Anti-DMT1 antibody [EPR29592-29] - BSA and Azide free

• WB

Lab

Western blot - Anti-DMT1 antibody [EPR29592-29] - BSA and Azide free (AB324359)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Performed under reducing conditions.

In Western blot, ab324349 was shown to bind specifically to DMT1/SLC11A2 . Target of interest was observed at 75-100 kDa in wild-type HeLa cell lysates (lane 1) with no signal observed at this size in DMT1/SLC11A2 knockout cell line (lane 2).

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-DMT1 antibody [EPR29592-29] (<a href='/en-us/products/primary-antibodies/dmt1-antibody-epr29592-29-ab324349'>ab324349</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

DMT1/SLC11A2 knockout HeLa whole cell lysate at 20 µg

Lane 3:

SK-MEL-2 (human skin malignant melanoma cell) whole cell lysate at 20 µg

Lane 4:

SK-MEL-28 (human malignant melanoma cell) whole cell lysate at 20 µg

Lane 5:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 75-100 kDa,36 kDa

false

Exposure time: 92s

• WB

Lab

Western blot - Anti-DMT1 antibody [EPR29592-29] - BSA and Azide free (AB324359)

This data was developed using ab324349, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Samples are non-boiled as boiling may cause protein aggregation.

Lane 2 of this blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-DMT1 antibody [EPR29592-29] (<a href='/en-us/products/primary-antibodies/dmt1-antibody-epr29592-29-ab324349'>ab324349</a>) at 1/1000 dilution

Lane 1:

Human pancreas cancer tissue lysate at 20 µg

Lane 2:

Human small intestine tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 75-100 kDa,36 kDa

true

Exposure time: 180s