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AB97404

Anti-DNA Ligase III/LIG3 antibody

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(1 Review)

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(1 Publication)

Rabbit Polyclonal DNA Ligase III/LIG3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human LIG3 aa 100-450.

View Alternative Names

DNA ligase 3, DNA ligase III, Polydeoxyribonucleotide synthase [ATP] 3, LIG3

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Ligase III/LIG3 antibody (AB97404)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Ligase III/LIG3 antibody (AB97404)

ab97404, at 1/100 dilution, staining DNA Ligase III/LIG3 in paraffin-embedded A549 xenograft by Immunohistochemistry.

Western blot - Anti-DNA Ligase III/LIG3 antibody (AB97404)
  • WB

Supplier Data

Western blot - Anti-DNA Ligase III/LIG3 antibody (AB97404)

Samples were separated by 5% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program

All lanes:

Western blot - Anti-DNA Ligase III/LIG3 antibody (ab97404) at 1/1000 dilution

Lane 1:

293T Whole cell extract at 30 µg

Lane 2:

A431 Whole cell extract at 30 µg

Lane 3:

HeLa Whole cell extract at 30 µg

Lane 4:

HepG2 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 113 kDa

false

Western blot - Anti-DNA Ligase III/LIG3 antibody (AB97404)
  • WB

Supplier Data

Western blot - Anti-DNA Ligase III/LIG3 antibody (AB97404)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-DNA Ligase III/LIG3 antibody (ab97404) at 1/500 dilution

Lane 1:

Non-transfected (-) 293T whole cell extracts at 30 µg

Lane 2:

Transfected (+) 293T whole cell extracts at 30 µg

Predicted band size: 113 kDa

false

Western blot - Anti-DNA Ligase III/LIG3 antibody (AB97404)
  • WB

Supplier Data

Western blot - Anti-DNA Ligase III/LIG3 antibody (AB97404)

Samples were separated by 7.5% SDS-PAGE.
Observed DNA ligase III protein has multiple bands at 92 kDa, and 110 kDa, possibly due to alternative splicing. PMID : 9001252.

All lanes:

Western blot - Anti-DNA Ligase III/LIG3 antibody (ab97404) at 1/1000 dilution

Lane 1:

293T Whole cell extract at 30 µg

Lane 2:

A431 Whole cell extract at 30 µg

Lane 3:

HeLa Whole cell extract at 30 µg

Lane 4:

HepG2 Whole cell extract at 30 µg

Predicted band size: 113 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human LIG3 aa 100-450. The exact immunogen used to generate this antibody is proprietary information.

P49916

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DNA Ligase III also known as LIG3 or Lig 3 is an enzyme that plays a central role in DNA repair by catalyzing the joining of DNA strands. Mechanically it facilitates the ligation step in the DNA repair process sealing nicks in the DNA backbone. This ligase has a sizing mass of approximately 103 kDa. It is expressed widely in human cells with a high concentration in tissues with active DNA repair needs such as testes and ovaries. Ligase acts in various subcellular locations including the nucleus and mitochondria with distinct isoforms functioning in these compartments.
Biological function summary

DNA Ligase III is significant in maintaining genomic stability. It often operates as part of multi-protein complexes interacting especially with XRCC1 during base excision repair (BER). It ensures the continuity of DNA strands after damage-induced breaks are processed. LIG3 also has a pivotal function in the mitochondria where it is involved in repair and replication of mitochondrial DNA therefore preserving mitochondrial genome integrity.

Pathways

DNA Ligase III is an integral part of the DNA repair pathways particularly the base excision repair (BER) and single-strand break repair (SSBR) pathways. In the BER pathway LIG3 collaborates closely with other proteins like XRCC1 to mend damaged DNA by filling in missing nucleotides and sealing nicks. It also plays a role in non-homologous end joining (NHEJ) though this is more dominantly accomplished by DNA Ligase IV. These pathways are important for correcting oxidative damage and abasic sites that can disrupt the DNA structure.

DNA Ligase III has associations with certain syndromes and cancers. Mutations or malfunctions in this enzyme can lead to ataxia-telangiectasia-like disorder owing to impaired DNA repair processes. Additionally LIG3 expression is often evaluated in the context of cancerous cells where aberrant repair processes could promote tumor progression. A connection with XRCC1 another key player in the repair machinery is critical as XRCC1 mutations may magnify the impact of LIG3 deficiencies contributing to disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Isoform 3 functions as a heterodimer with DNA-repair protein XRCC1 in the nucleus and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Isoform 1 is targeted to mitochondria, where it functions as a DNA ligase in mitochondrial base-excision DNA repair (PubMed : 10207110, PubMed : 24674627).
See full target information LIG3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of leukocyte biology 82:551-8 PubMed17576823

2007

Intracellular pools of FcalphaR (CD89) in human neutrophils are localized in tertiary granules and secretory vesicles, and two FcalphaR isoforms are found in tertiary granules.

Applications

Unspecified application

Species

Unspecified reactive species

Na Yin,Min Peng,Yukun Xing,Wei Zhang
View all publications
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Product promise

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