AB262715

Anti-DMT1 antibody

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(4 Publications)

Rabbit Polyclonal DMT1 antibody. Suitable for WB and reacts with Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SLC11A2.

View Alternative Names

DCT1, DMT1, NRAMP2, OK/SW-cl.20, SLC11A2, Natural resistance-associated macrophage protein 2, NRAMP 2, Divalent cation transporter 1, Divalent metal transporter 1, Solute carrier family 11 member 2, DMT-1

1 Images

Supplier Data

Western blot - Anti-DMT1 antibody (AB262715)

7.5% SDS-PAGE.

All lanes:

Western blot - Anti-DMT1 antibody (ab262715) at 1/1000 dilution

All lanes:

Mouse brain tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 62 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

Recombinant Fragment Protein within Human SLC11A2. The exact immunogen used to generate this antibody is proprietary information.

P49281

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DMT1 protein also known as divalent metal transporter 1 or NRAMP2 functions as an essential transporter for divalent metal ions including iron and manganese. DMT1 has a molecular mass around 65 kDa and is expressed widely in tissues like the intestine liver and brain where it facilitates the uptake of these ions. In the gut it plays a significant role in iron absorption from dietary sources. DMT1 protein contributes to maintaining systemic iron levels important for many physiological processes.

Biological function summary

DMT1 facilitates cellular uptake of iron important for oxygen transport and DNA synthesis. As part of its function DMT1 operates independently not as a component of a larger protein complex enabling it to transport metal ions across cell membranes directly. This process ensures that cells receive necessary ions for their metabolic activities and energy production aiding in cellular homeostasis.

Pathways

DMT1 integrates into important biological systems like the iron metabolism pathway and the manganese transport pathway. It works alongside ferritin a protein responsible for storing iron within cells to regulate iron availability and prevent toxicity. Similarly its role in the manganese transport pathway helps maintain manganese homeostasis which protease enzymes and neurotransmitter synthesis rely on.

DMT1 shows significant relevance to iron overload conditions such as hemochromatosis and neurodegenerative diseases like Parkinson’s disease. Mutations or dysregulation in DMT1 expression can lead to improper iron accumulation contributing to oxidative stress and neuronal damage respectively. Its role in iron uptake and regulation therefore connects it with proteins like hepcidin influential in maintaining iron balance impacting these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Proton-coupled metal ion symporter operating with a proton to metal ion stoichiometry of 1 : 1 (PubMed : 17109629, PubMed : 17293870, PubMed : 22736759, PubMed : 25326704, PubMed : 25491917). Selectively transports various divalent metal cations, in decreasing affinity : Cd(2+) > Fe(2+) > Co(2+), Mn(2+) >> Zn(2+), Ni(2+), VO(2+) (PubMed : 17109629, PubMed : 17293870, PubMed : 22736759, PubMed : 25326704, PubMed : 25491917). Essential for maintenance of iron homeostasis by modulating intestinal absorption of dietary Fe(2+) and TF-associated endosomal Fe(2+) transport in erythroid precursors and other cells (By similarity). Enables Fe(2+) and Mn(2+) ion entry into mitochondria, and is thus expected to promote mitochondrial heme synthesis, iron-sulfur cluster biogenesis and antioxidant defense (By similarity) (PubMed : 24448823). Can mediate uncoupled fluxes of either protons or metal ions.

See full target information SLC11A2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Brain sciences 14: PubMed38790419

2024

Annexin 1 Reduces Dermatitis-Induced Itch and Cholestatic Itch through Inhibiting Neuroinflammation and Iron Overload in the Spinal Dorsal Horn of Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tang Li,Lingyue Hu,Chao Qin,Yuanjie Li,Zhenhua Song,Yang Jiao,Chunyan Wang,Wei Cui,Linlin Zhang

Experimental dermatology 33:e15078 PubMed38610097

2024

Lithium chloride promotes mesenchymal-epithelial transition in murine cutaneous wound healing via inhibiting CXCL9 and IGF2.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Zhang,Xiao Luo,Mianxing Wei,Bingshuai Jing,Jue Wang,Lanling Lin,Bing Shi,Qian Zheng,Chenghao Li

Biomedicines 11: PubMed38001992

2023

High-Altitude Hypoxia Induces Excessive Erythrocytosis in Mice via Upregulation of the Intestinal /Iron-Metabolism Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Sisi Zhou,Jun Yan,Kang Song,Ri-Li Ge

Scientific reports 13:1132 PubMed36670142

2023

SLC11A2: a promising biomarker and therapeutic target in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Liming Tian,Xuemei Li,Huiling Lai,Tingting Sun,Xiaohui Li,Linxiang Wu,Chuling Wu,Shuzhong Yao,Yufeng Ren,Shasha He,Guofen Yang

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-DMT1 antibody

Western blot - Anti-DMT1 antibody (AB262715)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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