AB96576

Anti-DNA Ligase III/LIG3 antibody

Name: Anti-DNA Ligase III/LIG3 antibody (ab96576) | Abcam
Brand: Abcam Limited
SKU: AB96576
Rating: 5 (1 reviews)

(1 Review)

(2 Publications)

Rabbit Polyclonal DNA Ligase III/LIG3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LIG3 aa 750-1000.

View Alternative Names

DNA ligase 3, DNA ligase III, Polydeoxyribonucleotide synthase [ATP] 3, LIG3

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Ligase III/LIG3 antibody (AB96576)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Ligase III/LIG3 antibody (AB96576)

Immunohistochemical analysis of Ligase III in paraffin embedded H441 Xenograft, using ab96576 at a 1/100 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-DNA Ligase III/LIG3 antibody (AB96576)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DNA Ligase III/LIG3 antibody (AB96576)

Immunofluorescence analysis of Ligase III in paraformaldehyde fixed A431, using ab96576 at a 1/200 dilution.
Lower image (2) : merged with DNA probe.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DNA Ligase III/LIG3 antibody (AB96576)

Immunofluorescence analysis of 4% paraformaldehyde fixed at RT for 15 min HeLa cells labelling DNA ligase III protein at nucleus using ab96576 at a 1/200 dilution (Green). Phalloidin, a cytoskeleton marker stained by anti-phalloidin antibody at a 1/200 dilution (Red). Scale bar= 10 µm.

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Western blot - Anti-DNA Ligase III/LIG3 antibody (AB96576)

7.5% SDS PAGE

All lanes:

Western blot - Anti-DNA Ligase III/LIG3 antibody (ab96576) at 1/1000 dilution

All lanes:

MOLT4 whole cell lysate at 30 µg

Predicted band size: 113 kDa

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Supplier Data

Western blot - Anti-DNA Ligase III/LIG3 antibody (AB96576)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-DNA Ligase III/LIG3 antibody (ab96576) at 1/500 dilution

Lane 1:

HepG2 Whole cell extract at 30 µg

Lane 2:

HepG2 nuclear extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 113 kDa

false

Supplier Data

Western blot - Anti-DNA Ligase III/LIG3 antibody (AB96576)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-DNA Ligase III/LIG3 antibody (ab96576) at 1/500 dilution

Lane 1:

Non-transfected (-) 293T whole cell extracts at 30 µg

Lane 2:

Transfected (+) 293T whole cell extracts at 30 µg

Predicted band size: 113 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human LIG3 aa 750-1000. The exact immunogen used to generate this antibody is proprietary information.

P49916

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Antigen retrieval is not essential but may optimise staining.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DNA Ligase III also known as LIG3 or Lig 3 is an enzyme that plays a central role in DNA repair by catalyzing the joining of DNA strands. Mechanically it facilitates the ligation step in the DNA repair process sealing nicks in the DNA backbone. This ligase has a sizing mass of approximately 103 kDa. It is expressed widely in human cells with a high concentration in tissues with active DNA repair needs such as testes and ovaries. Ligase acts in various subcellular locations including the nucleus and mitochondria with distinct isoforms functioning in these compartments.

Biological function summary

DNA Ligase III is significant in maintaining genomic stability. It often operates as part of multi-protein complexes interacting especially with XRCC1 during base excision repair (BER). It ensures the continuity of DNA strands after damage-induced breaks are processed. LIG3 also has a pivotal function in the mitochondria where it is involved in repair and replication of mitochondrial DNA therefore preserving mitochondrial genome integrity.

Pathways

DNA Ligase III is an integral part of the DNA repair pathways particularly the base excision repair (BER) and single-strand break repair (SSBR) pathways. In the BER pathway LIG3 collaborates closely with other proteins like XRCC1 to mend damaged DNA by filling in missing nucleotides and sealing nicks. It also plays a role in non-homologous end joining (NHEJ) though this is more dominantly accomplished by DNA Ligase IV. These pathways are important for correcting oxidative damage and abasic sites that can disrupt the DNA structure.

DNA Ligase III has associations with certain syndromes and cancers. Mutations or malfunctions in this enzyme can lead to ataxia-telangiectasia-like disorder owing to impaired DNA repair processes. Additionally LIG3 expression is often evaluated in the context of cancerous cells where aberrant repair processes could promote tumor progression. A connection with XRCC1 another key player in the repair machinery is critical as XRCC1 mutations may magnify the impact of LIG3 deficiencies contributing to disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Isoform 3 functions as a heterodimer with DNA-repair protein XRCC1 in the nucleus and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Isoform 1 is targeted to mitochondria, where it functions as a DNA ligase in mitochondrial base-excision DNA repair (PubMed : 10207110, PubMed : 24674627).

See full target information LIG3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 25:103537 PubMed34977502

2022

DOCK8-expressing T follicular helper cells newly generated beyond self-organized criticality cause systemic lupus erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

Shunichi Shiozawa,Ken Tsumiyama,Yumi Miyazaki,Kenichi Uto,Keiichi Sakurai,Toshie Nakashima,Hiroko Matsuyama,Ai Doi,Miho Tarui,Manabu Izumikawa,Mai Kimura,Yuko Fujita,Chisako Satonaka,Takahiko Horiuchi,Tsukasa Matsubara,Motohiro Oribe,Takashi Yamane,Hidetoshi Kagawa,Quan-Zhen Li,Keiko Mizuno,Yohei Mukai,Kazuhiro Murakami,Takuji Enya,Shota Tsukimoto,Yoshiyuki Hakata,Masaaki Miyazawa,Kazuko Shiozawa

Nature communications 11:5775 PubMed33188175

2020

CHD7 and 53BP1 regulate distinct pathways for the re-ligation of DNA double-strand breaks.

Applications

Unspecified application

Species

Unspecified reactive species

Magdalena B Rother,Stefania Pellegrino,Rebecca Smith,Marco Gatti,Cornelia Meisenberg,Wouter W Wiegant,Martijn S Luijsterburg,Ralph Imhof,Jessica A Downs,Alfred C O Vertegaal,Sébastien Huet,Matthias Altmeyer,Haico van Attikum

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DNA Ligase III/LIG3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Ligase III/LIG3 antibody (AB96576)

Immunocytochemistry/ Immunofluorescence - Anti-DNA Ligase III/LIG3 antibody (AB96576)

Immunocytochemistry/ Immunofluorescence - Anti-DNA Ligase III/LIG3 antibody (AB96576)

Western blot - Anti-DNA Ligase III/LIG3 antibody (AB96576)

Western blot - Anti-DNA Ligase III/LIG3 antibody (AB96576)

Western blot - Anti-DNA Ligase III/LIG3 antibody (AB96576)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise