Rabbit Polyclonal DMXL2 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human DMXL2 aa 1450-1500.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human DMXL2 aa 1450-1500. The exact immunogen used to generate this antibody is proprietary information. Database link Q8TDJ6
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Use 20 μl/mg lysate. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
May serve as a scaffold protein for MADD and RAB3GA on synaptic vesicles (PubMed:11809763). Plays a role in the brain as a key controller of neuronal and endocrine homeostatic processes (By similarity).
Alternative names
KIAA0856, DMXL2, DmX-like protein 2, Rabconnectin-3
Recommended products
Rabbit Polyclonal DMXL2 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human DMXL2 aa 1450-1500.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human DMXL2 aa 1450-1500. The exact immunogen used to generate this antibody is proprietary information. Database link Q8TDJ6
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab240690 was affinity purified using an epitope specific to DMXL2 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DMXL2 also known as rabconnectin-3α is a large protein with a mass of approximately 350 kDa. It contains multiple WD40 repeats which suggests a role in protein-protein interactions. DMXL2 is expressed in various tissues with higher concentrations in the brain pituitary gland and reproductive organs. It functions mechanically in intracellular transport and signal transduction influencing neurotransmitter release and hormone secretion.
- Biological function summary
DMXL2 plays a role in cellular homeostasis and communication. It is part of a protein complex associating with rab3 GTPase-activating proteins to regulate synaptic vesicle exocytosis. This protein contributes to the balance of Ca2+ dynamics in neuronal cells therefore influencing neurotransmitter release processes. Because it interacts directly with components involved in signaling DMXL2 impacts cellular responses to external stimuli.
- Pathways
DMXL2 participates in the Notch signaling pathway and mTOR pathway. This protein interacts with key molecules such as rab3 a small GTPase essential for vesicle trafficking. The contribution of DMXL2 to these pathways enables it to orchestrate critical steps in cell growth proliferation and survival. By engaging in these pathways DMXL2 influences cellular metabolism and synaptic plasticity.
- Associated diseases and disorders
DMXL2 shows a connection to polycystic ovary syndrome (PCOS) and neurodegenerative disorders like Alzheimer's disease. Altered DMXL2 expression or function impacts reproductive endocrinology pathways related to PCOS where it can interact with proteins like FSH receptor. In Alzheimer's disease DMXL2's role in synaptic function ties it to amyloid-beta peptide processes an important aspect of the disease's progression. These connections highlight DMXL2 as a potential target for therapeutic intervention in these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-DMXL2 antibody (ab240690)
Lysates prepared using NETN lysis buffer.
All lanes: Western blot - Anti-DMXL2 antibody (ab240690) at 1/1000 dilution
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 3: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 340 kDa
Exposure time: 3min
Immunoprecipitation - Anti-DMXL2 antibody (ab240690)
DMXL2 immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer. Western blot was performed from the immunoprecipitate using ab240690 at 1/100 dilution.
Lane 1: ab240690 used for IP at 20 μl per reaction in Jurkat whole cell lysate.
Lane 2: Control IgG iP in Jurkat whole cell lysate.Detection: Chemiluminescence with an exposure time of 30 seconds.
All lanes: Immunoprecipitation - Anti-DMXL2 antibody (ab240690)
Predicted band size: 340 kDa
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