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AB54399

Anti-DNA helicase HEL308 antibody [2406C1a]

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(1 Publication)

Mouse Monoclonal DNA helicase HEL308 antibody. Suitable for WB and reacts with Recombinant fragment samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HELQ.

View Alternative Names

HEL308, HELQ, Helicase POLQ-like, Mus308-like helicase, POLQ-like helicase

1 Images
Western blot - Anti-DNA helicase HEL308 antibody [2406C1a] (AB54399)
  • WB

Unknown

Western blot - Anti-DNA helicase HEL308 antibody [2406C1a] (AB54399)

Western blot analysis of immunised recombinant protein fragment, using ab54399

All lanes:

Western blot - Anti-DNA helicase HEL308 antibody [2406C1a] (ab54399)

Predicted band size: 124 kDa

Observed band size: 40 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2406C1a

Isotype

IgG1

Carrier free

No

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human HELQ. The exact immunogen used to generate this antibody is proprietary information.

Q8TDG4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab54399 was filtered through a 0.22 µm membrane.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Single-stranded 3'-5' DNA helicase that plays a key role in homology-driven double-strand break (DSB) repair (PubMed : 11751861, PubMed : 19995904, PubMed : 21398521, PubMed : 24005041, PubMed : 24005565, PubMed : 34316696, PubMed : 34937945). Involved in different DSB repair mechanisms that are guided by annealing of extensive stretches of complementary bases at break ends, such as microhomology-mediated end-joining (MMEJ), single-strand annealing (SSA) or synthesis-dependent strand annealing (SDSA) (PubMed : 34937945). Possesses both DNA unwinding and annealing activities (PubMed : 34937945). Forms a complex with RAD51, stimulating HELQ DNA helicase activity and ability to unwing DNA (PubMed : 34937945). Efficiently unwinds substrates containing 3' overhangs or a D-loop (PubMed : 21398521, PubMed : 34937945). In contrast, interaction with the replication protein A (RPA/RP-A) complex inhibits DNA unwinding by HELQ but strongly stimulates DNA strand annealing (PubMed : 34937945). Triggers displacement of RPA from single-stranded DNA to facilitate annealing of complementary sequences (PubMed : 34316696, PubMed : 34937945).
See full target information HELQ
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 16:29923-35 PubMed26694360

2015

DNA Double Strand Break Response and Limited Repair Capacity in Mouse Elongated Spermatids.

Applications

Unspecified application

Species

Unspecified reactive species

Emad A Ahmed,Harry Scherthan,Dirk G de Rooij
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Product promise

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