JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB177246

Anti-DMTF1 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Goat Polyclonal DMTF1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DMTF1 aa 600-650.

View Alternative Names

DMP1, DMTF1, Cyclin-D-binding Myb-like transcription factor 1, hDMTF1, Cyclin-D-interacting Myb-like protein 1, hDMP1

1 Images
Western blot - Anti-DMTF1 antibody (AB177246)
  • WB

Supplier Data

Western blot - Anti-DMTF1 antibody (AB177246)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-DMTF1 antibody (ab177246) at 1 µg/mL

Lane 1:

Human testis lysate (in RIPA buffer) at 35 µg

Lane 2:

Mouse testis lysate (in RIPA buffer) at 35 µg

Lane 3:

Rat testis lysate (in RIPA buffer) at 35 µg

Predicted band size: 84 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB

applications

Immunogen

Synthetic Peptide within Human DMTF1 aa 600-650. The exact immunogen used to generate this antibody is proprietary information.

Q9Y222

Specificity

ab177246 is expected to recognize both reported isoforms (NP_066968.3; NP_001135798.1). Reported variants represent identical protein: NP_066968.3, NP_001135799.1.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab177246 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DMTF1 also known as Cyclin D-binding Myb-like transcription factor 1 is a transcription factor with a mass of approximately 120 kDa. It plays an important role in cell cycle regulation by interacting with cyclin D. DMTF1 expression is found in multiple tissues with higher levels notable in the prostate and colon. It exerts its mechanical function mainly in the nucleus where it binds to DNA sequences to modulate the transcription of its target genes.
Biological function summary

DMTF1 influences the cell's response to stress and arrest signals affecting cell proliferation and apoptosis. It acts as part of a transcriptional regulatory complex that involves other proteins such as p14ARF. This association allows DMTF1 to modulate tumor suppressor pathways. Such activity is important for maintaining cellular integrity and preventing uncontrolled cell growth that characterizes cancer.

Pathways

DMTF1 integrates into key regulatory networks such as the p53 and E2F pathways. In the p53 pathway DMTF1 interacts with other proteins such as MDM2 reinforcing the cell's defense mechanisms against DNA damage. Through the E2F pathway DMTF1 affects the expression of genes controlling cell cycle progression. These pathways link DMTF1 closely to the mechanisms that determine cell fate decisions in response to environmental cues.

DMTF1 shows a significant connection to cancers including lung and prostate cancer. Abnormal activity of DMTF1 can disrupt normal cell cycle control leading to tumorigenesis. Its interaction with proteins like p14ARF and MDM2 further exemplifies its involvement in cancer development. Understanding these interactions allows for deeper insights into potential therapeutic targets for these diseases.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Transcriptional activator which activates the CDKN2A/ARF locus in response to Ras-Raf signaling, thereby promoting p53/TP53-dependent growth arrest (By similarity). Binds to the consensus sequence 5'-CCCG[GT]ATGT-3' (By similarity). Isoform 1 may cooperate with MYB to activate transcription of the ANPEP gene. Isoform 2 may antagonize transcriptional activation by isoform 1.
See full target information DMTF1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of the American Society of Nephrology : JASN 34:88-109 PubMed36167728

2022

Kidney Organoids Generated Using an Allelic Series of NPHS2 Point Variants Reveal Distinct Intracellular Podocin Mistrafficking.

Applications

Unspecified application

Species

Unspecified reactive species

Aude Dorison,Irene Ghobrial,Alison Graham,Thanushi Peiris,Thomas A Forbes,Michael See,Mithun Das,Moin A Saleem,Catherine Quinlan,Kynan T Lawlor,Mirana Ramialison,Sara E Howden,Melissa H Little
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB259598

Anti-DMP1 antibody [EPR22205-14] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-DMP1 antibody [EPR22205-14] - BSA and Azide free (Detector) (AB259598)

  • 0
  • 0
Primary Antibodies

AB272929

Anti-DSPP antibody

Western blot - Anti-DSPP antibody (AB272929)

  • 0
  • 0
Primary Antibodies

AB34712

Anti-Collagen II antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen II antibody (AB34712)

  • 4
  • 16
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com