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AB262932

Anti-DMT1 antibody

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Rabbit Polyclonal DMT1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SLC11A2 aa 300-400.

View Alternative Names

DCT1, DMT1, NRAMP2, OK/SW-cl.20, SLC11A2, Natural resistance-associated macrophage protein 2, NRAMP 2, Divalent cation transporter 1, Divalent metal transporter 1, Solute carrier family 11 member 2, DMT-1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMT1 antibody (AB262932)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMT1 antibody (AB262932)

Paraffin-embedded human cerebral cortex tissue stained for DMT1 using ab262932 at 1/20 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-DMT1 antibody (AB262932)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DMT1 antibody (AB262932)

PFA-fixed, Triton X-100 permeabilized human U-2OS cells stained for DMT1 (green) using ab262932 at 4 µg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SLC11A2 aa 300-400. The exact immunogen used to generate this antibody is proprietary information.

P49281

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DMT1 protein also known as divalent metal transporter 1 or NRAMP2 functions as an essential transporter for divalent metal ions including iron and manganese. DMT1 has a molecular mass around 65 kDa and is expressed widely in tissues like the intestine liver and brain where it facilitates the uptake of these ions. In the gut it plays a significant role in iron absorption from dietary sources. DMT1 protein contributes to maintaining systemic iron levels important for many physiological processes.
Biological function summary

DMT1 facilitates cellular uptake of iron important for oxygen transport and DNA synthesis. As part of its function DMT1 operates independently not as a component of a larger protein complex enabling it to transport metal ions across cell membranes directly. This process ensures that cells receive necessary ions for their metabolic activities and energy production aiding in cellular homeostasis.

Pathways

DMT1 integrates into important biological systems like the iron metabolism pathway and the manganese transport pathway. It works alongside ferritin a protein responsible for storing iron within cells to regulate iron availability and prevent toxicity. Similarly its role in the manganese transport pathway helps maintain manganese homeostasis which protease enzymes and neurotransmitter synthesis rely on.

DMT1 shows significant relevance to iron overload conditions such as hemochromatosis and neurodegenerative diseases like Parkinson’s disease. Mutations or dysregulation in DMT1 expression can lead to improper iron accumulation contributing to oxidative stress and neuronal damage respectively. Its role in iron uptake and regulation therefore connects it with proteins like hepcidin influential in maintaining iron balance impacting these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Proton-coupled metal ion symporter operating with a proton to metal ion stoichiometry of 1 : 1 (PubMed : 17109629, PubMed : 17293870, PubMed : 22736759, PubMed : 25326704, PubMed : 25491917). Selectively transports various divalent metal cations, in decreasing affinity : Cd(2+) > Fe(2+) > Co(2+), Mn(2+) >> Zn(2+), Ni(2+), VO(2+) (PubMed : 17109629, PubMed : 17293870, PubMed : 22736759, PubMed : 25326704, PubMed : 25491917). Essential for maintenance of iron homeostasis by modulating intestinal absorption of dietary Fe(2+) and TF-associated endosomal Fe(2+) transport in erythroid precursors and other cells (By similarity). Enables Fe(2+) and Mn(2+) ion entry into mitochondria, and is thus expected to promote mitochondrial heme synthesis, iron-sulfur cluster biogenesis and antioxidant defense (By similarity) (PubMed : 24448823). Can mediate uncoupled fluxes of either protons or metal ions.
See full target information SLC11A2
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