in iron uptake and regulation therefore connects it with proteins like hepcidin influential in maintaining iron balance impacting these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Proton-coupled metal ion symporter operating with a proton to metal ion stoichiometry of 1 : 1 (PubMed : 17109629, PubMed : 17293870, PubMed : 22736759, PubMed : 25326704, PubMed : 25491917). Selectively transports various divalent metal cations, in decreasing affinity : Cd(2+) > Fe(2+) > Co(2+), Mn(2+) >> Zn(2+), Ni(2+), VO(2+) (PubMed : 17109629, PubMed : 17293870, PubMed : 22736759, PubMed : 25326704, PubMed : 25491917). Essential for maintenance of iron homeostasis by modulating intestinal absorption of dietary Fe(2+) and TF-associated endosomal Fe(2+) transport in erythroid precursors and other cells (By similarity). Enables Fe(2+) and Mn(2+) ion entry into mitochondria, and is thus expected to promote mitochondrial heme synthesis, iron-sulfur cluster biogenesis and antioxidant defense (By similarity) (PubMed : 24448823). Can mediate uncoupled fluxes of either protons or metal ions.
See full target information SLC11A2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Histology and histopathology 38:1009-1016 PubMed36861878

2023

Treatment of berberine alleviates diabetic nephropathy by reducing iron overload and inhibiting oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Yujing Wang,Shuling Yue,Feng Cai,Wen Zhu,Yuxiang Zhong,Juanjuan Chen,Chunyun Li

Frontiers in endocrinology 10:46 PubMed30800096

2019

Nutritional Intervention for Developmental Brain Damage: Effects of Lactoferrin Supplementation in Hypocaloric Induced Intrauterine Growth Restriction Rat Pups.

Applications

Unspecified application

Species

Unspecified reactive species

Yohan van de Looij,Camille Larpin,Jan-Harry Cabungcal,Eduardo F Sanches,Audrey Toulotte,Kim Q Do,Stéphane V Sizonenko

Journal of clinical biochemistry and nutrition 61:183-188 PubMed29203959

2017

Cancer cell-specific mitochondrial reactive oxygen species promote non-heme iron uptake and enhance the proliferation of gastric epithelial cancer cell.

Applications

Unspecified application

Species

Unspecified reactive species

Hiromu Ito,Hiromi Kurokawa,Aki Hirayama,Hiroko P Indo,Hideyuki J Majima,Hirofumi Matsui
View all publications
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Product promise

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