Goat Polyclonal DMTF1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Synthetic Peptide within Human DMTF1 aa 600-650.
Images
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human DMTF1 aa 600-650. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y222
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
| Cow | Predicted |
| Dog | Predicted |
| Pig | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1.00000-3.00000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
Species Rat | Dilution info 1.00000-3.00000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
Species Human | Dilution info 1.00000-3.00000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, Pig, Cow | Dilution info - | Notes - |
Target data
Function
Transcriptional activator which activates the CDKN2A/ARF locus in response to Ras-Raf signaling, thereby promoting p53/TP53-dependent growth arrest (By similarity). Binds to the consensus sequence 5'-CCCG[GT]ATGT-3' (By similarity). Isoform 1 may cooperate with MYB to activate transcription of the ANPEP gene. Isoform 2 may antagonize transcriptional activation by isoform 1.
Alternative names
DMP1, DMTF1, Cyclin-D-binding Myb-like transcription factor 1, hDMTF1, Cyclin-D-interacting Myb-like protein 1, hDMP1
Recommended products
Goat Polyclonal DMTF1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Synthetic Peptide within Human DMTF1 aa 600-650.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human DMTF1 aa 600-650. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y222
- Purification technique
- Affinity purification Immunogen
- Specificity
ab177246 is expected to recognize both reported isoforms (NP_066968.3; NP_001135798.1). Reported variants represent identical protein: NP_066968.3, NP_001135799.1.
- Concentration
- Purification notes
ab177246 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DMTF1 also known as Cyclin D-binding Myb-like transcription factor 1 is a transcription factor with a mass of approximately 120 kDa. It plays an important role in cell cycle regulation by interacting with cyclin D. DMTF1 expression is found in multiple tissues with higher levels notable in the prostate and colon. It exerts its mechanical function mainly in the nucleus where it binds to DNA sequences to modulate the transcription of its target genes.
- Biological function summary
DMTF1 influences the cell's response to stress and arrest signals affecting cell proliferation and apoptosis. It acts as part of a transcriptional regulatory complex that involves other proteins such as p14ARF. This association allows DMTF1 to modulate tumor suppressor pathways. Such activity is important for maintaining cellular integrity and preventing uncontrolled cell growth that characterizes cancer.
- Pathways
DMTF1 integrates into key regulatory networks such as the p53 and E2F pathways. In the p53 pathway DMTF1 interacts with other proteins such as MDM2 reinforcing the cell's defense mechanisms against DNA damage. Through the E2F pathway DMTF1 affects the expression of genes controlling cell cycle progression. These pathways link DMTF1 closely to the mechanisms that determine cell fate decisions in response to environmental cues.
- Associated diseases and disorders
DMTF1 shows a significant connection to cancers including lung and prostate cancer. Abnormal activity of DMTF1 can disrupt normal cell cycle control leading to tumorigenesis. Its interaction with proteins like p14ARF and MDM2 further exemplifies its involvement in cancer development. Understanding these interactions allows for deeper insights into potential therapeutic targets for these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Western blot - Anti-DMTF1 antibody (ab177246)
Primary incubation was 1 hour.
All lanes: Western blot - Anti-DMTF1 antibody (ab177246) at 1 µg/mL
Lane 1: Human testis lysate (in RIPA buffer) at 35 µg
Lane 2: Mouse testis lysate (in RIPA buffer) at 35 µg
Lane 3: Rat testis lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 84 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com